Optimization on cell disruption of E. coli BL21-AI expressing recombinant bromelain
Recent advancements in recombinant DNA technology proved to be a promising and effective approach for more sustainable large scale productions of many therapeutic proteins. Nevertheless, since this approach involves expression of proteins in a nonnative host microorganism, the overall production...
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Journal of Pure and Applied Microbiology
2014
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iium-384512018-06-12T02:36:03Z http://irep.iium.edu.my/38451/ Optimization on cell disruption of E. coli BL21-AI expressing recombinant bromelain Othman, Muhd. Ezza Faiez Amid, Azura Jimat, Dzun Noraini Jamaluddin, Mohd Jamil Aizat TP248.13 Biotechnology Recent advancements in recombinant DNA technology proved to be a promising and effective approach for more sustainable large scale productions of many therapeutic proteins. Nevertheless, since this approach involves expression of proteins in a nonnative host microorganism, the overall production processes are not straight-forward due to several common challenges, such as protein degradation, especially during cell disruption stage. As the process has been subjected to both protein and host-specific, a systematic process conditioning for maximal production of recombinant protein is therefore required. In this study, a simple approach to determine optimal conditions for cell disruption using ultrasonication to isolate recombinant bromelain from E. coli BL21- AI is reported. Suspension cells were lysed using ultrasonication which transmit sound wave in other to break the cell wall. An optimized condition was obtained by response surface methodology (RSM). A three factor face-centered central composite design (FCCD) was applied to obtain the optimal process conditions consisting of amplitude, cycle and bursting period. The prediction model was further validated. Therefore, under the optimal conditions, having 20% amplitude, 0.5s cycle, and 1 minute bursting period in three times process, the specific enzyme activity of the recombinant Journal of Pure and Applied Microbiology 2014-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38451/27/May_Ezza_JPAM.pdf application/pdf en http://irep.iium.edu.my/38451/29/38451_Optimization%20on%20cell%20disruption.SCOPUS.pdf Othman, Muhd. Ezza Faiez and Amid, Azura and Jimat, Dzun Noraini and Jamaluddin, Mohd Jamil Aizat (2014) Optimization on cell disruption of E. coli BL21-AI expressing recombinant bromelain. Journal of Pure and Applied Microbiology, 8 (s.ed 1). pp. 797-802. ISSN 0973-7510 http://www.microbiologyjournal.org/ |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English English |
| topic |
TP248.13 Biotechnology |
| spellingShingle |
TP248.13 Biotechnology Othman, Muhd. Ezza Faiez Amid, Azura Jimat, Dzun Noraini Jamaluddin, Mohd Jamil Aizat Optimization on cell disruption of E. coli BL21-AI expressing recombinant bromelain |
| description |
Recent advancements in recombinant DNA technology proved to be a promising
and effective approach for more sustainable large scale productions of many therapeutic
proteins. Nevertheless, since this approach involves expression of proteins in a nonnative
host microorganism, the overall production processes are not straight-forward
due to several common challenges, such as protein degradation, especially during cell
disruption stage. As the process has been subjected to both protein and host-specific, a
systematic process conditioning for maximal production of recombinant protein is
therefore required. In this study, a simple approach to determine optimal conditions for
cell disruption using ultrasonication to isolate recombinant bromelain from E. coli BL21-
AI is reported. Suspension cells were lysed using ultrasonication which transmit sound
wave in other to break the cell wall. An optimized condition was obtained by response
surface methodology (RSM). A three factor face-centered central composite design (FCCD)
was applied to obtain the optimal process conditions consisting of amplitude, cycle and
bursting period. The prediction model was further validated. Therefore, under the optimal
conditions, having 20% amplitude, 0.5s cycle, and 1 minute bursting period in three
times process, the specific enzyme activity of the recombinant |
| format |
Article |
| author |
Othman, Muhd. Ezza Faiez Amid, Azura Jimat, Dzun Noraini Jamaluddin, Mohd Jamil Aizat |
| author_facet |
Othman, Muhd. Ezza Faiez Amid, Azura Jimat, Dzun Noraini Jamaluddin, Mohd Jamil Aizat |
| author_sort |
Othman, Muhd. Ezza Faiez |
| title |
Optimization on cell disruption of E. coli BL21-AI
expressing recombinant bromelain |
| title_short |
Optimization on cell disruption of E. coli BL21-AI
expressing recombinant bromelain |
| title_full |
Optimization on cell disruption of E. coli BL21-AI
expressing recombinant bromelain |
| title_fullStr |
Optimization on cell disruption of E. coli BL21-AI
expressing recombinant bromelain |
| title_full_unstemmed |
Optimization on cell disruption of E. coli BL21-AI
expressing recombinant bromelain |
| title_sort |
optimization on cell disruption of e. coli bl21-ai
expressing recombinant bromelain |
| publisher |
Journal of Pure and Applied Microbiology |
| publishDate |
2014 |
| url |
http://irep.iium.edu.my/38451/ http://irep.iium.edu.my/38451/ http://irep.iium.edu.my/38451/27/May_Ezza_JPAM.pdf http://irep.iium.edu.my/38451/29/38451_Optimization%20on%20cell%20disruption.SCOPUS.pdf |
| first_indexed |
2023-09-18T20:55:15Z |
| last_indexed |
2023-09-18T20:55:15Z |
| _version_ |
1777410260934328320 |