Statistical screening and optimization of factors that influence lipase production from palm kernel cake using candida cylindracea in solid-state bioconversion

Although lipases are important class of industrial enzymes but the high cost of their production limits their applications. In this study, an abundant raw material in Malaysia, palm kernel cake (PKC), was investigated as a substrate ina fermentation process to produce lipase using solid-state biocon...

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Main Authors:	Elgharbawy, Amal Ahmed, Alam, Md Zahangir, Mohd. Salleh, Hamzah
Format:	Article
Language:	English
Published:	Journal of Pure and Applied Microbiology 2014
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/38272/ http://irep.iium.edu.my/38272/ http://irep.iium.edu.my/38272/1/Paper-JPAM-amal.pdf

id	iium-38272
recordtype	eprints
spelling	iium-382722017-08-03T06:34:28Z http://irep.iium.edu.my/38272/ Statistical screening and optimization of factors that influence lipase production from palm kernel cake using candida cylindracea in solid-state bioconversion Elgharbawy, Amal Ahmed Alam, Md Zahangir Mohd. Salleh, Hamzah TP248.13 Biotechnology Although lipases are important class of industrial enzymes but the high cost of their production limits their applications. In this study, an abundant raw material in Malaysia, palm kernel cake (PKC), was investigated as a substrate ina fermentation process to produce lipase using solid-state bioconversion (SSB) with minimum requirements of machinery and external nutrients. This requires an optimization process in order to achieve maximum production with the minimum requirements. Optimization for the parameters that influence lipase production from Candida cylindracea was conducted using statistical experimental designs including Plackett-Burman (PB) design, one-factor- at-a-time (OFAT) and response surface methodology (RSM). Optimization enhanced lipase production over 4-fold compared to the screening stage, in PKC media supplemented with 1.5% (w/w) yeast extract, 2.0% (v/w) Tween-80, 0.5% (v/w) olive oil and 7.0% (v/w) inoculum at pH 7.0 and 30oC within 72 hrs fermentation. Maximum activity of 400?2 U/g dry PKC was achieved. The analysis of variance (ANOVA) indicated that the model was significant (p<0.05) with coefficient of determination (R2) 0.9893 which was very close to the adjusted R2 (0.9816) pointing the reliability of the model. Journal of Pure and Applied Microbiology 2014-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38272/1/Paper-JPAM-amal.pdf Elgharbawy, Amal Ahmed and Alam, Md Zahangir and Mohd. Salleh, Hamzah (2014) Statistical screening and optimization of factors that influence lipase production from palm kernel cake using candida cylindracea in solid-state bioconversion. Journal of Pure and Applied Microbiology, 8 (s.ed 1). pp. 691-705. ISSN 0973-7510 http://www.microbiologyjournal.org/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Elgharbawy, Amal Ahmed Alam, Md Zahangir Mohd. Salleh, Hamzah Statistical screening and optimization of factors that influence lipase production from palm kernel cake using candida cylindracea in solid-state bioconversion
description	Although lipases are important class of industrial enzymes but the high cost of their production limits their applications. In this study, an abundant raw material in Malaysia, palm kernel cake (PKC), was investigated as a substrate ina fermentation process to produce lipase using solid-state bioconversion (SSB) with minimum requirements of machinery and external nutrients. This requires an optimization process in order to achieve maximum production with the minimum requirements. Optimization for the parameters that influence lipase production from Candida cylindracea was conducted using statistical experimental designs including Plackett-Burman (PB) design, one-factor- at-a-time (OFAT) and response surface methodology (RSM). Optimization enhanced lipase production over 4-fold compared to the screening stage, in PKC media supplemented with 1.5% (w/w) yeast extract, 2.0% (v/w) Tween-80, 0.5% (v/w) olive oil and 7.0% (v/w) inoculum at pH 7.0 and 30oC within 72 hrs fermentation. Maximum activity of 400?2 U/g dry PKC was achieved. The analysis of variance (ANOVA) indicated that the model was significant (p<0.05) with coefficient of determination (R2) 0.9893 which was very close to the adjusted R2 (0.9816) pointing the reliability of the model.
format	Article
author	Elgharbawy, Amal Ahmed Alam, Md Zahangir Mohd. Salleh, Hamzah
author_facet	Elgharbawy, Amal Ahmed Alam, Md Zahangir Mohd. Salleh, Hamzah
author_sort	Elgharbawy, Amal Ahmed
title	Statistical screening and optimization of factors that influence lipase production from palm kernel cake using candida cylindracea in solid-state bioconversion
title_short	Statistical screening and optimization of factors that influence lipase production from palm kernel cake using candida cylindracea in solid-state bioconversion
title_full	Statistical screening and optimization of factors that influence lipase production from palm kernel cake using candida cylindracea in solid-state bioconversion
title_fullStr	Statistical screening and optimization of factors that influence lipase production from palm kernel cake using candida cylindracea in solid-state bioconversion
title_full_unstemmed	Statistical screening and optimization of factors that influence lipase production from palm kernel cake using candida cylindracea in solid-state bioconversion
title_sort	statistical screening and optimization of factors that influence lipase production from palm kernel cake using candida cylindracea in solid-state bioconversion
publisher	Journal of Pure and Applied Microbiology
publishDate	2014
url	http://irep.iium.edu.my/38272/ http://irep.iium.edu.my/38272/ http://irep.iium.edu.my/38272/1/Paper-JPAM-amal.pdf
first_indexed	2023-09-18T20:54:58Z
last_indexed	2023-09-18T20:54:58Z
_version_	1777410243434643456

Statistical screening and optimization of factors that influence lipase production from palm kernel cake using candida cylindracea in solid-state bioconversion

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