Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia

This preliminary study aimed to isolate and identify microbes that inhabit the coelomic fluid of two local species of sea cucumbers collected from Malaysian waters - a ‘gamat’ species i.e. Stichopus chloronotus Brandt, 1835 and the most abundant ‘timun laut’ species in Malaysia i.e. Holothuria (Mert...

Full description

Bibliographic Details
Main Authors:	Lukman, Ahmad Lutfi, Nordin, Noor Faizul Hadry, Kamarudin, Kamarul Rahim
Format:	Article
Language:	English English English
Published:	Penerbit UKM 2014
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/37173/ http://irep.iium.edu.my/37173/ http://irep.iium.edu.my/37173/1/2014_Sains_Malaysiana_Ahmad_Lutfi_et_al.pdf http://irep.iium.edu.my/37173/4/37173_Microbial%20population%20in%20the%20coelomic%20fluid%20of%20stichopus%20chloronotus%20and%20holothuria_SCOPUS.pdf http://irep.iium.edu.my/37173/5/37173_Microbial%20population%20in%20the%20coelomic%20fluid%20of%20stichopus%20chloronotus%20and%20holothuria_WoS.pdf

id	iium-37173
recordtype	eprints
spelling	iium-371732018-06-19T06:29:54Z http://irep.iium.edu.my/37173/ Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia Lukman, Ahmad Lutfi Nordin, Noor Faizul Hadry Kamarudin, Kamarul Rahim QR Microbiology This preliminary study aimed to isolate and identify microbes that inhabit the coelomic fluid of two local species of sea cucumbers collected from Malaysian waters - a ‘gamat’ species i.e. Stichopus chloronotus Brandt, 1835 and the most abundant ‘timun laut’ species in Malaysia i.e. Holothuria (Mertensiothuria) leucospilota (Brandt 1835). Phylogenetic analyses of partial 16S rRNA mtDNA gene sequences suggested the presence of at least eight microbial genera i.e. five bacterial genera - Bacillus, Exiguobacterium, Pseudomonas, Stenotrophomonas and Vibrio - isolated from the coelomic fluid of H. leucospilota and three genera of gram-positive bacteria from the Micrococcaceae family - Kytococcus, Micrococcus and either Kocuria or Rothia - isolated from the coelomic fluid of S. chloronotus. We speculate that less diverse microbial population in S. chloronotus as compared to H. leucospilota could be due to a number of environmental factors e.g. penetration of light surrounding the habitats of both species, the feeding behaviour of H. leucospilota and the higher level of antimicrobial properties of coelomic fluid in S. chloronotus. In terms of antimicrobial-resistance capability test, an isolate from genus Pseudomonas that is suspected to be P. alcaligenes exhibited high resistance towards streptomycin. Another isolate from genus Stenotrophomonas that was suspected to be S. maltophilia showed moderate resistance towards streptomycin and lower resistance towards kanamycin. Both isolates were from the coelomic fluid of H. leucospilota. Tetracycline inhibited the growth of all bacterial isolates tested. Further studies with more specimens of S. choronotus and H. leucospilota from broader geographical locations and the use of complete mtDNA genes along with morphological approaches for species identification may facilitate to provide better insights into the microbial population in the coelomic fluid of both local sea cucumber species. Penerbit UKM 2014-07-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/37173/1/2014_Sains_Malaysiana_Ahmad_Lutfi_et_al.pdf application/pdf en http://irep.iium.edu.my/37173/4/37173_Microbial%20population%20in%20the%20coelomic%20fluid%20of%20stichopus%20chloronotus%20and%20holothuria_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/37173/5/37173_Microbial%20population%20in%20the%20coelomic%20fluid%20of%20stichopus%20chloronotus%20and%20holothuria_WoS.pdf Lukman, Ahmad Lutfi and Nordin, Noor Faizul Hadry and Kamarudin, Kamarul Rahim (2014) Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia. Sains Malaysiana, 43 (7). pp. 1013-1021. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid43bil7_2014/KandunganJilid43Bil7_2014.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	QR Microbiology
spellingShingle	QR Microbiology Lukman, Ahmad Lutfi Nordin, Noor Faizul Hadry Kamarudin, Kamarul Rahim Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia
description	This preliminary study aimed to isolate and identify microbes that inhabit the coelomic fluid of two local species of sea cucumbers collected from Malaysian waters - a ‘gamat’ species i.e. Stichopus chloronotus Brandt, 1835 and the most abundant ‘timun laut’ species in Malaysia i.e. Holothuria (Mertensiothuria) leucospilota (Brandt 1835). Phylogenetic analyses of partial 16S rRNA mtDNA gene sequences suggested the presence of at least eight microbial genera i.e. five bacterial genera - Bacillus, Exiguobacterium, Pseudomonas, Stenotrophomonas and Vibrio - isolated from the coelomic fluid of H. leucospilota and three genera of gram-positive bacteria from the Micrococcaceae family - Kytococcus, Micrococcus and either Kocuria or Rothia - isolated from the coelomic fluid of S. chloronotus. We speculate that less diverse microbial population in S. chloronotus as compared to H. leucospilota could be due to a number of environmental factors e.g. penetration of light surrounding the habitats of both species, the feeding behaviour of H. leucospilota and the higher level of antimicrobial properties of coelomic fluid in S. chloronotus. In terms of antimicrobial-resistance capability test, an isolate from genus Pseudomonas that is suspected to be P. alcaligenes exhibited high resistance towards streptomycin. Another isolate from genus Stenotrophomonas that was suspected to be S. maltophilia showed moderate resistance towards streptomycin and lower resistance towards kanamycin. Both isolates were from the coelomic fluid of H. leucospilota. Tetracycline inhibited the growth of all bacterial isolates tested. Further studies with more specimens of S. choronotus and H. leucospilota from broader geographical locations and the use of complete mtDNA genes along with morphological approaches for species identification may facilitate to provide better insights into the microbial population in the coelomic fluid of both local sea cucumber species.
format	Article
author	Lukman, Ahmad Lutfi Nordin, Noor Faizul Hadry Kamarudin, Kamarul Rahim
author_facet	Lukman, Ahmad Lutfi Nordin, Noor Faizul Hadry Kamarudin, Kamarul Rahim
author_sort	Lukman, Ahmad Lutfi
title	Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia
title_short	Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia
title_full	Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia
title_fullStr	Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia
title_full_unstemmed	Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia
title_sort	microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from malaysian waters = populasi mikrob dalam cecair selom stichopus chloronotus dan holothuria (mertensiothuria) leucospilota dari perairan malaysia
publisher	Penerbit UKM
publishDate	2014
url	http://irep.iium.edu.my/37173/ http://irep.iium.edu.my/37173/ http://irep.iium.edu.my/37173/1/2014_Sains_Malaysiana_Ahmad_Lutfi_et_al.pdf http://irep.iium.edu.my/37173/4/37173_Microbial%20population%20in%20the%20coelomic%20fluid%20of%20stichopus%20chloronotus%20and%20holothuria_SCOPUS.pdf http://irep.iium.edu.my/37173/5/37173_Microbial%20population%20in%20the%20coelomic%20fluid%20of%20stichopus%20chloronotus%20and%20holothuria_WoS.pdf
first_indexed	2023-09-18T20:53:20Z
last_indexed	2023-09-18T20:53:20Z
_version_	1777410140322922496

Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia

Similar Items