Isolation and pharmacological characterization of a short neurotoxin, α-elapitoxin-Bf1b (α-EPTX-Bf1b) from Malaysian Bungarus fasciatus

Introduction: In Malaysia and other countries in South East Asia, kraits (genus Bungarus) are considered to be a medically important species. The Golden banded krait (Bungarus fasciatus) is one species found in Malaysia. Like many other krait species, Bungarus fasciatus venom has previously been rep...

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Main Authors:	Ahmad Rusmili, Muhamad Rusdi, Mustafa, Mohd Rais, Othman, Iekhsan, Hodgson, Wayne C.
Format:	Conference or Workshop Item
Language:	English English
Published:	2013
Subjects:	RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/37204/ http://irep.iium.edu.my/37204/1/alpha-EPTX0BF1b_Brazil_4.pdf http://irep.iium.edu.my/37204/3/Oral_presenter.pdf

id	iium-37204
recordtype	eprints
spelling	iium-372042014-07-09T04:44:17Z http://irep.iium.edu.my/37204/ Isolation and pharmacological characterization of a short neurotoxin, α-elapitoxin-Bf1b (α-EPTX-Bf1b) from Malaysian Bungarus fasciatus Ahmad Rusmili, Muhamad Rusdi Mustafa, Mohd Rais Othman, Iekhsan Hodgson, Wayne C. RM Therapeutics. Pharmacology Introduction: In Malaysia and other countries in South East Asia, kraits (genus Bungarus) are considered to be a medically important species. The Golden banded krait (Bungarus fasciatus) is one species found in Malaysia. Like many other krait species, Bungarus fasciatus venom has previously been reported to contain a number of postsynaptic neurotoxins that cause blockade of neuronal transmission involving the nicotinic acetylcholine receptor. However, not many of these toxins have been isolated and fully characterized. Here, we would describe the isolation and pharmacological characterization of a novel short neurotoxin that we named α- elapidtoxin-Bf1b. Methods and Results: The toxin was purified by a combination of gel filtration, cation-exchange and reverse-phase chromatography. Based on mass spectrometry and sequencing data, the toxin showed high homology with another toxin, Neurotoxin 3FTx-RI that has previously only been detected in the Bungarus fasciatus venom gland transcriptome. The toxin has an approximate molecular mass of 6.9 kDa and caused concentration-dependent and time-dependent neuromuscular blockade when tested in the indirectly-stimulated chick biventer cervicis muscle preparation. The toxin is also partially reversible after continuous washing. Monovalent Bungarus fasciatus and neurotoxic polyvalent antivenoms were found to be ineffective in reversing the neurotoxicity effect in indirectly-stimulated chick biventer cervicis preparation. Conclusion: We have isolated a novel short neurotoxin from Malaysian Bungarus fasciatus. This toxin is perhaps the first short neurotoxin from Bungarus fasciatus that has been reported to be resistant to both monovalent Bungarus fasciatus and neurotoxic polyvalent antivenoms. 2013 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/37204/1/alpha-EPTX0BF1b_Brazil_4.pdf application/pdf en http://irep.iium.edu.my/37204/3/Oral_presenter.pdf Ahmad Rusmili, Muhamad Rusdi and Mustafa, Mohd Rais and Othman, Iekhsan and Hodgson, Wayne C. (2013) Isolation and pharmacological characterization of a short neurotoxin, α-elapitoxin-Bf1b (α-EPTX-Bf1b) from Malaysian Bungarus fasciatus. In: XI Congress of the Pan American Section of the International Society on Toxinology and the XII Congress of the Brazilian Society of Toxinology., 8-13 November 2013, Guaruja, Sao Paolo, Brazil. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RM Therapeutics. Pharmacology
spellingShingle	RM Therapeutics. Pharmacology Ahmad Rusmili, Muhamad Rusdi Mustafa, Mohd Rais Othman, Iekhsan Hodgson, Wayne C. Isolation and pharmacological characterization of a short neurotoxin, α-elapitoxin-Bf1b (α-EPTX-Bf1b) from Malaysian Bungarus fasciatus
description	Introduction: In Malaysia and other countries in South East Asia, kraits (genus Bungarus) are considered to be a medically important species. The Golden banded krait (Bungarus fasciatus) is one species found in Malaysia. Like many other krait species, Bungarus fasciatus venom has previously been reported to contain a number of postsynaptic neurotoxins that cause blockade of neuronal transmission involving the nicotinic acetylcholine receptor. However, not many of these toxins have been isolated and fully characterized. Here, we would describe the isolation and pharmacological characterization of a novel short neurotoxin that we named α- elapidtoxin-Bf1b. Methods and Results: The toxin was purified by a combination of gel filtration, cation-exchange and reverse-phase chromatography. Based on mass spectrometry and sequencing data, the toxin showed high homology with another toxin, Neurotoxin 3FTx-RI that has previously only been detected in the Bungarus fasciatus venom gland transcriptome. The toxin has an approximate molecular mass of 6.9 kDa and caused concentration-dependent and time-dependent neuromuscular blockade when tested in the indirectly-stimulated chick biventer cervicis muscle preparation. The toxin is also partially reversible after continuous washing. Monovalent Bungarus fasciatus and neurotoxic polyvalent antivenoms were found to be ineffective in reversing the neurotoxicity effect in indirectly-stimulated chick biventer cervicis preparation. Conclusion: We have isolated a novel short neurotoxin from Malaysian Bungarus fasciatus. This toxin is perhaps the first short neurotoxin from Bungarus fasciatus that has been reported to be resistant to both monovalent Bungarus fasciatus and neurotoxic polyvalent antivenoms.
format	Conference or Workshop Item
author	Ahmad Rusmili, Muhamad Rusdi Mustafa, Mohd Rais Othman, Iekhsan Hodgson, Wayne C.
author_facet	Ahmad Rusmili, Muhamad Rusdi Mustafa, Mohd Rais Othman, Iekhsan Hodgson, Wayne C.
author_sort	Ahmad Rusmili, Muhamad Rusdi
title	Isolation and pharmacological characterization of a short neurotoxin, α-elapitoxin-Bf1b (α-EPTX-Bf1b) from Malaysian Bungarus fasciatus
title_short	Isolation and pharmacological characterization of a short neurotoxin, α-elapitoxin-Bf1b (α-EPTX-Bf1b) from Malaysian Bungarus fasciatus
title_full	Isolation and pharmacological characterization of a short neurotoxin, α-elapitoxin-Bf1b (α-EPTX-Bf1b) from Malaysian Bungarus fasciatus
title_fullStr	Isolation and pharmacological characterization of a short neurotoxin, α-elapitoxin-Bf1b (α-EPTX-Bf1b) from Malaysian Bungarus fasciatus
title_full_unstemmed	Isolation and pharmacological characterization of a short neurotoxin, α-elapitoxin-Bf1b (α-EPTX-Bf1b) from Malaysian Bungarus fasciatus
title_sort	isolation and pharmacological characterization of a short neurotoxin, α-elapitoxin-bf1b (α-eptx-bf1b) from malaysian bungarus fasciatus
publishDate	2013
url	http://irep.iium.edu.my/37204/ http://irep.iium.edu.my/37204/1/alpha-EPTX0BF1b_Brazil_4.pdf http://irep.iium.edu.my/37204/3/Oral_presenter.pdf
first_indexed	2023-09-18T20:53:21Z
last_indexed	2023-09-18T20:53:21Z
_version_	1777410141773103104

Isolation and pharmacological characterization of a short neurotoxin, α-elapitoxin-Bf1b (α-EPTX-Bf1b) from Malaysian Bungarus fasciatus

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