Immobilization of lipase enzyme by low cost material: a statistical approach

Immobilization of lipase enzyme produced from palm oil mill effluent (POME) by the activated carbon (A C) among the low cost support materials was optimized. The results indicated that immobilization of 94% was achieved by AC as the most suitable support material. A sequential optimization strateg...

Full description

Bibliographic Details
Main Authors:	Alam, Md Zahangir, Asih, Devi Ratna, Salleh, Md. Noor
Format:	Article
Language:	English
Published:	World Academy of Science, Engineering and Technology 2014
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/38274/ http://irep.iium.edu.my/38274/1/Paper-Immo-Devi.pdf

id	iium-38274
recordtype	eprints
spelling	iium-382742018-06-12T07:44:25Z http://irep.iium.edu.my/38274/ Immobilization of lipase enzyme by low cost material: a statistical approach Alam, Md Zahangir Asih, Devi Ratna Salleh, Md. Noor TP248.13 Biotechnology Immobilization of lipase enzyme produced from palm oil mill effluent (POME) by the activated carbon (A C) among the low cost support materials was optimized. The results indicated that immobilization of 94% was achieved by AC as the most suitable support material. A sequential optimization strategy based on a statistical experimental design, including one-fact or-at-a-time (OFAT) method was used to determine the equilibrium time. Three components influencing lipase immobilization were optimized by theresponse surface methodology (RSM) based on the face-centered central composite design (FCCCD). On the statistical analysis of the results, the optimum enzyme concentration loading, agitation rate and carbon active dosage were found to be 30 U/ml, 300 rpm and 8 g/L respectively, with a maximum immobilization activity of 3732.9 U/g-AC after 2 hrs of immobilization. Analysis of variance (ANOVA) showed a high regression coefficient (R2) of 0.999, which indicated a satisfactory fit of the model with the experimental data. The parameters were statistically significant at p<0.05. World Academy of Science, Engineering and Technology 2014-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38274/1/Paper-Immo-Devi.pdf Alam, Md Zahangir and Asih, Devi Ratna and Salleh, Md. Noor (2014) Immobilization of lipase enzyme by low cost material: a statistical approach. International Journal of Biological, Veterinary, Agricultural and Food Engineering, 8 (6). pp. 522-525.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Alam, Md Zahangir Asih, Devi Ratna Salleh, Md. Noor Immobilization of lipase enzyme by low cost material: a statistical approach
description	Immobilization of lipase enzyme produced from palm oil mill effluent (POME) by the activated carbon (A C) among the low cost support materials was optimized. The results indicated that immobilization of 94% was achieved by AC as the most suitable support material. A sequential optimization strategy based on a statistical experimental design, including one-fact or-at-a-time (OFAT) method was used to determine the equilibrium time. Three components influencing lipase immobilization were optimized by theresponse surface methodology (RSM) based on the face-centered central composite design (FCCCD). On the statistical analysis of the results, the optimum enzyme concentration loading, agitation rate and carbon active dosage were found to be 30 U/ml, 300 rpm and 8 g/L respectively, with a maximum immobilization activity of 3732.9 U/g-AC after 2 hrs of immobilization. Analysis of variance (ANOVA) showed a high regression coefficient (R2) of 0.999, which indicated a satisfactory fit of the model with the experimental data. The parameters were statistically significant at p<0.05.
format	Article
author	Alam, Md Zahangir Asih, Devi Ratna Salleh, Md. Noor
author_facet	Alam, Md Zahangir Asih, Devi Ratna Salleh, Md. Noor
author_sort	Alam, Md Zahangir
title	Immobilization of lipase enzyme by low cost material: a statistical approach
title_short	Immobilization of lipase enzyme by low cost material: a statistical approach
title_full	Immobilization of lipase enzyme by low cost material: a statistical approach
title_fullStr	Immobilization of lipase enzyme by low cost material: a statistical approach
title_full_unstemmed	Immobilization of lipase enzyme by low cost material: a statistical approach
title_sort	immobilization of lipase enzyme by low cost material: a statistical approach
publisher	World Academy of Science, Engineering and Technology
publishDate	2014
url	http://irep.iium.edu.my/38274/ http://irep.iium.edu.my/38274/1/Paper-Immo-Devi.pdf
first_indexed	2023-09-18T20:54:59Z
last_indexed	2023-09-18T20:54:59Z
_version_	1777410243706224640

Immobilization of lipase enzyme by low cost material: a statistical approach

Similar Items