Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask

An effective mammalian cell culture based host system is highly desirable for production of bio-therapeutics. There are several strategies to achieve an effective system, one of which is regulation of media coupled with exploitation of inherent characteristics of cell line. In this present study, i...

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Main Authors:	Mohamed, Vasila Packeer, Hashim, Yumi Zuhanis Has-Yun, Mohmad Saberi, Salfarin Ezrina, Ibrahim, Siti Nuramalis, Amid, Azura, Mel, Maizirwan
Format:	Article
Language:	English English
Published:	American-Eurasian Network for Scientific Information 2014
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/38024/ http://irep.iium.edu.my/38024/ http://irep.iium.edu.my/38024/1/Paper_-_Packeer-Mohamed_et_al_2014_%28AEB%29.pdf http://irep.iium.edu.my/38024/4/38024_Effects%20of%20glutamine%20and%20serum_scopus.pdf

id	iium-38024
recordtype	eprints
spelling	iium-380242017-09-19T06:41:47Z http://irep.iium.edu.my/38024/ Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask Mohamed, Vasila Packeer Hashim, Yumi Zuhanis Has-Yun Mohmad Saberi, Salfarin Ezrina Ibrahim, Siti Nuramalis Amid, Azura Mel, Maizirwan Q Science (General) An effective mammalian cell culture based host system is highly desirable for production of bio-therapeutics. There are several strategies to achieve an effective system, one of which is regulation of media coupled with exploitation of inherent characteristics of cell line. In this present study, it is hypothesized that glutamine and serum may affect CHO-K1 cell growth through Insulin-like Growth Factor-I (IGF-I)pathway. The effects of glutamine and serum on IGF-dependent CHO-K1 cell growth in T-flask were studied based on three-level Full Factorial Design generated by SIMCAP+v12 (Umetrics, Sweden). The combination factor of 0.50 mM glutamine and 10 % (v/v) serum was found to be the optimal condition producing 8.87 x 105 cells/ml. The CHO-K1 culture was also found to be able to grow in zero glutamine reaching 16.60 x 105 cells/ ml. Meanwhile, improvement of cell growth can also be achieved in reduced serum level with the presence of IGF-I protein. American-Eurasian Network for Scientific Information 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38024/1/Paper_-_Packeer-Mohamed_et_al_2014_%28AEB%29.pdf application/pdf en http://irep.iium.edu.my/38024/4/38024_Effects%20of%20glutamine%20and%20serum_scopus.pdf Mohamed, Vasila Packeer and Hashim, Yumi Zuhanis Has-Yun and Mohmad Saberi, Salfarin Ezrina and Ibrahim, Siti Nuramalis and Amid, Azura and Mel, Maizirwan (2014) Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask. Advances in Environmental Biology, 8 (3 ( Special Issue )). pp. 831-837. ISSN 1995-0756 E-ISSN 1998-1066 http://www.aensiweb.com/old/aeb/2014/831-837.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Mohamed, Vasila Packeer Hashim, Yumi Zuhanis Has-Yun Mohmad Saberi, Salfarin Ezrina Ibrahim, Siti Nuramalis Amid, Azura Mel, Maizirwan Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask
description	An effective mammalian cell culture based host system is highly desirable for production of bio-therapeutics. There are several strategies to achieve an effective system, one of which is regulation of media coupled with exploitation of inherent characteristics of cell line. In this present study, it is hypothesized that glutamine and serum may affect CHO-K1 cell growth through Insulin-like Growth Factor-I (IGF-I)pathway. The effects of glutamine and serum on IGF-dependent CHO-K1 cell growth in T-flask were studied based on three-level Full Factorial Design generated by SIMCAP+v12 (Umetrics, Sweden). The combination factor of 0.50 mM glutamine and 10 % (v/v) serum was found to be the optimal condition producing 8.87 x 105 cells/ml. The CHO-K1 culture was also found to be able to grow in zero glutamine reaching 16.60 x 105 cells/ ml. Meanwhile, improvement of cell growth can also be achieved in reduced serum level with the presence of IGF-I protein.
format	Article
author	Mohamed, Vasila Packeer Hashim, Yumi Zuhanis Has-Yun Mohmad Saberi, Salfarin Ezrina Ibrahim, Siti Nuramalis Amid, Azura Mel, Maizirwan
author_facet	Mohamed, Vasila Packeer Hashim, Yumi Zuhanis Has-Yun Mohmad Saberi, Salfarin Ezrina Ibrahim, Siti Nuramalis Amid, Azura Mel, Maizirwan
author_sort	Mohamed, Vasila Packeer
title	Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask
title_short	Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask
title_full	Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask
title_fullStr	Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask
title_full_unstemmed	Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask
title_sort	effects of glutamine and serum on igf- dependent cho-k1 cell growth in t-flask
publisher	American-Eurasian Network for Scientific Information
publishDate	2014
url	http://irep.iium.edu.my/38024/ http://irep.iium.edu.my/38024/ http://irep.iium.edu.my/38024/1/Paper_-_Packeer-Mohamed_et_al_2014_%28AEB%29.pdf http://irep.iium.edu.my/38024/4/38024_Effects%20of%20glutamine%20and%20serum_scopus.pdf
first_indexed	2023-09-18T20:54:33Z
last_indexed	2023-09-18T20:54:33Z
_version_	1777410217262186496

Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask

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