Highly cytotoxic agents from luvunga scandens against human breast adenocarcinoma Cell Line (MCF7)

Breast cancer is among of the frequently diagnosed cancer and major of death in the most of country in the world. These led to the necessity for the development of potential chemotherapeutic agents against breast cancer cells with novel mechanisms of actions. The plant-derived triterpeniods commonly...

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Main Authors:	Al-Zikri, Putri Nur Hidayah, Bakhtiar, M. Taher, Darnis, Deny Susanti, Arief Ichwan, Solachuddin Jauhari
Format:	Conference or Workshop Item
Language:	English English English
Published:	2014
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/37030/ http://irep.iium.edu.my/37030/ http://irep.iium.edu.my/37030/2/IRIIE_2014_-_IRIIE_Management_System.pdf http://irep.iium.edu.my/37030/3/Results_for_medal.pdf http://irep.iium.edu.my/37030/8/Jauhari.pdf

id	iium-37030
recordtype	eprints
spelling	iium-370302014-12-09T00:59:15Z http://irep.iium.edu.my/37030/ Highly cytotoxic agents from luvunga scandens against human breast adenocarcinoma Cell Line (MCF7) Al-Zikri, Putri Nur Hidayah Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari RS Pharmacy and materia medica Breast cancer is among of the frequently diagnosed cancer and major of death in the most of country in the world. These led to the necessity for the development of potential chemotherapeutic agents against breast cancer cells with novel mechanisms of actions. The plant-derived triterpeniods commonly used due to its ability to exhibit cytotoxicity against a variety of tumor cells [1]. Luvunga scandens plant (Rutaceae) is one of the plant-derived triterpenoids were responsible to use in this study. In order to investigate the cytotoxicity effect of L. scandens plant, extractions and it isolated compound were derived and assayed on human breast adenocarcinoma cell line (MCF-7). The stems of L. scandens were extracted with n-hexane, dichloromethane (DCM) and ethanol (EtOH). DCM extracts were then further fractionated by vacuum liquid chromatography (VLC) into five extracts; namely n-hexane, n-hexane:DCM, DCM, DCM:MeOH and MeOH. Those five extracts were assayed in vitro cytotoxic activity against MCF-7. Among them, DCM:MeOH fraction was found to be the most effective and was further subjected to isolation procedure and the isolated compound was assayed in vitro cytotoxic activity against MCF-7. Bioactivity-guided fractionation of the fraction through successive column chromatography procedures led to the isolation of a triterpenes-type of compound. A comparison of NMR data showed an appropriate match with the previously reported data. The structure of the compound was elucidated to be lanosta-8, 24-dien-21-oicacid, 3-oxo, (13a, 14b, 17a, 20S)-, a well-known triterpenes with molecular formula C30H46O3. This compound was found to be responsible for the cytotoxicity effects with the half maximal inhibitory concentration (IC50) value 24.8 μM. Whereas IC50 of DCM extract and DCM: MeOH fraction extract is 75 µg/mL and 94 µg/mL respectively. This basic study, therefore, revealed that L. scandens plants are potential sources of cytotoxic agents. 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/37030/2/IRIIE_2014_-_IRIIE_Management_System.pdf application/pdf en http://irep.iium.edu.my/37030/3/Results_for_medal.pdf application/pdf en http://irep.iium.edu.my/37030/8/Jauhari.pdf Al-Zikri, Putri Nur Hidayah and Bakhtiar, M. Taher and Darnis, Deny Susanti and Arief Ichwan, Solachuddin Jauhari (2014) Highly cytotoxic agents from luvunga scandens against human breast adenocarcinoma Cell Line (MCF7). In: International Research, Invention and Innovation Exhibition 2014 (IRIIE2014), 11th -13th June 2014, Cultural Activity Center (CAC), Internatioanal Islamic University Malaysia. http://www.iium.edu.my/irie/14/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Al-Zikri, Putri Nur Hidayah Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Highly cytotoxic agents from luvunga scandens against human breast adenocarcinoma Cell Line (MCF7)
description	Breast cancer is among of the frequently diagnosed cancer and major of death in the most of country in the world. These led to the necessity for the development of potential chemotherapeutic agents against breast cancer cells with novel mechanisms of actions. The plant-derived triterpeniods commonly used due to its ability to exhibit cytotoxicity against a variety of tumor cells [1]. Luvunga scandens plant (Rutaceae) is one of the plant-derived triterpenoids were responsible to use in this study. In order to investigate the cytotoxicity effect of L. scandens plant, extractions and it isolated compound were derived and assayed on human breast adenocarcinoma cell line (MCF-7). The stems of L. scandens were extracted with n-hexane, dichloromethane (DCM) and ethanol (EtOH). DCM extracts were then further fractionated by vacuum liquid chromatography (VLC) into five extracts; namely n-hexane, n-hexane:DCM, DCM, DCM:MeOH and MeOH. Those five extracts were assayed in vitro cytotoxic activity against MCF-7. Among them, DCM:MeOH fraction was found to be the most effective and was further subjected to isolation procedure and the isolated compound was assayed in vitro cytotoxic activity against MCF-7. Bioactivity-guided fractionation of the fraction through successive column chromatography procedures led to the isolation of a triterpenes-type of compound. A comparison of NMR data showed an appropriate match with the previously reported data. The structure of the compound was elucidated to be lanosta-8, 24-dien-21-oicacid, 3-oxo, (13a, 14b, 17a, 20S)-, a well-known triterpenes with molecular formula C30H46O3. This compound was found to be responsible for the cytotoxicity effects with the half maximal inhibitory concentration (IC50) value 24.8 μM. Whereas IC50 of DCM extract and DCM: MeOH fraction extract is 75 µg/mL and 94 µg/mL respectively. This basic study, therefore, revealed that L. scandens plants are potential sources of cytotoxic agents.
format	Conference or Workshop Item
author	Al-Zikri, Putri Nur Hidayah Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari
author_facet	Al-Zikri, Putri Nur Hidayah Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari
author_sort	Al-Zikri, Putri Nur Hidayah
title	Highly cytotoxic agents from luvunga scandens against human breast adenocarcinoma Cell Line (MCF7)
title_short	Highly cytotoxic agents from luvunga scandens against human breast adenocarcinoma Cell Line (MCF7)
title_full	Highly cytotoxic agents from luvunga scandens against human breast adenocarcinoma Cell Line (MCF7)
title_fullStr	Highly cytotoxic agents from luvunga scandens against human breast adenocarcinoma Cell Line (MCF7)
title_full_unstemmed	Highly cytotoxic agents from luvunga scandens against human breast adenocarcinoma Cell Line (MCF7)
title_sort	highly cytotoxic agents from luvunga scandens against human breast adenocarcinoma cell line (mcf7)
publishDate	2014
url	http://irep.iium.edu.my/37030/ http://irep.iium.edu.my/37030/ http://irep.iium.edu.my/37030/2/IRIIE_2014_-_IRIIE_Management_System.pdf http://irep.iium.edu.my/37030/3/Results_for_medal.pdf http://irep.iium.edu.my/37030/8/Jauhari.pdf
first_indexed	2023-09-18T20:53:07Z
last_indexed	2023-09-18T20:53:07Z
_version_	1777410126186020864

Highly cytotoxic agents from luvunga scandens against human breast adenocarcinoma Cell Line (MCF7)

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