In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts

The cytocompatibility of novel chitosan derivative sheets and pastes were evaluated in vitro for possible utilization in wound dressing applications for wound healing. In this study, the cytotoxicity of oligochitosan (O-C), N,O-carboxymethyl-chitosan (NO-CMC) and N- carboxymethyl-chitosan (N-CMC)...

Full description

Bibliographic Details
Main Authors: Abdull Rasad, Mohammad Syaiful Bahari, Halim, Ahmad Sukari, Hashim, Kamaruddin, Abdul Rashid , Ahmad Hazri, Yusof, Norimah, Shamsuddin, Shaharum
Format: Article
Language:English
Published: Elsevier Ltd. 2010
Subjects:
Online Access:http://irep.iium.edu.my/38238/
http://irep.iium.edu.my/38238/
http://irep.iium.edu.my/38238/
http://irep.iium.edu.my/38238/1/Carbohydrate_Polymers_Journal.pdf
id iium-38238
recordtype eprints
spelling iium-382382014-09-12T02:41:11Z http://irep.iium.edu.my/38238/ In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts Abdull Rasad, Mohammad Syaiful Bahari Halim, Ahmad Sukari Hashim, Kamaruddin Abdul Rashid , Ahmad Hazri Yusof, Norimah Shamsuddin, Shaharum Q Science (General) The cytocompatibility of novel chitosan derivative sheets and pastes were evaluated in vitro for possible utilization in wound dressing applications for wound healing. In this study, the cytotoxicity of oligochitosan (O-C), N,O-carboxymethyl-chitosan (NO-CMC) and N- carboxymethyl-chitosan (N-CMC) derivatives in sheet like and paste forms were evaluated using primary normal human dermal fibroblast cultures and hypertrophic scars; a fibrotic conditions representing a model of altered wound healing with overproduction of extracellular matrix and fibroblast hyperproliferative activity. Cytotoxicities of these chitosan derivatives were assessed using 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl tetrazolium bromide (MTT) assay. The results indicate that both chitosan derivative sheets and pastes have appropriate cytocompatibility and appear promising as safe biomaterials with potential wound healing applications. The SH120 (NO-CMC) derivatives sheet exhibited highest cytocompatibility property and may be regulated by MMP-13 in controlling the cell growth and its expression level. Elsevier Ltd. 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38238/1/Carbohydrate_Polymers_Journal.pdf Abdull Rasad, Mohammad Syaiful Bahari and Halim, Ahmad Sukari and Hashim, Kamaruddin and Abdul Rashid , Ahmad Hazri and Yusof, Norimah and Shamsuddin, Shaharum (2010) In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts. Carbohydrate Polymers, 79. pp. 1094-1100. ISSN 0144-8617 (O) http://www.journals.elsevier.com/carbohydrate-polymers 10.1016/j.carbpol.2009.10.048
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic Q Science (General)
spellingShingle Q Science (General)
Abdull Rasad, Mohammad Syaiful Bahari
Halim, Ahmad Sukari
Hashim, Kamaruddin
Abdul Rashid , Ahmad Hazri
Yusof, Norimah
Shamsuddin, Shaharum
In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts
description The cytocompatibility of novel chitosan derivative sheets and pastes were evaluated in vitro for possible utilization in wound dressing applications for wound healing. In this study, the cytotoxicity of oligochitosan (O-C), N,O-carboxymethyl-chitosan (NO-CMC) and N- carboxymethyl-chitosan (N-CMC) derivatives in sheet like and paste forms were evaluated using primary normal human dermal fibroblast cultures and hypertrophic scars; a fibrotic conditions representing a model of altered wound healing with overproduction of extracellular matrix and fibroblast hyperproliferative activity. Cytotoxicities of these chitosan derivatives were assessed using 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl tetrazolium bromide (MTT) assay. The results indicate that both chitosan derivative sheets and pastes have appropriate cytocompatibility and appear promising as safe biomaterials with potential wound healing applications. The SH120 (NO-CMC) derivatives sheet exhibited highest cytocompatibility property and may be regulated by MMP-13 in controlling the cell growth and its expression level.
format Article
author Abdull Rasad, Mohammad Syaiful Bahari
Halim, Ahmad Sukari
Hashim, Kamaruddin
Abdul Rashid , Ahmad Hazri
Yusof, Norimah
Shamsuddin, Shaharum
author_facet Abdull Rasad, Mohammad Syaiful Bahari
Halim, Ahmad Sukari
Hashim, Kamaruddin
Abdul Rashid , Ahmad Hazri
Yusof, Norimah
Shamsuddin, Shaharum
author_sort Abdull Rasad, Mohammad Syaiful Bahari
title In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts
title_short In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts
title_full In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts
title_fullStr In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts
title_full_unstemmed In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts
title_sort in vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts
publisher Elsevier Ltd.
publishDate 2010
url http://irep.iium.edu.my/38238/
http://irep.iium.edu.my/38238/
http://irep.iium.edu.my/38238/
http://irep.iium.edu.my/38238/1/Carbohydrate_Polymers_Journal.pdf
first_indexed 2023-09-18T20:54:55Z
last_indexed 2023-09-18T20:54:55Z
_version_ 1777410239962808320