The growth study of vero cells in different type of microcarrier
The fact of microcarrier (MC) culture introduces new possibilities and makes possible the practical high-yield culture of anchorage-dependent cells has generated a considerable focus in this study. The objective of this research was to study the comparison of Vero cell growth on different types of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Scientific Research
2010
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/3721/ http://irep.iium.edu.my/3721/ http://irep.iium.edu.my/3721/ http://irep.iium.edu.my/3721/1/MSA-Microcarrier_2010.pdf |
| Summary: | The fact of microcarrier (MC) culture introduces new possibilities and makes possible the practical high-yield culture
of anchorage-dependent cells has generated a considerable focus in this study. The objective of this research was to
study the comparison of Vero cell growth on different types of commercial microcarriers; Cytodex-1, Cytodex-3,
Hillex® II and Plastic Plus in spinner vessel and two liters bioreactor cultured for 96 hours. Biological performance of
the microcarrier in RPMI media showed the preference of Vero cell grew on Cytodex 3 microcarriers with highest
maximum viable cell number (2.4 × 105 cells/ml) followed by Cytodex 1, Hillex and Plustic Plus. Vero cell on Cytodex-
3 data in spinner flask was compared in bioreactor and result showed higher viable cell number in biorector. Thus,
this dextran-crosslink gelatin microcarrier (Cytodex 3) provided the best surface for cell attachment and fast proliferation.
At the end of this cell growth improvement will be used for virus transfection producing a vaccine in bioreactor. |
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