Screening of polyamines in selected Malaysian local plants

Polyamines are an aliphatic compound which has various biological roles in the body. The imbalance of this compound in the body could lead to several diseases such as cancer. Since one of the sources is through diet intake, it is essential to obtain information on polyamines content in the food and...

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Main Authors:	Mohd. Halifah, Nor Fadhlina, Abdul Ghani, Radiah
Format:	Conference or Workshop Item
Language:	English English
Published:	2014
Subjects:	RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/36991/ http://irep.iium.edu.my/36991/1/IRIEE_2014_RADIAH.pdf http://irep.iium.edu.my/36991/4/screening_of_polyamines.pdf

id	iium-36991
recordtype	eprints
spelling	iium-369912015-01-08T10:28:25Z http://irep.iium.edu.my/36991/ Screening of polyamines in selected Malaysian local plants Mohd. Halifah, Nor Fadhlina Abdul Ghani, Radiah RM Therapeutics. Pharmacology Polyamines are an aliphatic compound which has various biological roles in the body. The imbalance of this compound in the body could lead to several diseases such as cancer. Since one of the sources is through diet intake, it is essential to obtain information on polyamines content in the food and natural source products which can be found in fruits, vegetables and herbs. The aim of this study is to extract and quantify the polyamine content in selected Malaysian plants pegaga (Centella asiatica), pecah beling (Strobilanthes crispus), kesum leaves (Polygonum minus), red spinach (Amaranthus gangeticus) and graviola (Annona Muricata) . Then, to classify the classes of polyamines among them. Polyamines were extracted by using perchloric acid based on Smith and Davis (1984) with modification. Then, extracted polyamines from selected samples were dansylated with dansyl chloride before analyzed using HPLC. Putrescine was found in all samples except for fresh C. asiatica. Putrescine was detected in high quantity in fresh P. minus leaves extraction (1526.72nmoles) compared to others. While, the lowest spermidine present among all sample is only in A. muricarata extract juice (0.57 nmoles). Whereas for spermine, fresh S. crispus leaves extraction shown the highest concentration which is 64.99 nmoles followed by fresh P.minus leaves extraction (83.81 nmoles) and fresh A. gangeticus leaves extraction (111.36 nmoles). A. muricata extract juice contain low level of polyamine (6.81 nmoles) followed by fresh C. asiatica leaves extraction which has intermediated level of polyamines which is 163.92 nmoles. Fresh S. crispus leaves extraction (214.72 nmoles), fresh A. gangeticus leaves extraction (403.10 nmoles) and fresh P. minus leaves extraction (1851.69 nmoles) contain high concentration of total polyamines. In conclusion, this study revealed that the polyamines detected in C. asiatica, S. crispus, P. minus , A. gangeticus and A. muricata . Highest total polyamine content is detected in S. crispus followed by A. gangeticus and P. minus,the lowest which falls under low category is detected in A. Muricata juice while the low and intermediate level of polyamines detected in C. asiatica . 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/36991/1/IRIEE_2014_RADIAH.pdf application/pdf en http://irep.iium.edu.my/36991/4/screening_of_polyamines.pdf Mohd. Halifah, Nor Fadhlina and Abdul Ghani, Radiah (2014) Screening of polyamines in selected Malaysian local plants. In: International Research, Invention and Innovation Exhibition 2014 (IRIIE2014), 11th -13th June 2014, Gombak, Kuala Lumpur. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RM Therapeutics. Pharmacology
spellingShingle	RM Therapeutics. Pharmacology Mohd. Halifah, Nor Fadhlina Abdul Ghani, Radiah Screening of polyamines in selected Malaysian local plants
description	Polyamines are an aliphatic compound which has various biological roles in the body. The imbalance of this compound in the body could lead to several diseases such as cancer. Since one of the sources is through diet intake, it is essential to obtain information on polyamines content in the food and natural source products which can be found in fruits, vegetables and herbs. The aim of this study is to extract and quantify the polyamine content in selected Malaysian plants pegaga (Centella asiatica), pecah beling (Strobilanthes crispus), kesum leaves (Polygonum minus), red spinach (Amaranthus gangeticus) and graviola (Annona Muricata) . Then, to classify the classes of polyamines among them. Polyamines were extracted by using perchloric acid based on Smith and Davis (1984) with modification. Then, extracted polyamines from selected samples were dansylated with dansyl chloride before analyzed using HPLC. Putrescine was found in all samples except for fresh C. asiatica. Putrescine was detected in high quantity in fresh P. minus leaves extraction (1526.72nmoles) compared to others. While, the lowest spermidine present among all sample is only in A. muricarata extract juice (0.57 nmoles). Whereas for spermine, fresh S. crispus leaves extraction shown the highest concentration which is 64.99 nmoles followed by fresh P.minus leaves extraction (83.81 nmoles) and fresh A. gangeticus leaves extraction (111.36 nmoles). A. muricata extract juice contain low level of polyamine (6.81 nmoles) followed by fresh C. asiatica leaves extraction which has intermediated level of polyamines which is 163.92 nmoles. Fresh S. crispus leaves extraction (214.72 nmoles), fresh A. gangeticus leaves extraction (403.10 nmoles) and fresh P. minus leaves extraction (1851.69 nmoles) contain high concentration of total polyamines. In conclusion, this study revealed that the polyamines detected in C. asiatica, S. crispus, P. minus , A. gangeticus and A. muricata . Highest total polyamine content is detected in S. crispus followed by A. gangeticus and P. minus,the lowest which falls under low category is detected in A. Muricata juice while the low and intermediate level of polyamines detected in C. asiatica .
format	Conference or Workshop Item
author	Mohd. Halifah, Nor Fadhlina Abdul Ghani, Radiah
author_facet	Mohd. Halifah, Nor Fadhlina Abdul Ghani, Radiah
author_sort	Mohd. Halifah, Nor Fadhlina
title	Screening of polyamines in selected Malaysian local plants
title_short	Screening of polyamines in selected Malaysian local plants
title_full	Screening of polyamines in selected Malaysian local plants
title_fullStr	Screening of polyamines in selected Malaysian local plants
title_full_unstemmed	Screening of polyamines in selected Malaysian local plants
title_sort	screening of polyamines in selected malaysian local plants
publishDate	2014
url	http://irep.iium.edu.my/36991/ http://irep.iium.edu.my/36991/1/IRIEE_2014_RADIAH.pdf http://irep.iium.edu.my/36991/4/screening_of_polyamines.pdf
first_indexed	2023-09-18T20:53:03Z
last_indexed	2023-09-18T20:53:03Z
_version_	1777410122388078592

Screening of polyamines in selected Malaysian local plants

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