Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials

Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials

A pilot-scale production of lipase using palm oil mill effluent (POME) as a fermentation basal medium was carried out, and parameters for immobilization of the produced lipase were optimized. Lipase production in a 300-L bioreactor was performed using two proposed strategies, constant power per volu...

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Main Authors: Asih, Devi Ratna, Alam, Md Zahangir, Salleh, Md. Noor, Salihu, Aliyu
Format: Article
Language:English
English
Published: Japan Oil Chemists' Society 2014
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/38191/
http://irep.iium.edu.my/38191/
http://irep.iium.edu.my/38191/
http://irep.iium.edu.my/38191/1/Paper-JOS-devi.pdf
http://irep.iium.edu.my/38191/4/38191-Pilot-scale%20production%20of%20lipase%20using%20palm%20oil%20mill%20effluent%20as%20a%20basal%20medium_SCOPUS.pdf
id iium-38191
recordtype eprints
spelling iium-381912017-08-10T07:21:51Z http://irep.iium.edu.my/38191/ Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials Asih, Devi Ratna Alam, Md Zahangir Salleh, Md. Noor Salihu, Aliyu TP248.13 Biotechnology A pilot-scale production of lipase using palm oil mill effluent (POME) as a fermentation basal medium was carried out, and parameters for immobilization of the produced lipase were optimized. Lipase production in a 300-L bioreactor was performed using two proposed strategies, constant power per volume (P/V) and constant tip speed. Moreover, lipase immobilization on different materials was also investigated. Lipase production was performed using liquid-state bioconversion of POME as the medium and Candida cylindracea as the inoculum. The fermentation medium was composed of 1% total suspended solids (TSS) of POME, 0.5% (w/v) peptone, 0.7% (v/v) Tween-80, and 2.2% inoculum. The medium composition was decided on the basis of the medium optimization results of a previous study. The fermentation was carried out for 48 h at 30°C and pH 6. The maximum lipase production was 5.72U/mL and 21.34 U/mL, obtained from the scale-up strategies of constant tip speed and P/V, respectively. Four accessible support materials were screened for their potential use in immobilization. The most suitable support material was found to be activated carbon, with a maximum immobilization of 94%. Japan Oil Chemists' Society 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38191/1/Paper-JOS-devi.pdf application/pdf en http://irep.iium.edu.my/38191/4/38191-Pilot-scale%20production%20of%20lipase%20using%20palm%20oil%20mill%20effluent%20as%20a%20basal%20medium_SCOPUS.pdf Asih, Devi Ratna and Alam, Md Zahangir and Salleh, Md. Noor and Salihu, Aliyu (2014) Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials. Journal of Oleo Science, 63 (8). pp. 779-785. ISSN 1345-8957 E-ISSN 1347-3352 http://dx.doi.org/10.5650/jos.ess13187 doi:10.5650/jos.ess13187
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Asih, Devi Ratna
Alam, Md Zahangir
Salleh, Md. Noor
Salihu, Aliyu
Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials
description A pilot-scale production of lipase using palm oil mill effluent (POME) as a fermentation basal medium was carried out, and parameters for immobilization of the produced lipase were optimized. Lipase production in a 300-L bioreactor was performed using two proposed strategies, constant power per volume (P/V) and constant tip speed. Moreover, lipase immobilization on different materials was also investigated. Lipase production was performed using liquid-state bioconversion of POME as the medium and Candida cylindracea as the inoculum. The fermentation medium was composed of 1% total suspended solids (TSS) of POME, 0.5% (w/v) peptone, 0.7% (v/v) Tween-80, and 2.2% inoculum. The medium composition was decided on the basis of the medium optimization results of a previous study. The fermentation was carried out for 48 h at 30°C and pH 6. The maximum lipase production was 5.72U/mL and 21.34 U/mL, obtained from the scale-up strategies of constant tip speed and P/V, respectively. Four accessible support materials were screened for their potential use in immobilization. The most suitable support material was found to be activated carbon, with a maximum immobilization of 94%.
format Article
author Asih, Devi Ratna
Alam, Md Zahangir
Salleh, Md. Noor
Salihu, Aliyu
author_facet Asih, Devi Ratna
Alam, Md Zahangir
Salleh, Md. Noor
Salihu, Aliyu
author_sort Asih, Devi Ratna
title Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials
title_short Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials
title_full Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials
title_fullStr Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials
title_full_unstemmed Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials
title_sort pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials
publisher Japan Oil Chemists' Society
publishDate 2014
url http://irep.iium.edu.my/38191/
http://irep.iium.edu.my/38191/
http://irep.iium.edu.my/38191/
http://irep.iium.edu.my/38191/1/Paper-JOS-devi.pdf
http://irep.iium.edu.my/38191/4/38191-Pilot-scale%20production%20of%20lipase%20using%20palm%20oil%20mill%20effluent%20as%20a%20basal%20medium_SCOPUS.pdf
first_indexed 2023-09-18T20:54:50Z
last_indexed 2023-09-18T20:54:50Z
_version_ 1777410234703151104

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