Optimization of newcastle disease virus production in t-flask

In this work, the propagation of Asplin F strain of Newcastle disease virus (NDV) in DF-1 cells was investigated. Experiments were carried out in T-flask to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on viru...

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Main Authors:	Arifin, Mohd Azmir, Salim, Siti Hajar, Mel, Maizirwan, Abdul Karim, Mohamed Ismail, Syed Hassan, Sharifah
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	QR355 Virology
Online Access:	http://irep.iium.edu.my/3643/ http://irep.iium.edu.my/3643/ http://irep.iium.edu.my/3643/1/Paper_NDV_Azmir.pdf

id	iium-3643
recordtype	eprints
spelling	iium-36432012-01-19T00:25:56Z http://irep.iium.edu.my/3643/ Optimization of newcastle disease virus production in t-flask Arifin, Mohd Azmir Salim, Siti Hajar Mel, Maizirwan Abdul Karim, Mohamed Ismail Syed Hassan, Sharifah QR355 Virology In this work, the propagation of Asplin F strain of Newcastle disease virus (NDV) in DF-1 cells was investigated. Experiments were carried out in T-flask to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on virus production. Virus infectivity titre of 6.62 x 107 TCID50/ml was achieved when serum concentration of 0.5% and MOI of 20 was used. 2011-05-17 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/3643/1/Paper_NDV_Azmir.pdf Arifin, Mohd Azmir and Salim, Siti Hajar and Mel, Maizirwan and Abdul Karim, Mohamed Ismail and Syed Hassan, Sharifah (2011) Optimization of newcastle disease virus production in t-flask. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR355 Virology
spellingShingle	QR355 Virology Arifin, Mohd Azmir Salim, Siti Hajar Mel, Maizirwan Abdul Karim, Mohamed Ismail Syed Hassan, Sharifah Optimization of newcastle disease virus production in t-flask
description	In this work, the propagation of Asplin F strain of Newcastle disease virus (NDV) in DF-1 cells was investigated. Experiments were carried out in T-flask to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on virus production. Virus infectivity titre of 6.62 x 107 TCID50/ml was achieved when serum concentration of 0.5% and MOI of 20 was used.
format	Conference or Workshop Item
author	Arifin, Mohd Azmir Salim, Siti Hajar Mel, Maizirwan Abdul Karim, Mohamed Ismail Syed Hassan, Sharifah
author_facet	Arifin, Mohd Azmir Salim, Siti Hajar Mel, Maizirwan Abdul Karim, Mohamed Ismail Syed Hassan, Sharifah
author_sort	Arifin, Mohd Azmir
title	Optimization of newcastle disease virus production in t-flask
title_short	Optimization of newcastle disease virus production in t-flask
title_full	Optimization of newcastle disease virus production in t-flask
title_fullStr	Optimization of newcastle disease virus production in t-flask
title_full_unstemmed	Optimization of newcastle disease virus production in t-flask
title_sort	optimization of newcastle disease virus production in t-flask
publishDate	2011
url	http://irep.iium.edu.my/3643/ http://irep.iium.edu.my/3643/ http://irep.iium.edu.my/3643/1/Paper_NDV_Azmir.pdf
first_indexed	2023-09-18T20:11:32Z
last_indexed	2023-09-18T20:11:32Z
_version_	1777407510790012928

Optimization of newcastle disease virus production in t-flask

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