In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts

The cytocompatibility of novel chitosan derivative sheets and pastes were evaluated in vitro for possible utilization in wound dressing applications for wound healing. In this study, the cytotoxicity of oligochitosan (O-C), N,O-carboxymethyl-chitosan (NO-CMC) and N- carboxymethyl-chitosan (N-CMC)...

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Main Authors:	Abdull Rasad, Mohammad Syaiful Bahari, Halim, Ahmad Sukari, Hashim, Kamaruddin, Abdul Rashid , Ahmad Hazri, Yusof, Norimah, Shamsuddin, Shaharum
Format:	Article
Language:	English
Published:	Elsevier Ltd. 2010
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/38238/ http://irep.iium.edu.my/38238/ http://irep.iium.edu.my/38238/ http://irep.iium.edu.my/38238/1/Carbohydrate_Polymers_Journal.pdf

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recordtype	eprints
spelling	iium-382382014-09-12T02:41:11Z http://irep.iium.edu.my/38238/ In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts Abdull Rasad, Mohammad Syaiful Bahari Halim, Ahmad Sukari Hashim, Kamaruddin Abdul Rashid , Ahmad Hazri Yusof, Norimah Shamsuddin, Shaharum Q Science (General) The cytocompatibility of novel chitosan derivative sheets and pastes were evaluated in vitro for possible utilization in wound dressing applications for wound healing. In this study, the cytotoxicity of oligochitosan (O-C), N,O-carboxymethyl-chitosan (NO-CMC) and N- carboxymethyl-chitosan (N-CMC) derivatives in sheet like and paste forms were evaluated using primary normal human dermal fibroblast cultures and hypertrophic scars; a fibrotic conditions representing a model of altered wound healing with overproduction of extracellular matrix and fibroblast hyperproliferative activity. Cytotoxicities of these chitosan derivatives were assessed using 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl tetrazolium bromide (MTT) assay. The results indicate that both chitosan derivative sheets and pastes have appropriate cytocompatibility and appear promising as safe biomaterials with potential wound healing applications. The SH120 (NO-CMC) derivatives sheet exhibited highest cytocompatibility property and may be regulated by MMP-13 in controlling the cell growth and its expression level. Elsevier Ltd. 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38238/1/Carbohydrate_Polymers_Journal.pdf Abdull Rasad, Mohammad Syaiful Bahari and Halim, Ahmad Sukari and Hashim, Kamaruddin and Abdul Rashid , Ahmad Hazri and Yusof, Norimah and Shamsuddin, Shaharum (2010) In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts. Carbohydrate Polymers, 79. pp. 1094-1100. ISSN 0144-8617 (O) http://www.journals.elsevier.com/carbohydrate-polymers 10.1016/j.carbpol.2009.10.048
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Abdull Rasad, Mohammad Syaiful Bahari Halim, Ahmad Sukari Hashim, Kamaruddin Abdul Rashid , Ahmad Hazri Yusof, Norimah Shamsuddin, Shaharum In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts
description	The cytocompatibility of novel chitosan derivative sheets and pastes were evaluated in vitro for possible utilization in wound dressing applications for wound healing. In this study, the cytotoxicity of oligochitosan (O-C), N,O-carboxymethyl-chitosan (NO-CMC) and N- carboxymethyl-chitosan (N-CMC) derivatives in sheet like and paste forms were evaluated using primary normal human dermal fibroblast cultures and hypertrophic scars; a fibrotic conditions representing a model of altered wound healing with overproduction of extracellular matrix and fibroblast hyperproliferative activity. Cytotoxicities of these chitosan derivatives were assessed using 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl tetrazolium bromide (MTT) assay. The results indicate that both chitosan derivative sheets and pastes have appropriate cytocompatibility and appear promising as safe biomaterials with potential wound healing applications. The SH120 (NO-CMC) derivatives sheet exhibited highest cytocompatibility property and may be regulated by MMP-13 in controlling the cell growth and its expression level.
format	Article
author	Abdull Rasad, Mohammad Syaiful Bahari Halim, Ahmad Sukari Hashim, Kamaruddin Abdul Rashid , Ahmad Hazri Yusof, Norimah Shamsuddin, Shaharum
author_facet	Abdull Rasad, Mohammad Syaiful Bahari Halim, Ahmad Sukari Hashim, Kamaruddin Abdul Rashid , Ahmad Hazri Yusof, Norimah Shamsuddin, Shaharum
author_sort	Abdull Rasad, Mohammad Syaiful Bahari
title	In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts
title_short	In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts
title_full	In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts
title_fullStr	In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts
title_full_unstemmed	In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts
title_sort	in vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts
publisher	Elsevier Ltd.
publishDate	2010
url	http://irep.iium.edu.my/38238/ http://irep.iium.edu.my/38238/ http://irep.iium.edu.my/38238/ http://irep.iium.edu.my/38238/1/Carbohydrate_Polymers_Journal.pdf
first_indexed	2023-09-18T20:54:55Z
last_indexed	2023-09-18T20:54:55Z
_version_	1777410239962808320

In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts

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