Water toxicity detection using cell-based hybrid biosensors

This paper presents a novel cell-based biosensor device that contains a quartz crystal microbalance (QCM), integrated with the electric cell-substrate impedance sensing(ECIS) technique. Bovine aortic endothelial cells (BAECs) are cultivated on the sensors and monitored in parallel by both sensors....

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Main Authors:	Fei, Liu, Voiculescu, Ioana, Nordin, Anis Nurashikin, Fang, Li
Format:	Conference or Workshop Item
Language:	English
Published:	2013
Subjects:	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices
Online Access:	http://irep.iium.edu.my/36876/ http://irep.iium.edu.my/36876/ http://irep.iium.edu.my/36876/1/06688374.pdf

id	iium-36876
recordtype	eprints
spelling	iium-368762014-06-09T09:01:53Z http://irep.iium.edu.my/36876/ Water toxicity detection using cell-based hybrid biosensors Fei, Liu Voiculescu, Ioana Nordin, Anis Nurashikin Fang, Li TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices This paper presents a novel cell-based biosensor device that contains a quartz crystal microbalance (QCM), integrated with the electric cell-substrate impedance sensing(ECIS) technique. Bovine aortic endothelial cells (BAECs) are cultivated on the sensors and monitored in parallel by both sensors. When two different sensors monitors the same BAECs monolayer cultures on these sensors the information provided by these sensors is more accurate. Cell culturing microwells were fabricated and glued on this biosensor device for minimizing media and reagent demands and potential automation. The microwells were designed in a manner that can eliminate the fluid shear stress and allow laminar flow for the media and other fluids introduced in the cell-culturing chamber during testing. We demonstrated that BAECS cells could be cultured in these microwells. Resonant frequency and impedance spectroscopy measurements were performed during a time interval. It was demonstrated that this cell- based sensor could be used for toxicity experiments. 2013 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/36876/1/06688374.pdf Fei, Liu and Voiculescu, Ioana and Nordin, Anis Nurashikin and Fang, Li (2013) Water toxicity detection using cell-based hybrid biosensors. In: 2013 IEEE Sensors, 3-6 Nov. 2013, Baltimore, MD, USA. http://ieeexplore.ieee.org/xpl/articleDetails.jsp?arnumber=6688374&searchWithin%3Dwater+toxicity%26punumber%3D6679330%26sortType%3Dasc_p_Sequence%26filter%3DAND%28p_IS_Number%3A6688115%29
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices
spellingShingle	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices Fei, Liu Voiculescu, Ioana Nordin, Anis Nurashikin Fang, Li Water toxicity detection using cell-based hybrid biosensors
description	This paper presents a novel cell-based biosensor device that contains a quartz crystal microbalance (QCM), integrated with the electric cell-substrate impedance sensing(ECIS) technique. Bovine aortic endothelial cells (BAECs) are cultivated on the sensors and monitored in parallel by both sensors. When two different sensors monitors the same BAECs monolayer cultures on these sensors the information provided by these sensors is more accurate. Cell culturing microwells were fabricated and glued on this biosensor device for minimizing media and reagent demands and potential automation. The microwells were designed in a manner that can eliminate the fluid shear stress and allow laminar flow for the media and other fluids introduced in the cell-culturing chamber during testing. We demonstrated that BAECS cells could be cultured in these microwells. Resonant frequency and impedance spectroscopy measurements were performed during a time interval. It was demonstrated that this cell- based sensor could be used for toxicity experiments.
format	Conference or Workshop Item
author	Fei, Liu Voiculescu, Ioana Nordin, Anis Nurashikin Fang, Li
author_facet	Fei, Liu Voiculescu, Ioana Nordin, Anis Nurashikin Fang, Li
author_sort	Fei, Liu
title	Water toxicity detection using cell-based hybrid biosensors
title_short	Water toxicity detection using cell-based hybrid biosensors
title_full	Water toxicity detection using cell-based hybrid biosensors
title_fullStr	Water toxicity detection using cell-based hybrid biosensors
title_full_unstemmed	Water toxicity detection using cell-based hybrid biosensors
title_sort	water toxicity detection using cell-based hybrid biosensors
publishDate	2013
url	http://irep.iium.edu.my/36876/ http://irep.iium.edu.my/36876/ http://irep.iium.edu.my/36876/1/06688374.pdf
first_indexed	2023-09-18T20:52:53Z
last_indexed	2023-09-18T20:52:53Z
_version_	1777410112354254848

Water toxicity detection using cell-based hybrid biosensors

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