Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction

Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction

Horse mango (Mangifera foetida Lour) fruit is known to produce latex with a blistering effect upon contact with human skin. In this study, it was chosen as a source of protease, and the effect of the extraction process on its protease activity was evaluated. The crude enzyme was extracted from the k...

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Main Authors: Ahmad, Mohammad Norazmi, Ibrahim, Izyanti, Yarmo, Mohd Ambar, Said, Mamot
Format: Article
Language:English
Published: Malaysia Institute of Chemistry 2013
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/38466/
http://irep.iium.edu.my/38466/
http://irep.iium.edu.my/38466/1/Malaysian_Journal_of_Chemistry%2C_2013%2C_Vol._15%2C_No._1%2C_020_%E2%80%93_026.pdf
id iium-38466
recordtype eprints
spelling iium-384662016-10-20T04:21:18Z http://irep.iium.edu.my/38466/ Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction Ahmad, Mohammad Norazmi Ibrahim, Izyanti Yarmo, Mohd Ambar Said, Mamot QD Chemistry Horse mango (Mangifera foetida Lour) fruit is known to produce latex with a blistering effect upon contact with human skin. In this study, it was chosen as a source of protease, and the effect of the extraction process on its protease activity was evaluated. The crude enzyme was extracted from the kernels and extraction was optimized by a response surface methodology (RSM) using a central composite rotatable design (CCRD). Based on the RSM model generated, optimal extraction conditions were obtained at pH 6.0, 8.16 mM CaCl2, 5.0% Triton X-100, and 10.0 mM DTT. Polyvinylpolypyrrolidone (PVPP) and Dowex 2-X8 were added to the medium extraction to remove the polyphenols from the crude extract. The result indicated that, the media which was supplemented with the polyphenols binders acquired the high purified protease compared to the control (media without PVPP and Dowex 2-X8). Malaysia Institute of Chemistry 2013-03-20 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38466/1/Malaysian_Journal_of_Chemistry%2C_2013%2C_Vol._15%2C_No._1%2C_020_%E2%80%93_026.pdf Ahmad, Mohammad Norazmi and Ibrahim, Izyanti and Yarmo, Mohd Ambar and Said, Mamot (2013) Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction. Protease of the Horse Mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction, 15 (1). pp. 20-26. ISSN 1511-2292 http://www.ikm.org.my/index.php?option=com_content&view=article&id=225&Itemid=389
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
spellingShingle QD Chemistry
Ahmad, Mohammad Norazmi
Ibrahim, Izyanti
Yarmo, Mohd Ambar
Said, Mamot
Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction
description Horse mango (Mangifera foetida Lour) fruit is known to produce latex with a blistering effect upon contact with human skin. In this study, it was chosen as a source of protease, and the effect of the extraction process on its protease activity was evaluated. The crude enzyme was extracted from the kernels and extraction was optimized by a response surface methodology (RSM) using a central composite rotatable design (CCRD). Based on the RSM model generated, optimal extraction conditions were obtained at pH 6.0, 8.16 mM CaCl2, 5.0% Triton X-100, and 10.0 mM DTT. Polyvinylpolypyrrolidone (PVPP) and Dowex 2-X8 were added to the medium extraction to remove the polyphenols from the crude extract. The result indicated that, the media which was supplemented with the polyphenols binders acquired the high purified protease compared to the control (media without PVPP and Dowex 2-X8).
format Article
author Ahmad, Mohammad Norazmi
Ibrahim, Izyanti
Yarmo, Mohd Ambar
Said, Mamot
author_facet Ahmad, Mohammad Norazmi
Ibrahim, Izyanti
Yarmo, Mohd Ambar
Said, Mamot
author_sort Ahmad, Mohammad Norazmi
title Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction
title_short Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction
title_full Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction
title_fullStr Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction
title_full_unstemmed Protease of the horse mango (Mangifera foetida Lour) Kernel: Optimum Conditions for Extraction
title_sort protease of the horse mango (mangifera foetida lour) kernel: optimum conditions for extraction
publisher Malaysia Institute of Chemistry
publishDate 2013
url http://irep.iium.edu.my/38466/
http://irep.iium.edu.my/38466/
http://irep.iium.edu.my/38466/1/Malaysian_Journal_of_Chemistry%2C_2013%2C_Vol._15%2C_No._1%2C_020_%E2%80%93_026.pdf
first_indexed 2023-09-18T20:55:17Z
last_indexed 2023-09-18T20:55:17Z
_version_ 1777410262521872384

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