Anti-proliferative bacteriocins active against MRSA from coagulase negative Ent. mundtii strain C4L10 isolated from non-broiler chicken
Bacteriocins produced by a variety of lactic acid bacteria (LAB) are gaining more attention for not only as alternative food preservative but also as therapeutics agent. A bacteriocin, Ent C4L10, was produced by coagulase negative Enterococcus mundtii strain C4L10 (Accession No. KC731423) previously...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
OMICS Group
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/39055/ http://irep.iium.edu.my/39055/ http://irep.iium.edu.my/39055/ http://irep.iium.edu.my/39055/1/haziyamin.pdf |
| Summary: | Bacteriocins produced by a variety of lactic acid bacteria (LAB) are gaining more attention for not only as alternative food preservative but also as therapeutics agent. A bacteriocin, Ent C4L10, was produced by coagulase negative Enterococcus mundtii strain C4L10 (Accession No. KC731423) previously isolated probiotic from Malaysian non-broiler chicken. Based on agar diffusion assay, it showed antimicrobial activities against Methicillin resistance Staphylococcus aureus (MRSA) used as an indicator organism. Approximately 10 kDa protein was purified employing three-phase partitioning (TPP) method and it was shown to be highly thermostable, retaining activities at 121°C for 15min, and was stable in a pH range of 4-9. There was however a loss in activity after protease treatment. PCR amplification using enterocin gene primers showed that Ent C4L10 sequence is highly similar to Bacteriocin L-1077 (83% identity). In order to study its anti-proliferative potential, purified Ent C4L10 was also tested against four human cell lines; i.e., lung cancer (H1299), breast cancer (MCF 7), colon cancer (HCT116) and oral cancer (HSC3). It was found that oral cancer cell line was the most sensitive with a cytotoxic index of IC50 of 9.009µg/ml, followed by breast cancer (IC50 (11.51µg/ml), and the least sensitive was with colon cancer cell line (IC50 of 20.57µg/ml). In conclusion, putative 10 KDa Ent C4L10 is a class IIa bacteriocin isolated from coagulase negative Enterococcus mundtii strain C4L10 shown to have anti-proliferative properties. Therefore, this bacteriocin has not only great potential for use in food preservation, it future use as an antitumor agent should also be explored |
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