Pluchea indica extracts stimulates insulin secretion through enhancement of Irs2 and Glut2 genes expression in RIN-5F pancreatic β-cells

Diabetes mellitus (DM) is a chronic metabolic disorder that has reached epidemic proportions with the estimated prevalence rates and trends are escalating largely in developing countries. The awareness of the modern drugs to treat DM which have numerous side effects has led to a recent growing inter...

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Main Authors:	Arief Ichwan, Solachuddin Jauhari, Suriyah, Wastuti Hidayati, Kasmuri, Abdul Razak, Taher, Muhammad
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	RM214 Diet therapy. Diet and dietectics in disease RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/36706/ http://irep.iium.edu.my/36706/ http://irep.iium.edu.my/36706/1/ICNSE_3rd_Kyoto_2014.pdf

id	iium-36706
recordtype	eprints
spelling	iium-367062018-06-19T07:09:05Z http://irep.iium.edu.my/36706/ Pluchea indica extracts stimulates insulin secretion through enhancement of Irs2 and Glut2 genes expression in RIN-5F pancreatic β-cells Arief Ichwan, Solachuddin Jauhari Suriyah, Wastuti Hidayati Kasmuri, Abdul Razak Taher, Muhammad RM214 Diet therapy. Diet and dietectics in disease RS Pharmacy and materia medica Diabetes mellitus (DM) is a chronic metabolic disorder that has reached epidemic proportions with the estimated prevalence rates and trends are escalating largely in developing countries. The awareness of the modern drugs to treat DM which have numerous side effects has led to a recent growing interest in the therapeutic use of natural products, especially those derived from plants. Pluchea indica is abundantly distributed in Malaysia and it is traditionally claimed to have antidiabetic properties. Our previous in vivo study demonstrated that P. indica prevents hyperglycemia in streptozotocin-induced diabetic rats and reduce blood glucose in normal rats. Here we report for the first time the antidiabetic potential of P. indica in the insulin regulation through cell-based in vitro model by using RIN-5F pancreatic β-cells. P. indica was extracted from the powdered dry leaves using soxhlet apparatus with n-hexane, dichloromethane, ethyl acetate and methanol consecutively. The plant also was macerated using water to yield water extract. The maximum tolerable concentration of extracts on RIN-5F pancreatic β-cells was determined by MTT assay. The concentration of 0.2 mg/mL was found to be the maximum concentration of P. indica extracts in the absence of cytotoxicity. The insulin released was measured by a rat insulin enzyme-linked immunoassay (ELISA) system. We found that hexane and water extracts at concentration of 0.05 mg/mL and 0.1 mg/mL respectively stimulated insulin release (p<0.05). Moreover, the insulin stimulation was associated with elevated expression of Insulin receptor substrate 2 (Irs2) and glucose-transporter Glut2 genes as determined by Quantitative Reverse Transcription PCR (qRT-PCR). Taken together, these findings indicate dual positive action of P. indica as a candidate anti-diabetic drug from natural sources by stimulating insulin secretion in β-cells. These findings provide the following insights for future research in exploring the potential clinical usefulness of P. indica for diabetes management. 2014-05-08 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/36706/1/ICNSE_3rd_Kyoto_2014.pdf Arief Ichwan, Solachuddin Jauhari and Suriyah, Wastuti Hidayati and Kasmuri, Abdul Razak and Taher, Muhammad (2014) Pluchea indica extracts stimulates insulin secretion through enhancement of Irs2 and Glut2 genes expression in RIN-5F pancreatic β-cells. In: (ICNSE) International Congress on Natural Sciences and Engineering and (ICCBES) International Congress on Chemical, Biological and Environmental Sciences, 8-9 May 2014, Kyoto, Japan. http://www.iccbes.org/Upload/EditorFiles/201404/20140402171402320.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM214 Diet therapy. Diet and dietectics in disease RS Pharmacy and materia medica
spellingShingle	RM214 Diet therapy. Diet and dietectics in disease RS Pharmacy and materia medica Arief Ichwan, Solachuddin Jauhari Suriyah, Wastuti Hidayati Kasmuri, Abdul Razak Taher, Muhammad Pluchea indica extracts stimulates insulin secretion through enhancement of Irs2 and Glut2 genes expression in RIN-5F pancreatic β-cells
description	Diabetes mellitus (DM) is a chronic metabolic disorder that has reached epidemic proportions with the estimated prevalence rates and trends are escalating largely in developing countries. The awareness of the modern drugs to treat DM which have numerous side effects has led to a recent growing interest in the therapeutic use of natural products, especially those derived from plants. Pluchea indica is abundantly distributed in Malaysia and it is traditionally claimed to have antidiabetic properties. Our previous in vivo study demonstrated that P. indica prevents hyperglycemia in streptozotocin-induced diabetic rats and reduce blood glucose in normal rats. Here we report for the first time the antidiabetic potential of P. indica in the insulin regulation through cell-based in vitro model by using RIN-5F pancreatic β-cells. P. indica was extracted from the powdered dry leaves using soxhlet apparatus with n-hexane, dichloromethane, ethyl acetate and methanol consecutively. The plant also was macerated using water to yield water extract. The maximum tolerable concentration of extracts on RIN-5F pancreatic β-cells was determined by MTT assay. The concentration of 0.2 mg/mL was found to be the maximum concentration of P. indica extracts in the absence of cytotoxicity. The insulin released was measured by a rat insulin enzyme-linked immunoassay (ELISA) system. We found that hexane and water extracts at concentration of 0.05 mg/mL and 0.1 mg/mL respectively stimulated insulin release (p<0.05). Moreover, the insulin stimulation was associated with elevated expression of Insulin receptor substrate 2 (Irs2) and glucose-transporter Glut2 genes as determined by Quantitative Reverse Transcription PCR (qRT-PCR). Taken together, these findings indicate dual positive action of P. indica as a candidate anti-diabetic drug from natural sources by stimulating insulin secretion in β-cells. These findings provide the following insights for future research in exploring the potential clinical usefulness of P. indica for diabetes management.
format	Conference or Workshop Item
author	Arief Ichwan, Solachuddin Jauhari Suriyah, Wastuti Hidayati Kasmuri, Abdul Razak Taher, Muhammad
author_facet	Arief Ichwan, Solachuddin Jauhari Suriyah, Wastuti Hidayati Kasmuri, Abdul Razak Taher, Muhammad
author_sort	Arief Ichwan, Solachuddin Jauhari
title	Pluchea indica extracts stimulates insulin secretion through enhancement of Irs2 and Glut2 genes expression in RIN-5F pancreatic β-cells
title_short	Pluchea indica extracts stimulates insulin secretion through enhancement of Irs2 and Glut2 genes expression in RIN-5F pancreatic β-cells
title_full	Pluchea indica extracts stimulates insulin secretion through enhancement of Irs2 and Glut2 genes expression in RIN-5F pancreatic β-cells
title_fullStr	Pluchea indica extracts stimulates insulin secretion through enhancement of Irs2 and Glut2 genes expression in RIN-5F pancreatic β-cells
title_full_unstemmed	Pluchea indica extracts stimulates insulin secretion through enhancement of Irs2 and Glut2 genes expression in RIN-5F pancreatic β-cells
title_sort	pluchea indica extracts stimulates insulin secretion through enhancement of irs2 and glut2 genes expression in rin-5f pancreatic β-cells
publishDate	2014
url	http://irep.iium.edu.my/36706/ http://irep.iium.edu.my/36706/ http://irep.iium.edu.my/36706/1/ICNSE_3rd_Kyoto_2014.pdf
first_indexed	2023-09-18T20:52:36Z
last_indexed	2023-09-18T20:52:36Z
_version_	1777410094616543232

Pluchea indica extracts stimulates insulin secretion through enhancement of Irs2 and Glut2 genes expression in RIN-5F pancreatic β-cells

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