GC-MS based metabolic profiling of ginger (Z. officinale Rosc.) plantlets

Introduction and Objective: Ginger (Zingiber officinale Rosc.) is a valuable plant because of wide application in culinary and medicinal uses. Major pungent constituents of ginger rhizomes namely gingerols and their derivatives such as shogaols and paradols are believed to be responsible for most of...

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Main Authors:	Shapiei, Shazatul Azida, Jameel, Mahdi Harith, Budi Muljono, Julia Retno Andayani
Format:	Conference or Workshop Item
Language:	English
Published:	2012
Subjects:	Q Science (General) QD Chemistry T Technology (General)
Online Access:	http://irep.iium.edu.my/37372/ http://irep.iium.edu.my/37372/ http://irep.iium.edu.my/37372/1/NAPRO2012.pdf

id	iium-37372
recordtype	eprints
spelling	iium-373722014-08-08T06:52:09Z http://irep.iium.edu.my/37372/ GC-MS based metabolic profiling of ginger (Z. officinale Rosc.) plantlets Shapiei, Shazatul Azida Jameel, Mahdi Harith Budi Muljono, Julia Retno Andayani Q Science (General) QD Chemistry T Technology (General) Introduction and Objective: Ginger (Zingiber officinale Rosc.) is a valuable plant because of wide application in culinary and medicinal uses. Major pungent constituents of ginger rhizomes namely gingerols and their derivatives such as shogaols and paradols are believed to be responsible for most of their pharmacological activities. The proportion of these compounds might vary considerably due to either environmental or genetic factors. In the present study, we intended to do profiling ginger root metabolites of tissue culture-derived plantlets originated from Bukit Tinggi and Sabah using gas chromatography-mass spectrometry. Method: Sample preparation and MSTFA derivatization of chloroform fraction of lyophilized ginger root were carried out according to Shepherd's method (5) with a slight modification. The GC-MS data were obtained on a Agilent gas chromatograph (6890 N) with a non polar column HP-5MS 5% phenyl methyl siloxane (30 m x 0.25 mm i.d.), film thickness 0.25 µm and interfaced with Mass Detector (MSD). The GC oven and transfer line temperature, 250C; carrier gas helium, (1.2 ml/min), ionization energy, 70 eV. Oven temperature programmed started at 50C for 5 min, to 250 C at 10 C/min till the end of analysis. The peak identity was checked using the Wiley7n.1 spectra data based library as well as NIST Web book. Results: Sequiterpenes hydrocarbon such as -zingiberene, ar-curcumene, -farnacene and -sesquiphellandrene were obtained in the gingerol root plantlets. Te same chemicals are reported to be the most abundance essential oils of gingr rhizomes. In addition a considerable amount of paradola gingerol related compound was also found in the ginger root together with cis-6-shogaol. On the contrary, major constituents of fresh rhizome, gingerol was only found in a trace amount presence in both ginger root plantlets. A similar GC-MS profile of terpenoids and gingerol-relative compounds were detected using a non polar capillary column HP-5MS following a MSTFA derivatization of the analytes. Conclusion: Ginger root plantlets contain a sesquiterpenes -zingiberene and paradol (shogaol related compound) as their major constituents. Apparently, the same artificial growth conditions such as media composition, light intensity and growth temperature applied on giger have resulted in a small vaiability of the ginger metabolites. 2012-11-28 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/37372/1/NAPRO2012.pdf Shapiei, Shazatul Azida and Jameel, Mahdi Harith and Budi Muljono, Julia Retno Andayani (2012) GC-MS based metabolic profiling of ginger (Z. officinale Rosc.) plantlets. In: 4th International Conference on National Products for Health and Beauty (NAPRO 4), 28-30 Nov. 2012, Chiang Mai, Thailand. http://www.natpro4-2012.cmu.ac.th/status.php
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) QD Chemistry T Technology (General)
spellingShingle	Q Science (General) QD Chemistry T Technology (General) Shapiei, Shazatul Azida Jameel, Mahdi Harith Budi Muljono, Julia Retno Andayani GC-MS based metabolic profiling of ginger (Z. officinale Rosc.) plantlets
description	Introduction and Objective: Ginger (Zingiber officinale Rosc.) is a valuable plant because of wide application in culinary and medicinal uses. Major pungent constituents of ginger rhizomes namely gingerols and their derivatives such as shogaols and paradols are believed to be responsible for most of their pharmacological activities. The proportion of these compounds might vary considerably due to either environmental or genetic factors. In the present study, we intended to do profiling ginger root metabolites of tissue culture-derived plantlets originated from Bukit Tinggi and Sabah using gas chromatography-mass spectrometry. Method: Sample preparation and MSTFA derivatization of chloroform fraction of lyophilized ginger root were carried out according to Shepherd's method (5) with a slight modification. The GC-MS data were obtained on a Agilent gas chromatograph (6890 N) with a non polar column HP-5MS 5% phenyl methyl siloxane (30 m x 0.25 mm i.d.), film thickness 0.25 µm and interfaced with Mass Detector (MSD). The GC oven and transfer line temperature, 250C; carrier gas helium, (1.2 ml/min), ionization energy, 70 eV. Oven temperature programmed started at 50C for 5 min, to 250 C at 10 C/min till the end of analysis. The peak identity was checked using the Wiley7n.1 spectra data based library as well as NIST Web book. Results: Sequiterpenes hydrocarbon such as -zingiberene, ar-curcumene, -farnacene and -sesquiphellandrene were obtained in the gingerol root plantlets. Te same chemicals are reported to be the most abundance essential oils of gingr rhizomes. In addition a considerable amount of paradola gingerol related compound was also found in the ginger root together with cis-6-shogaol. On the contrary, major constituents of fresh rhizome, gingerol was only found in a trace amount presence in both ginger root plantlets. A similar GC-MS profile of terpenoids and gingerol-relative compounds were detected using a non polar capillary column HP-5MS following a MSTFA derivatization of the analytes. Conclusion: Ginger root plantlets contain a sesquiterpenes -zingiberene and paradol (shogaol related compound) as their major constituents. Apparently, the same artificial growth conditions such as media composition, light intensity and growth temperature applied on giger have resulted in a small vaiability of the ginger metabolites.
format	Conference or Workshop Item
author	Shapiei, Shazatul Azida Jameel, Mahdi Harith Budi Muljono, Julia Retno Andayani
author_facet	Shapiei, Shazatul Azida Jameel, Mahdi Harith Budi Muljono, Julia Retno Andayani
author_sort	Shapiei, Shazatul Azida
title	GC-MS based metabolic profiling of ginger (Z. officinale Rosc.) plantlets
title_short	GC-MS based metabolic profiling of ginger (Z. officinale Rosc.) plantlets
title_full	GC-MS based metabolic profiling of ginger (Z. officinale Rosc.) plantlets
title_fullStr	GC-MS based metabolic profiling of ginger (Z. officinale Rosc.) plantlets
title_full_unstemmed	GC-MS based metabolic profiling of ginger (Z. officinale Rosc.) plantlets
title_sort	gc-ms based metabolic profiling of ginger (z. officinale rosc.) plantlets
publishDate	2012
url	http://irep.iium.edu.my/37372/ http://irep.iium.edu.my/37372/ http://irep.iium.edu.my/37372/1/NAPRO2012.pdf
first_indexed	2023-09-18T20:53:38Z
last_indexed	2023-09-18T20:53:38Z
_version_	1777410158835531776

GC-MS based metabolic profiling of ginger (Z. officinale Rosc.) plantlets

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