Receptor mechanisms of Thymoquinone-induced hypotension in normotensive rats

Nigella sativa seeds “black cumin” have been widely used in traditional medicine for diseases treatment including hypertension. Thymoquinone (TQ) is one of the major active constituents in its volatile oil. The objective of the current study was to confirm the blood pressure lowering effect of TQ, a...

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Main Authors:	Mizher, Hussam A., Mohd. Noor, Noriah, Azzubaidi, Marwan Saad
Format:	Article
Language:	English
Published:	MDPI 2014
Subjects:	QP Physiology R Medicine (General) RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/38818/ http://irep.iium.edu.my/38818/ http://irep.iium.edu.my/38818/ http://irep.iium.edu.my/38818/1/Azzubaidi.pdf

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recordtype	eprints
spelling	iium-388182018-05-24T07:59:48Z http://irep.iium.edu.my/38818/ Receptor mechanisms of Thymoquinone-induced hypotension in normotensive rats Mizher, Hussam A. Mohd. Noor, Noriah Azzubaidi, Marwan Saad QP Physiology R Medicine (General) RM Therapeutics. Pharmacology Nigella sativa seeds “black cumin” have been widely used in traditional medicine for diseases treatment including hypertension. Thymoquinone (TQ) is one of the major active constituents in its volatile oil. The objective of the current study was to confirm the blood pressure lowering effect of TQ, and to investigate its mechanism through muscarinic and β adrenergic receptors. Mean arterial blood pressure (MAP) was recorded using the non-invasive blood pressure tail cuff technique. Dose-response relationship was obtained after using 3 TQ doses (2.5, 5 and 10 mg/kg) intraperitoneally to 3 different groups (n = 5) of adult male Sprague Dawley rats under pentobarbital anesthesia. MAP was then measured for other 2 animal groups pretreated either with atropine (P-at) or propranolol (P-pro) followed by 10 mg/kg TQ. TQ produced a dose-dependent blood pressure lowering effect, where 2.5 mg/kg reduced MAP by 8 ± 1 mmHg, whereas 5 and 10 mg/kg of TQ treatment decreased MAP by 12 ± 3 and 29 ± 3 mmHg, respectively. TQ-induced MAP reduction was significantly less in P-at than non-pretreated group. Conversely, TQ-induced MAP reduction in P-pro did not demonstrate significant difference from the non-pretreated group. This study confirms the dose-related hypotensive effect of TQ and provides an evidence for the traditional use of Nigella sativa for treatment of hypertension. The mechanism of TQ-induced hypotensioninvolves at least in part activation of vascular muscarinic receptors, but not β-adrenergic receptors. MDPI 2014-10-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38818/1/Azzubaidi.pdf Mizher, Hussam A. and Mohd. Noor, Noriah and Azzubaidi, Marwan Saad (2014) Receptor mechanisms of Thymoquinone-induced hypotension in normotensive rats. Nutrients, 6 (10). pp. 4149-4150. ISSN 2072-6643 http://www.mdpi.com/2072-6643/6/10/4115/htm 10.3390/nu6104115
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QP Physiology R Medicine (General) RM Therapeutics. Pharmacology
spellingShingle	QP Physiology R Medicine (General) RM Therapeutics. Pharmacology Mizher, Hussam A. Mohd. Noor, Noriah Azzubaidi, Marwan Saad Receptor mechanisms of Thymoquinone-induced hypotension in normotensive rats
description	Nigella sativa seeds “black cumin” have been widely used in traditional medicine for diseases treatment including hypertension. Thymoquinone (TQ) is one of the major active constituents in its volatile oil. The objective of the current study was to confirm the blood pressure lowering effect of TQ, and to investigate its mechanism through muscarinic and β adrenergic receptors. Mean arterial blood pressure (MAP) was recorded using the non-invasive blood pressure tail cuff technique. Dose-response relationship was obtained after using 3 TQ doses (2.5, 5 and 10 mg/kg) intraperitoneally to 3 different groups (n = 5) of adult male Sprague Dawley rats under pentobarbital anesthesia. MAP was then measured for other 2 animal groups pretreated either with atropine (P-at) or propranolol (P-pro) followed by 10 mg/kg TQ. TQ produced a dose-dependent blood pressure lowering effect, where 2.5 mg/kg reduced MAP by 8 ± 1 mmHg, whereas 5 and 10 mg/kg of TQ treatment decreased MAP by 12 ± 3 and 29 ± 3 mmHg, respectively. TQ-induced MAP reduction was significantly less in P-at than non-pretreated group. Conversely, TQ-induced MAP reduction in P-pro did not demonstrate significant difference from the non-pretreated group. This study confirms the dose-related hypotensive effect of TQ and provides an evidence for the traditional use of Nigella sativa for treatment of hypertension. The mechanism of TQ-induced hypotensioninvolves at least in part activation of vascular muscarinic receptors, but not β-adrenergic receptors.
format	Article
author	Mizher, Hussam A. Mohd. Noor, Noriah Azzubaidi, Marwan Saad
author_facet	Mizher, Hussam A. Mohd. Noor, Noriah Azzubaidi, Marwan Saad
author_sort	Mizher, Hussam A.
title	Receptor mechanisms of Thymoquinone-induced hypotension in normotensive rats
title_short	Receptor mechanisms of Thymoquinone-induced hypotension in normotensive rats
title_full	Receptor mechanisms of Thymoquinone-induced hypotension in normotensive rats
title_fullStr	Receptor mechanisms of Thymoquinone-induced hypotension in normotensive rats
title_full_unstemmed	Receptor mechanisms of Thymoquinone-induced hypotension in normotensive rats
title_sort	receptor mechanisms of thymoquinone-induced hypotension in normotensive rats
publisher	MDPI
publishDate	2014
url	http://irep.iium.edu.my/38818/ http://irep.iium.edu.my/38818/ http://irep.iium.edu.my/38818/ http://irep.iium.edu.my/38818/1/Azzubaidi.pdf
first_indexed	2023-09-18T20:55:46Z
last_indexed	2023-09-18T20:55:46Z
_version_	1777410293535604736

Receptor mechanisms of Thymoquinone-induced hypotension in normotensive rats

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