Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells

Electric cell-substrate impedance sensing (ECIS) method can be used as a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. Changes in impedance of the cells due to growth and attachment can be modeled as an equivalent circuit consisting of resistors an...

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Main Authors:	Siddiquei, H. R., Nordin, Anis Nurashikin, Ibrahimy, Muhammad Ibn, Arifin, Mohd Azmir, Sulong, Nurul Hafizah, Mel, Maizirwan, Voiculescu, Ioana
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	TK7885 Computer engineering
Online Access:	http://irep.iium.edu.my/3733/ http://irep.iium.edu.my/3733/ http://irep.iium.edu.my/3733/1/30.pdf

id	iium-3733
recordtype	eprints
spelling	iium-37332011-11-13T08:23:43Z http://irep.iium.edu.my/3733/ Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells Siddiquei, H. R. Nordin, Anis Nurashikin Ibrahimy, Muhammad Ibn Arifin, Mohd Azmir Sulong, Nurul Hafizah Mel, Maizirwan Voiculescu, Ioana TK7885 Computer engineering Electric cell-substrate impedance sensing (ECIS) method can be used as a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. Changes in impedance of the cells due to growth and attachment can be modeled as an equivalent circuit consisting of resistors and capacitors of both the cell culture media and the cells. In this work, a biosensor which measures the impedance in DF-1 cells (derived from chicken embroyonic fibroblasts and CEF cells) are presented. The biosensor consists of a Teflon cell holder and two gold electrodes. Experimental measurements were conducted using DF-1 cells cultured in DMEM media. Two different experiments were conducted namely; the control experiment (holder contains only DMEM media) and the cell experiment (holder contains both DMEM media and cells). The biosensor was placed in an incubator with optimum settings for cell growth. Impedance measurements were sampled at six hour intervals. Based on these measurements the resistance and capacitance change due to the growth of the DF-1 cells were calculated. It was observed that significant change in resistance and capacitance values occurs in the first six hours, where cell growth and attachment is most active. After this period of time, the cells become confluent and capacitance values become saturated. 2010-05 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/3733/1/30.pdf Siddiquei, H. R. and Nordin, Anis Nurashikin and Ibrahimy, Muhammad Ibn and Arifin, Mohd Azmir and Sulong, Nurul Hafizah and Mel, Maizirwan and Voiculescu, Ioana (2010) Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells. In: International Conference on Computer and Communication Engineering (ICCCE 2010), 11-13 May 2010, Kuala Lumpur. http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=5556772&tag=1
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TK7885 Computer engineering
spellingShingle	TK7885 Computer engineering Siddiquei, H. R. Nordin, Anis Nurashikin Ibrahimy, Muhammad Ibn Arifin, Mohd Azmir Sulong, Nurul Hafizah Mel, Maizirwan Voiculescu, Ioana Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells
description	Electric cell-substrate impedance sensing (ECIS) method can be used as a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. Changes in impedance of the cells due to growth and attachment can be modeled as an equivalent circuit consisting of resistors and capacitors of both the cell culture media and the cells. In this work, a biosensor which measures the impedance in DF-1 cells (derived from chicken embroyonic fibroblasts and CEF cells) are presented. The biosensor consists of a Teflon cell holder and two gold electrodes. Experimental measurements were conducted using DF-1 cells cultured in DMEM media. Two different experiments were conducted namely; the control experiment (holder contains only DMEM media) and the cell experiment (holder contains both DMEM media and cells). The biosensor was placed in an incubator with optimum settings for cell growth. Impedance measurements were sampled at six hour intervals. Based on these measurements the resistance and capacitance change due to the growth of the DF-1 cells were calculated. It was observed that significant change in resistance and capacitance values occurs in the first six hours, where cell growth and attachment is most active. After this period of time, the cells become confluent and capacitance values become saturated.
format	Conference or Workshop Item
author	Siddiquei, H. R. Nordin, Anis Nurashikin Ibrahimy, Muhammad Ibn Arifin, Mohd Azmir Sulong, Nurul Hafizah Mel, Maizirwan Voiculescu, Ioana
author_facet	Siddiquei, H. R. Nordin, Anis Nurashikin Ibrahimy, Muhammad Ibn Arifin, Mohd Azmir Sulong, Nurul Hafizah Mel, Maizirwan Voiculescu, Ioana
author_sort	Siddiquei, H. R.
title	Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells
title_short	Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells
title_full	Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells
title_fullStr	Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells
title_full_unstemmed	Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells
title_sort	electrical cell-substrate impedance sensing (ecis) based biosensor for characterization of df-1 cells
publishDate	2010
url	http://irep.iium.edu.my/3733/ http://irep.iium.edu.my/3733/ http://irep.iium.edu.my/3733/1/30.pdf
first_indexed	2023-09-18T20:11:41Z
last_indexed	2023-09-18T20:11:41Z
_version_	1777407519529893888

Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells

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