Dose related proliferative changes in the prostate of rats induced by eurycoma longifolia Jack (Tongkat Ali) extract

Eurycoma longifolia jack(E.L) root extract has become famous for giving enhanced sexual performance ability through elevation of serum testosterone levels in males. However, although very widely used, it’s possible adverse effects are poorly studied .The objective of this study is to find out if the...

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Main Authors:	Faisal, Ghasak Ghazi, Ezzat Mustafa, Basma, Mustafa, Nazih Shaban, Najmuldeen, Ghazi Faisal
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	R Medicine (General) RB Pathology
Online Access:	http://irep.iium.edu.my/38406/ http://irep.iium.edu.my/38406/4/dose_related_proliferative_IRIE14.pdf

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recordtype	eprints
spelling	iium-384062015-01-06T11:03:59Z http://irep.iium.edu.my/38406/ Dose related proliferative changes in the prostate of rats induced by eurycoma longifolia Jack (Tongkat Ali) extract Faisal, Ghasak Ghazi Ezzat Mustafa, Basma Mustafa, Nazih Shaban Najmuldeen, Ghazi Faisal R Medicine (General) RB Pathology Eurycoma longifolia jack(E.L) root extract has become famous for giving enhanced sexual performance ability through elevation of serum testosterone levels in males. However, although very widely used, it’s possible adverse effects are poorly studied .The objective of this study is to find out if there are any pathological changes that occur in the prostate of rats due to consumption of E.L. root extract. Methodology:45 male rats where used in this study , they were divided into three groups ,group(A) contain 5 rats that represent the control group who did not receive any treatment, group (B) with 20 rats ,received 5mg/Kg twice daily of 100:1 freeze dried water extract of Eurycoma longifolia Jack roots and group (C) with 20 rats received 10mg/kg body weight twice daily of the same extract .The duration of treatment for both groups was 4 weeks. At the end of the experiment, blood was obtained from the rats and serum testosterone level (T) was analyzed. The rats were then terminated and the prostate was removed, weighed and its dimensions measured, then it was processed into haematoxiline and eosin stained slides which were observed under light microscope. Results: Group C showed a significant increase in prostate size, weight and serum T. Histopathological examination of the specimens revealed that there were no significant observable changes in group B in comparison with the control group while group C showed a marked cell proliferation of the prostatic gland lining epithelium that appeared as stratification and intraglandular villus formation of the usual single layer columnar epithelium. Areas of mild dysplastic changes were observed in the lining epithelium characterized by nuclear enlargement and irregularities in the nuclear membrane and hyperchromatism. These dysplastic changes were equivalent to low grade prostatic intraepithelial neoplasia PIN. Conclusion: E.L. root extract can cause abnormal proliferation in the prostatic epithelium and these changes are dose dependant. 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/38406/4/dose_related_proliferative_IRIE14.pdf Faisal, Ghasak Ghazi and Ezzat Mustafa, Basma and Mustafa, Nazih Shaban and Najmuldeen, Ghazi Faisal (2014) Dose related proliferative changes in the prostate of rats induced by eurycoma longifolia Jack (Tongkat Ali) extract. In: International Research Invention and Innovation Exhibition (IRIIE) 2014, 11-12 June 2014, Gombak, Kuala Lumpur.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General) RB Pathology
spellingShingle	R Medicine (General) RB Pathology Faisal, Ghasak Ghazi Ezzat Mustafa, Basma Mustafa, Nazih Shaban Najmuldeen, Ghazi Faisal Dose related proliferative changes in the prostate of rats induced by eurycoma longifolia Jack (Tongkat Ali) extract
description	Eurycoma longifolia jack(E.L) root extract has become famous for giving enhanced sexual performance ability through elevation of serum testosterone levels in males. However, although very widely used, it’s possible adverse effects are poorly studied .The objective of this study is to find out if there are any pathological changes that occur in the prostate of rats due to consumption of E.L. root extract. Methodology:45 male rats where used in this study , they were divided into three groups ,group(A) contain 5 rats that represent the control group who did not receive any treatment, group (B) with 20 rats ,received 5mg/Kg twice daily of 100:1 freeze dried water extract of Eurycoma longifolia Jack roots and group (C) with 20 rats received 10mg/kg body weight twice daily of the same extract .The duration of treatment for both groups was 4 weeks. At the end of the experiment, blood was obtained from the rats and serum testosterone level (T) was analyzed. The rats were then terminated and the prostate was removed, weighed and its dimensions measured, then it was processed into haematoxiline and eosin stained slides which were observed under light microscope. Results: Group C showed a significant increase in prostate size, weight and serum T. Histopathological examination of the specimens revealed that there were no significant observable changes in group B in comparison with the control group while group C showed a marked cell proliferation of the prostatic gland lining epithelium that appeared as stratification and intraglandular villus formation of the usual single layer columnar epithelium. Areas of mild dysplastic changes were observed in the lining epithelium characterized by nuclear enlargement and irregularities in the nuclear membrane and hyperchromatism. These dysplastic changes were equivalent to low grade prostatic intraepithelial neoplasia PIN. Conclusion: E.L. root extract can cause abnormal proliferation in the prostatic epithelium and these changes are dose dependant.
format	Conference or Workshop Item
author	Faisal, Ghasak Ghazi Ezzat Mustafa, Basma Mustafa, Nazih Shaban Najmuldeen, Ghazi Faisal
author_facet	Faisal, Ghasak Ghazi Ezzat Mustafa, Basma Mustafa, Nazih Shaban Najmuldeen, Ghazi Faisal
author_sort	Faisal, Ghasak Ghazi
title	Dose related proliferative changes in the prostate of rats induced by eurycoma longifolia Jack (Tongkat Ali) extract
title_short	Dose related proliferative changes in the prostate of rats induced by eurycoma longifolia Jack (Tongkat Ali) extract
title_full	Dose related proliferative changes in the prostate of rats induced by eurycoma longifolia Jack (Tongkat Ali) extract
title_fullStr	Dose related proliferative changes in the prostate of rats induced by eurycoma longifolia Jack (Tongkat Ali) extract
title_full_unstemmed	Dose related proliferative changes in the prostate of rats induced by eurycoma longifolia Jack (Tongkat Ali) extract
title_sort	dose related proliferative changes in the prostate of rats induced by eurycoma longifolia jack (tongkat ali) extract
publishDate	2014
url	http://irep.iium.edu.my/38406/ http://irep.iium.edu.my/38406/4/dose_related_proliferative_IRIE14.pdf
first_indexed	2023-09-18T20:55:11Z
last_indexed	2023-09-18T20:55:11Z
_version_	1777410256431742976

Dose related proliferative changes in the prostate of rats induced by eurycoma longifolia Jack (Tongkat Ali) extract

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