Characterization of endothelial cells using electrochemical impedance spectroscopy

In this paper we report on the electrical impedance spectroscopy characterization of endothelial cell lines (RFPEC). For electrical cell-substrate impedance sensing (ECIS) of the endothelial cells a commercially available eight-well cell culture impedance array (ECIS-8W1E) was used. The impedance me...

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Bibliographic Details
Main Authors: Liu, Fei, Arifuzzaman, S. M., Nordin, Anis Nurashikin, Spray, David, Voiculescu, Ioana
Format: Conference or Workshop Item
Language:English
Published: 2010
Subjects:
Online Access:http://irep.iium.edu.my/3702/
http://irep.iium.edu.my/3702/
http://irep.iium.edu.my/3702/1/3.pdf
Description
Summary:In this paper we report on the electrical impedance spectroscopy characterization of endothelial cell lines (RFPEC). For electrical cell-substrate impedance sensing (ECIS) of the endothelial cells a commercially available eight-well cell culture impedance array (ECIS-8W1E) was used. The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cell layer in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements of endothelia cells were also simulated using COMSOL Multiphysics #x2122;, a commercially available modeling package.