Gene expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain

The contributing molecular pathways underlying the pathogenesis of breast cancer need to be better characterized. The principle of our study was to better understand the genetic mechanism of oncogenesis for human breast cancer and to discover new possible tumor markers of use in clinical practice. W...

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Main Authors:	Fouz, Nour, Amid, Azura, Hashim, Yumi Zuhanis Has-Yun
Format:	Article
Language:	English
Published:	Springer 2014
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/36939/ http://irep.iium.edu.my/36939/ http://irep.iium.edu.my/36939/ http://irep.iium.edu.my/36939/1/10.1007_s12010-014-0947-6_%283%29.pdf

id	iium-36939
recordtype	eprints
spelling	iium-369392018-06-19T04:07:19Z http://irep.iium.edu.my/36939/ Gene expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain Fouz, Nour Amid, Azura Hashim, Yumi Zuhanis Has-Yun TP248.13 Biotechnology The contributing molecular pathways underlying the pathogenesis of breast cancer need to be better characterized. The principle of our study was to better understand the genetic mechanism of oncogenesis for human breast cancer and to discover new possible tumor markers of use in clinical practice. We used cDNA microarrays to compare gene expression profiles of treated MCF-7 with recombinant bromelain and untreated MCF-7. SpringGene analysis was carried out of differential expression followed by Ingenuity Pathway Analysis (IPA), to understand the underlying consequence in developing disease and disorders. We identified 1102 known genes differentially expressed to a significant degree (p<0.001) changed between the treatment. Within this gene set, 17 genes were significantly changed between treated cells and the control cells with cutoff fold change more than 1.5. These genes are RBMS1, RPL29, GSTM2, C15orf32, AKT3, BTG1, C6orf62, C7orf60, KIFAP3, FBXO11, ARID4A, COPS2, TBPL1\|SLC2A12, TMEM59, SNORD46, GLTSCR2 and LRRFIP. Our observation on gene expression indicated that recombinant bromelain produces a unique signature affecting different pathways, specific for each congener. The microarray results give a molecular mechanistic insight and functional effects, following recombinant bromelain treatment. The extent of changes in genes related to involved significantly in Gap Junction Signaling, Amyloid processing, Cell Cycle Regulation by BTG Family Proteins and Breast Cancer Regulation by Stathmin1 that play major roles. Springer 2014-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/36939/1/10.1007_s12010-014-0947-6_%283%29.pdf Fouz, Nour and Amid, Azura and Hashim, Yumi Zuhanis Has-Yun (2014) Gene expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain. Applied Biochemistry and Biotechnology, 173 (7). pp. 1618-1639. ISSN 0273-2289 http://link.springer.com/article/10.1007%2Fs12010-014-0947-6 10.1007/s12010-014-0947-6
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Fouz, Nour Amid, Azura Hashim, Yumi Zuhanis Has-Yun Gene expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain
description	The contributing molecular pathways underlying the pathogenesis of breast cancer need to be better characterized. The principle of our study was to better understand the genetic mechanism of oncogenesis for human breast cancer and to discover new possible tumor markers of use in clinical practice. We used cDNA microarrays to compare gene expression profiles of treated MCF-7 with recombinant bromelain and untreated MCF-7. SpringGene analysis was carried out of differential expression followed by Ingenuity Pathway Analysis (IPA), to understand the underlying consequence in developing disease and disorders. We identified 1102 known genes differentially expressed to a significant degree (p<0.001) changed between the treatment. Within this gene set, 17 genes were significantly changed between treated cells and the control cells with cutoff fold change more than 1.5. These genes are RBMS1, RPL29, GSTM2, C15orf32, AKT3, BTG1, C6orf62, C7orf60, KIFAP3, FBXO11, ARID4A, COPS2, TBPL1\|SLC2A12, TMEM59, SNORD46, GLTSCR2 and LRRFIP. Our observation on gene expression indicated that recombinant bromelain produces a unique signature affecting different pathways, specific for each congener. The microarray results give a molecular mechanistic insight and functional effects, following recombinant bromelain treatment. The extent of changes in genes related to involved significantly in Gap Junction Signaling, Amyloid processing, Cell Cycle Regulation by BTG Family Proteins and Breast Cancer Regulation by Stathmin1 that play major roles.
format	Article
author	Fouz, Nour Amid, Azura Hashim, Yumi Zuhanis Has-Yun
author_facet	Fouz, Nour Amid, Azura Hashim, Yumi Zuhanis Has-Yun
author_sort	Fouz, Nour
title	Gene expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain
title_short	Gene expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain
title_full	Gene expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain
title_fullStr	Gene expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain
title_full_unstemmed	Gene expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain
title_sort	gene expression analysis in mcf-7 breast cancer cells treated with recombinant bromelain
publisher	Springer
publishDate	2014
url	http://irep.iium.edu.my/36939/ http://irep.iium.edu.my/36939/ http://irep.iium.edu.my/36939/ http://irep.iium.edu.my/36939/1/10.1007_s12010-014-0947-6_%283%29.pdf
first_indexed	2023-09-18T20:52:59Z
last_indexed	2023-09-18T20:52:59Z
_version_	1777410118165463040

Gene expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain

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