Studies on bactericidal efficacy of pumpkin (Cucurbita moschata Duchesne) peel

Objective: To explore the in vitro antibacterial potential of the peel of Cucurbita moschata Duchesne (tropical pumpkin) (C. moschata) against human pathogenic bacteria. Methods: In the present study, dichloromethane (DCM), methanol (MEOH) and aqueous (AQ) extracts of C. moschata peel were examine...

Full description

Bibliographic Details
Main Author:	Ahmed, Qamar Uddin
Format:	Article
Language:	English
Published:	Coastal Life Medicine Media co., Limited 2014
Subjects:	RC86 First aid in illness and injury
Online Access:	http://irep.iium.edu.my/36382/ http://irep.iium.edu.my/36382/ http://irep.iium.edu.my/36382/ http://irep.iium.edu.my/36382/1/Studies_on_bactericidal_efficacy_of_pumpkin_%28Cucurbita_moschata_Feb_2014.pdf

id	iium-36382
recordtype	eprints
spelling	iium-363822018-06-20T05:17:50Z http://irep.iium.edu.my/36382/ Studies on bactericidal efficacy of pumpkin (Cucurbita moschata Duchesne) peel Ahmed, Qamar Uddin RC86 First aid in illness and injury Objective: To explore the in vitro antibacterial potential of the peel of Cucurbita moschata Duchesne (tropical pumpkin) (C. moschata) against human pathogenic bacteria. Methods: In the present study, dichloromethane (DCM), methanol (MEOH) and aqueous (AQ) extracts of C. moschata peel were examined for in vitro antibacterial potency against eight bacterial strains i.e. Bacillus cereus, Burkholderia cepacia, Escherichia coli, Enterococcus faecalis, Staphyloccocus aureus, Pseudomonas aerugenosa, Vibrio alginolyticus, Vibrio parahaemolyticus using Kirby-Bauer disk diffusion susceptibility and broth micro-dilution methods. Results: DCM extract of pumpkin peel exhibited the maximum zone of inhibition against S. aureus (21 mm) whereas aqueous extract of pumpkin peel revealed the least zone of inhibition against E. coli (8 mm). Methanol extract gave maximum zone of inhibition against P. aerugenosa (19 mm). Broth micro-dilution method showed minimum inhibitory concentration (MIC) for the DCM extract against B. cepacia at 6.25 mg/mL. The minimum bactericidal concentrations (MBC) were also determined to know the nature of all extracts. DCM and MEOH extracts exhibited bactericidal nature to all bacterial strains except for the V. alginolyticus. The MBC values exhibited bactericidal nature ranged from 3.12 mg/mL to 100.00 mg/mL. The screening of antimicrobial properties of different extracts of C. moschata peel revealed that the DCM extract possesses good antimicrobial efficacy compared to MEOH and AQ extracts. Conclusions: Peel of C. moschata possesses antibacterial compounds and could be potential source for a new class of antibiotics. Coastal Life Medicine Media co., Limited 2014-02 Article PeerReviewed application/pdf en http://irep.iium.edu.my/36382/1/Studies_on_bactericidal_efficacy_of_pumpkin_%28Cucurbita_moschata_Feb_2014.pdf Ahmed, Qamar Uddin (2014) Studies on bactericidal efficacy of pumpkin (Cucurbita moschata Duchesne) peel. Journal of Coastal Life Medicine, 2 (2). pp. 146-153. ISSN 2309-6152 (O), 2309-5288 (P) http://www.jclmm.com/qk/20142/12.pdf DOI: 10.12980/JCLM.2.201414J7
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RC86 First aid in illness and injury
spellingShingle	RC86 First aid in illness and injury Ahmed, Qamar Uddin Studies on bactericidal efficacy of pumpkin (Cucurbita moschata Duchesne) peel
description	Objective: To explore the in vitro antibacterial potential of the peel of Cucurbita moschata Duchesne (tropical pumpkin) (C. moschata) against human pathogenic bacteria. Methods: In the present study, dichloromethane (DCM), methanol (MEOH) and aqueous (AQ) extracts of C. moschata peel were examined for in vitro antibacterial potency against eight bacterial strains i.e. Bacillus cereus, Burkholderia cepacia, Escherichia coli, Enterococcus faecalis, Staphyloccocus aureus, Pseudomonas aerugenosa, Vibrio alginolyticus, Vibrio parahaemolyticus using Kirby-Bauer disk diffusion susceptibility and broth micro-dilution methods. Results: DCM extract of pumpkin peel exhibited the maximum zone of inhibition against S. aureus (21 mm) whereas aqueous extract of pumpkin peel revealed the least zone of inhibition against E. coli (8 mm). Methanol extract gave maximum zone of inhibition against P. aerugenosa (19 mm). Broth micro-dilution method showed minimum inhibitory concentration (MIC) for the DCM extract against B. cepacia at 6.25 mg/mL. The minimum bactericidal concentrations (MBC) were also determined to know the nature of all extracts. DCM and MEOH extracts exhibited bactericidal nature to all bacterial strains except for the V. alginolyticus. The MBC values exhibited bactericidal nature ranged from 3.12 mg/mL to 100.00 mg/mL. The screening of antimicrobial properties of different extracts of C. moschata peel revealed that the DCM extract possesses good antimicrobial efficacy compared to MEOH and AQ extracts. Conclusions: Peel of C. moschata possesses antibacterial compounds and could be potential source for a new class of antibiotics.
format	Article
author	Ahmed, Qamar Uddin
author_facet	Ahmed, Qamar Uddin
author_sort	Ahmed, Qamar Uddin
title	Studies on bactericidal efficacy of pumpkin (Cucurbita moschata Duchesne) peel
title_short	Studies on bactericidal efficacy of pumpkin (Cucurbita moschata Duchesne) peel
title_full	Studies on bactericidal efficacy of pumpkin (Cucurbita moschata Duchesne) peel
title_fullStr	Studies on bactericidal efficacy of pumpkin (Cucurbita moschata Duchesne) peel
title_full_unstemmed	Studies on bactericidal efficacy of pumpkin (Cucurbita moschata Duchesne) peel
title_sort	studies on bactericidal efficacy of pumpkin (cucurbita moschata duchesne) peel
publisher	Coastal Life Medicine Media co., Limited
publishDate	2014
url	http://irep.iium.edu.my/36382/ http://irep.iium.edu.my/36382/ http://irep.iium.edu.my/36382/ http://irep.iium.edu.my/36382/1/Studies_on_bactericidal_efficacy_of_pumpkin_%28Cucurbita_moschata_Feb_2014.pdf
first_indexed	2023-09-18T20:52:07Z
last_indexed	2023-09-18T20:52:07Z
_version_	1777410063799943168

Studies on bactericidal efficacy of pumpkin (Cucurbita moschata Duchesne) peel

Similar Items