Comparing BRIN-BD11 culture producing insulin using different type of microcarriers
This research was conducted to examine the growth profile, growth kinetics, and insulinsecretory responsiveness of BRIN-BD11 cells grown in optimized medium on different types of microcarriers (MCs). Comparisons were made on modified polystyrene (Hillex� II) and crosslinked polystyrene Plastic...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2010
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/3639/ http://irep.iium.edu.my/3639/ http://irep.iium.edu.my/3639/ http://irep.iium.edu.my/3639/1/paper_at_cytotechnolopgy_%28dec_2010%29.pdf |
| Summary: | This research was conducted to examine
the growth profile, growth kinetics, and insulinsecretory
responsiveness of BRIN-BD11 cells grown
in optimized medium on different types of microcarriers
(MCs). Comparisons were made on modified
polystyrene (Hillex� II) and crosslinked polystyrene
Plastic Plus (PP) from Solohill Engineering. The cell
line producing insulin was cultured in a 25 cm2 T-flask
as control while MCs based culture was implemented
in a stirred tank bioreactor with 1 L working volume.
For each culture type, the viable cell number, glucose,
lactate, glutamate, and insulin concentrations were
measured and compared. Maximum viable cell number
was obtained at 1.47 9 105 cell/mL for PP
microcarrier (PPMCs) culture, 1.35 9 105 cell/mL
Hillex� II (HIIMCs) culture and 0.95 9 105 cell/mL
for T-flask culture, respectively. The highest insulin
concentration has been produced in PPMCs culture
(5.31 mg/L) compared toHIIMCs culture (2.01 mg/L)
and T-flask culture (1.99 mg/L). Therefore overall
observation suggested that PPMCs was likely preferred
to be used for BRIN-BD11 cell culture as
compared with Hillex� II MCs. |
|---|