Muscle Creatine Kinase (M-CK), Aldolase A and Voltage Dependent Anion Channel 2 (VDAC2) as Potential Biomarkers for Identification of Electrically Stimulated Chicken Muscle
Water bath stunning in commercial slaughter houses is a common practice and economical. However, the conditions applied for the stunning process may vary from one slaughterhouse to another and has opened up doors for food adulteration to take place. In this study, proteomics approaches have been...
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Journal of Pure and Applied Microbiology
2014
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| Online Access: | http://irep.iium.edu.my/38508/ http://irep.iium.edu.my/38508/ http://irep.iium.edu.my/38508/1/May_Shahida_JPAM.pdf http://irep.iium.edu.my/38508/3/38508_Muscle%20Creatine%20Kinase%20%28M-CK%29_SCOPUS.pdf |
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iium-385082017-09-25T13:07:23Z http://irep.iium.edu.my/38508/ Muscle Creatine Kinase (M-CK), Aldolase A and Voltage Dependent Anion Channel 2 (VDAC2) as Potential Biomarkers for Identification of Electrically Stimulated Chicken Muscle Amid, Azura Abu Samah, Norshahida Yusof, Faridah TP248.13 Biotechnology Water bath stunning in commercial slaughter houses is a common practice and economical. However, the conditions applied for the stunning process may vary from one slaughterhouse to another and has opened up doors for food adulteration to take place. In this study, proteomics approaches have been used to study the effect of different current and voltages during stunning on the protein expression of the chickens. Protein profiles of the chickens muscle were constructed in order to detect any dufferences in protein expression and modifications. The protein muscle profiles of stunning conditions were compared to the non-stunned. After analysis using 2D Platinum ImageMaster 6.0 software and mass spectrometry identification, Muscle creatine kinase (M-CK), AldolaseA and Voltage Dependent Anion Channel 2 (VDAC2) were identified to be over expressed in the muscle sample of stunned chicken. The over expression of candidate proteins was confirmed at the transcriptional level of RNA expression by Real Time PCR. In conclusion, all three candidate proteins may serve as potential biomarkers for electrically stimulated chicken’s muscle. The existence of these biomarkers will help to monitor the slaughtering and stunning process in future. Journal of Pure and Applied Microbiology 2014-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38508/1/May_Shahida_JPAM.pdf application/pdf en http://irep.iium.edu.my/38508/3/38508_Muscle%20Creatine%20Kinase%20%28M-CK%29_SCOPUS.pdf Amid, Azura and Abu Samah, Norshahida and Yusof, Faridah (2014) Muscle Creatine Kinase (M-CK), Aldolase A and Voltage Dependent Anion Channel 2 (VDAC2) as Potential Biomarkers for Identification of Electrically Stimulated Chicken Muscle. Journal of Pure and Applied Microbiology, 8 (s.ed 1). pp. 707-718. ISSN 0973-7510 http://www.microbiologyjournal.org/ |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English English |
| topic |
TP248.13 Biotechnology |
| spellingShingle |
TP248.13 Biotechnology Amid, Azura Abu Samah, Norshahida Yusof, Faridah Muscle Creatine Kinase (M-CK), Aldolase A and Voltage Dependent Anion Channel 2 (VDAC2) as Potential Biomarkers for Identification of Electrically Stimulated Chicken Muscle |
| description |
Water bath stunning in commercial slaughter houses is a common practice and
economical. However, the conditions applied for the stunning process may vary from one
slaughterhouse to another and has opened up doors for food adulteration to take place.
In this study, proteomics approaches have been used to study the effect of different
current and voltages during stunning on the protein expression of the chickens. Protein
profiles of the chickens muscle were constructed in order to detect any dufferences in
protein expression and modifications. The protein muscle profiles of stunning conditions
were compared to the non-stunned. After analysis using 2D Platinum ImageMaster 6.0
software and mass spectrometry identification, Muscle creatine kinase (M-CK), AldolaseA
and Voltage Dependent Anion Channel 2 (VDAC2) were identified to be over expressed in
the muscle sample of stunned chicken. The over expression of candidate proteins was
confirmed at the transcriptional level of RNA expression by Real Time PCR. In conclusion,
all three candidate proteins may serve as potential biomarkers for electrically stimulated
chicken’s muscle. The existence of these biomarkers will help to monitor the slaughtering
and stunning process in future. |
| format |
Article |
| author |
Amid, Azura Abu Samah, Norshahida Yusof, Faridah |
| author_facet |
Amid, Azura Abu Samah, Norshahida Yusof, Faridah |
| author_sort |
Amid, Azura |
| title |
Muscle Creatine Kinase (M-CK), Aldolase A and Voltage
Dependent Anion Channel 2 (VDAC2) as Potential Biomarkers
for Identification of Electrically Stimulated Chicken Muscle |
| title_short |
Muscle Creatine Kinase (M-CK), Aldolase A and Voltage
Dependent Anion Channel 2 (VDAC2) as Potential Biomarkers
for Identification of Electrically Stimulated Chicken Muscle |
| title_full |
Muscle Creatine Kinase (M-CK), Aldolase A and Voltage
Dependent Anion Channel 2 (VDAC2) as Potential Biomarkers
for Identification of Electrically Stimulated Chicken Muscle |
| title_fullStr |
Muscle Creatine Kinase (M-CK), Aldolase A and Voltage
Dependent Anion Channel 2 (VDAC2) as Potential Biomarkers
for Identification of Electrically Stimulated Chicken Muscle |
| title_full_unstemmed |
Muscle Creatine Kinase (M-CK), Aldolase A and Voltage
Dependent Anion Channel 2 (VDAC2) as Potential Biomarkers
for Identification of Electrically Stimulated Chicken Muscle |
| title_sort |
muscle creatine kinase (m-ck), aldolase a and voltage
dependent anion channel 2 (vdac2) as potential biomarkers
for identification of electrically stimulated chicken muscle |
| publisher |
Journal of Pure and Applied Microbiology |
| publishDate |
2014 |
| url |
http://irep.iium.edu.my/38508/ http://irep.iium.edu.my/38508/ http://irep.iium.edu.my/38508/1/May_Shahida_JPAM.pdf http://irep.iium.edu.my/38508/3/38508_Muscle%20Creatine%20Kinase%20%28M-CK%29_SCOPUS.pdf |
| first_indexed |
2023-09-18T20:55:20Z |
| last_indexed |
2023-09-18T20:55:20Z |
| _version_ |
1777410266616561664 |