Mechanisms of α-mangostin-induced antinociception in a rodent model

Objective: Elucidate the antinociceptive mechanisms of a-mangostin isolated from Garcinia malaccensis Linn. Methods: Male mice/rats (n 1⁄4 6/group) were used in this between-group study. To determine a-mangostin’s antinociceptive profile, animals were given a-mangostin orally (3, 30, or 100 mg/kg) 6...

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Main Authors:	Mohd. Sani, Mohd. Hijaz, Bakhtiar, M. Taher, Darnis, Deny Susanti, Lay Kek, Teh, Salleh, Mohd Zaki, Zakaria, Zainul Amiruddin
Format:	Article
Language:	English
Published:	SAGE 2015
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/36495/ http://irep.iium.edu.my/36495/ http://irep.iium.edu.my/36495/ http://irep.iium.edu.my/36495/4/2015-Biol_Res_Nurs.full.pdf

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spelling	iium-364952015-12-12T03:04:23Z http://irep.iium.edu.my/36495/ Mechanisms of α-mangostin-induced antinociception in a rodent model Mohd. Sani, Mohd. Hijaz Bakhtiar, M. Taher Darnis, Deny Susanti Lay Kek, Teh Salleh, Mohd Zaki Zakaria, Zainul Amiruddin RS Pharmacy and materia medica Objective: Elucidate the antinociceptive mechanisms of a-mangostin isolated from Garcinia malaccensis Linn. Methods: Male mice/rats (n 1⁄4 6/group) were used in this between-group study. To determine a-mangostin’s antinociceptive profile, animals were given a-mangostin orally (3, 30, or 100 mg/kg) 60 min before the start of the abdominal constriction or formalin tests. In the hot plate test, the noxious stimulus was applied before and 60, 90, 120, 150, 180, and 210 min after treatment with test solutions. Positive controls received 100 mg/kg acetylsalicylic acid (ASA; oral) or 5 mg/kg morphine (intraperitoneal injection) for the abdominal constriction and hot plate tests, respectively, and either ASA or morphine for the formalin test. Negative controls received vehicle only. To explore a-mangostin’s mechanisms of action, we performed (i) the hot plate test with naloxone (5 mg/kg) pretreatment to verify involvement of opioid receptors; (ii) the abdominal constriction test with 20 mg/kg L-arginine, NG-nitro-L-arginine methyl esters (L-NAME), methylene blue (MB), L-arginine plus L-NAME, or L-arginine plus MB or 10 mg/kg glibenclamide pretreatment to verify involvement of the L-arginine/nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) and Kþ-ATP pathways; and (iii) the paw-licking test using capsaicin (1.6 μg capsaicin/paw), glutamate (10 μmol glutamate/paw), or phorbol 12-myristate 13-acetate (PMA; 0.05 mg/paw) to verify involvement of vanilloid receptors, the glutamatergic system, and protein kinase C (PKC). Results: a-mangostin significantly inhibited nociception (p < .05) in all models. Only naloxone, L- arginine, methylene blue, PMA, and glibenclamide affected a-mangostin antinociception significantly (p < .05). Conclusion: a- mangostin exhibits peripheral and central antinociception through modulation of opioid and vanilloid receptors, the glutamatergic system, and the L-arginine/NO/cGMP/PKC/Kþ-ATP pathways. SAGE 2015-04-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/36495/4/2015-Biol_Res_Nurs.full.pdf Mohd. Sani, Mohd. Hijaz and Bakhtiar, M. Taher and Darnis, Deny Susanti and Lay Kek, Teh and Salleh, Mohd Zaki and Zakaria, Zainul Amiruddin (2015) Mechanisms of α-mangostin-induced antinociception in a rodent model. Biological Research for Nursing, 17 ( 1). pp. 68-77. ISSN 1099-8004 (P), 1552-4175 (O) http://brn.sagepub.com/content/early/2014/04/02/1099800414529648 10.1177/1099800414529648
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Mohd. Sani, Mohd. Hijaz Bakhtiar, M. Taher Darnis, Deny Susanti Lay Kek, Teh Salleh, Mohd Zaki Zakaria, Zainul Amiruddin Mechanisms of α-mangostin-induced antinociception in a rodent model
description	Objective: Elucidate the antinociceptive mechanisms of a-mangostin isolated from Garcinia malaccensis Linn. Methods: Male mice/rats (n 1⁄4 6/group) were used in this between-group study. To determine a-mangostin’s antinociceptive profile, animals were given a-mangostin orally (3, 30, or 100 mg/kg) 60 min before the start of the abdominal constriction or formalin tests. In the hot plate test, the noxious stimulus was applied before and 60, 90, 120, 150, 180, and 210 min after treatment with test solutions. Positive controls received 100 mg/kg acetylsalicylic acid (ASA; oral) or 5 mg/kg morphine (intraperitoneal injection) for the abdominal constriction and hot plate tests, respectively, and either ASA or morphine for the formalin test. Negative controls received vehicle only. To explore a-mangostin’s mechanisms of action, we performed (i) the hot plate test with naloxone (5 mg/kg) pretreatment to verify involvement of opioid receptors; (ii) the abdominal constriction test with 20 mg/kg L-arginine, NG-nitro-L-arginine methyl esters (L-NAME), methylene blue (MB), L-arginine plus L-NAME, or L-arginine plus MB or 10 mg/kg glibenclamide pretreatment to verify involvement of the L-arginine/nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) and Kþ-ATP pathways; and (iii) the paw-licking test using capsaicin (1.6 μg capsaicin/paw), glutamate (10 μmol glutamate/paw), or phorbol 12-myristate 13-acetate (PMA; 0.05 mg/paw) to verify involvement of vanilloid receptors, the glutamatergic system, and protein kinase C (PKC). Results: a-mangostin significantly inhibited nociception (p < .05) in all models. Only naloxone, L- arginine, methylene blue, PMA, and glibenclamide affected a-mangostin antinociception significantly (p < .05). Conclusion: a- mangostin exhibits peripheral and central antinociception through modulation of opioid and vanilloid receptors, the glutamatergic system, and the L-arginine/NO/cGMP/PKC/Kþ-ATP pathways.
format	Article
author	Mohd. Sani, Mohd. Hijaz Bakhtiar, M. Taher Darnis, Deny Susanti Lay Kek, Teh Salleh, Mohd Zaki Zakaria, Zainul Amiruddin
author_facet	Mohd. Sani, Mohd. Hijaz Bakhtiar, M. Taher Darnis, Deny Susanti Lay Kek, Teh Salleh, Mohd Zaki Zakaria, Zainul Amiruddin
author_sort	Mohd. Sani, Mohd. Hijaz
title	Mechanisms of α-mangostin-induced antinociception in a rodent model
title_short	Mechanisms of α-mangostin-induced antinociception in a rodent model
title_full	Mechanisms of α-mangostin-induced antinociception in a rodent model
title_fullStr	Mechanisms of α-mangostin-induced antinociception in a rodent model
title_full_unstemmed	Mechanisms of α-mangostin-induced antinociception in a rodent model
title_sort	mechanisms of α-mangostin-induced antinociception in a rodent model
publisher	SAGE
publishDate	2015
url	http://irep.iium.edu.my/36495/ http://irep.iium.edu.my/36495/ http://irep.iium.edu.my/36495/ http://irep.iium.edu.my/36495/4/2015-Biol_Res_Nurs.full.pdf
first_indexed	2023-09-18T20:52:18Z
last_indexed	2023-09-18T20:52:18Z
_version_	1777410075065843712

Mechanisms of α-mangostin-induced antinociception in a rodent model

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