Statistical optimization of fermentation conditions for cellulase production from palm oil mill effluent

Palm oil mill effluent discharged by the oil palm industries is considered as the mixed of high polluted effluent which is abundant (about 20 million tonnes year-1) and its effect contributes to the serious environmental problems through the pollution of water bodies. Approach: The aim of this st...

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Main Authors:	Jamal Ibrahim, Daoud, Alam, Md. Zahangir
Format:	Article
Language:	English
Published:	Science Publications 2010
Subjects:	TD Environmental technology. Sanitary engineering
Online Access:	http://irep.iium.edu.my/3745/ http://irep.iium.edu.my/3745/ http://irep.iium.edu.my/3745/1/ajessp.2010.66.70.pdf

id	iium-3745
recordtype	eprints
spelling	iium-37452011-09-20T05:54:37Z http://irep.iium.edu.my/3745/ Statistical optimization of fermentation conditions for cellulase production from palm oil mill effluent Jamal Ibrahim, Daoud Alam, Md. Zahangir TD Environmental technology. Sanitary engineering Palm oil mill effluent discharged by the oil palm industries is considered as the mixed of high polluted effluent which is abundant (about 20 million tonnes year-1) and its effect contributes to the serious environmental problems through the pollution of water bodies. Approach: The aim of this study was to identify the potential of low cost substrate such as Palm Oil Mill Effluent (POME) for the production of cellulase enzyme by liquid state bioconversion. The filamentous fungus Trichoderma harzianum was used for liquid state bioconversion of POME for cellulase production. Statistical optimization was carried out to evaluate the physico-chemical parameters (factors) for maximum cellulase production by 2-level fractional factorial design with six central points. The polynomial regression model was developed using the experimental data including the effects of linear, quadratic and interaction of the factors. The factors involved were substrate (POME) and co-substrate (wheat flour) concentrations, temperature, pH, inoculum and agitation. Results: Statistical analysis showed that the optimum conditions were: Temperature of 30°C, substrate concentration of 2%, wheat flour concentration of 3%, pH of 4, inoculum of 3% and agitation of 200 rpm. Under these conditions, the model predicted the enzyme production to be about 14 FPU mL-1. Analysis Of Variance (ANOVA) of the design showed a high coefficient of determination (R2) value of 0.999, thus ensuring a high satisfactory adjustment of the quadratic model with the experimental data. Conclusion/Recommendations: This study indicates a better solution for waste management through the utilization of POME for cellulase production that could be used in the industrial applications such as bioethanol production. Science Publications 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/3745/1/ajessp.2010.66.70.pdf Jamal Ibrahim, Daoud and Alam, Md. Zahangir (2010) Statistical optimization of fermentation conditions for cellulase production from palm oil mill effluent. American Journal of Environmental Sciences, 6 (1). pp. 66-70. ISSN 1553-345X http://thescipub.com/abstract/10.3844/ajessp.2010.66.70
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TD Environmental technology. Sanitary engineering
spellingShingle	TD Environmental technology. Sanitary engineering Jamal Ibrahim, Daoud Alam, Md. Zahangir Statistical optimization of fermentation conditions for cellulase production from palm oil mill effluent
description	Palm oil mill effluent discharged by the oil palm industries is considered as the mixed of high polluted effluent which is abundant (about 20 million tonnes year-1) and its effect contributes to the serious environmental problems through the pollution of water bodies. Approach: The aim of this study was to identify the potential of low cost substrate such as Palm Oil Mill Effluent (POME) for the production of cellulase enzyme by liquid state bioconversion. The filamentous fungus Trichoderma harzianum was used for liquid state bioconversion of POME for cellulase production. Statistical optimization was carried out to evaluate the physico-chemical parameters (factors) for maximum cellulase production by 2-level fractional factorial design with six central points. The polynomial regression model was developed using the experimental data including the effects of linear, quadratic and interaction of the factors. The factors involved were substrate (POME) and co-substrate (wheat flour) concentrations, temperature, pH, inoculum and agitation. Results: Statistical analysis showed that the optimum conditions were: Temperature of 30°C, substrate concentration of 2%, wheat flour concentration of 3%, pH of 4, inoculum of 3% and agitation of 200 rpm. Under these conditions, the model predicted the enzyme production to be about 14 FPU mL-1. Analysis Of Variance (ANOVA) of the design showed a high coefficient of determination (R2) value of 0.999, thus ensuring a high satisfactory adjustment of the quadratic model with the experimental data. Conclusion/Recommendations: This study indicates a better solution for waste management through the utilization of POME for cellulase production that could be used in the industrial applications such as bioethanol production.
format	Article
author	Jamal Ibrahim, Daoud Alam, Md. Zahangir
author_facet	Jamal Ibrahim, Daoud Alam, Md. Zahangir
author_sort	Jamal Ibrahim, Daoud
title	Statistical optimization of fermentation conditions for cellulase production from palm oil mill effluent
title_short	Statistical optimization of fermentation conditions for cellulase production from palm oil mill effluent
title_full	Statistical optimization of fermentation conditions for cellulase production from palm oil mill effluent
title_fullStr	Statistical optimization of fermentation conditions for cellulase production from palm oil mill effluent
title_full_unstemmed	Statistical optimization of fermentation conditions for cellulase production from palm oil mill effluent
title_sort	statistical optimization of fermentation conditions for cellulase production from palm oil mill effluent
publisher	Science Publications
publishDate	2010
url	http://irep.iium.edu.my/3745/ http://irep.iium.edu.my/3745/ http://irep.iium.edu.my/3745/1/ajessp.2010.66.70.pdf
first_indexed	2023-09-18T20:11:42Z
last_indexed	2023-09-18T20:11:42Z
_version_	1777407520810205184

Statistical optimization of fermentation conditions for cellulase production from palm oil mill effluent

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