Dynamics behavior of Endoglucanase from Fusarium oxysporum

Molecular dynamics methods are very useful tool in understanding the behavior of the enzymes at higher temperatures. It is well known that some enzymes lose their enzyme activity at higher temperatures. In this work we employ molecular dynamics simulation of an endoglucanase from Fusarium oxyspor...

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Main Authors:	Noorbatcha, Ibrahim Ali, S., Waesoho, Mohd. Salleh, Hamzah
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/3911/ http://irep.iium.edu.my/3911/ http://irep.iium.edu.my/3911/1/Dyn_Behavr_of_EG_from_F_oxysporum_pg286-288.pdf

id	iium-3911
recordtype	eprints
spelling	iium-39112011-11-16T02:19:09Z http://irep.iium.edu.my/3911/ Dynamics behavior of Endoglucanase from Fusarium oxysporum Noorbatcha, Ibrahim Ali S., Waesoho Mohd. Salleh, Hamzah TP248.13 Biotechnology Molecular dynamics methods are very useful tool in understanding the behavior of the enzymes at higher temperatures. It is well known that some enzymes lose their enzyme activity at higher temperatures. In this work we employ molecular dynamics simulation of an endoglucanase from Fusarium oxysporum (EGFO) to understand the reasons behind the loss of enzyme activity when the temperatures is increased form 40°C to 80°C. The dynamic behavior of this enzyme is analyzed by examining the Root Mean Square Derivation(RMSD), salt bridges, gyration ratio and hydrogen bonds at different temperatures as a function of time. It has been found that there are differences in (i) the RMSD of backbone and coil regions, (ii) the number of hydrogen bonds among amino acidresidues and between amino acid residues and explicit watermolecules within the coil region of the enzyme (iii) total number of salt bridges, and (iv) the gyration ratio of the enzyme at different temperatures. These factors could play significant role in the loss of activity of EGFO as the temperature is increased. 2011-05-17 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/3911/1/Dyn_Behavr_of_EG_from_F_oxysporum_pg286-288.pdf Noorbatcha, Ibrahim Ali and S., Waesoho and Mohd. Salleh, Hamzah (2011) Dynamics behavior of Endoglucanase from Fusarium oxysporum. In: International Conference on Biotechnology Engineering, ICBioE’11, 17-19 May, 2011 , Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Noorbatcha, Ibrahim Ali S., Waesoho Mohd. Salleh, Hamzah Dynamics behavior of Endoglucanase from Fusarium oxysporum
description	Molecular dynamics methods are very useful tool in understanding the behavior of the enzymes at higher temperatures. It is well known that some enzymes lose their enzyme activity at higher temperatures. In this work we employ molecular dynamics simulation of an endoglucanase from Fusarium oxysporum (EGFO) to understand the reasons behind the loss of enzyme activity when the temperatures is increased form 40°C to 80°C. The dynamic behavior of this enzyme is analyzed by examining the Root Mean Square Derivation(RMSD), salt bridges, gyration ratio and hydrogen bonds at different temperatures as a function of time. It has been found that there are differences in (i) the RMSD of backbone and coil regions, (ii) the number of hydrogen bonds among amino acidresidues and between amino acid residues and explicit watermolecules within the coil region of the enzyme (iii) total number of salt bridges, and (iv) the gyration ratio of the enzyme at different temperatures. These factors could play significant role in the loss of activity of EGFO as the temperature is increased.
format	Conference or Workshop Item
author	Noorbatcha, Ibrahim Ali S., Waesoho Mohd. Salleh, Hamzah
author_facet	Noorbatcha, Ibrahim Ali S., Waesoho Mohd. Salleh, Hamzah
author_sort	Noorbatcha, Ibrahim Ali
title	Dynamics behavior of Endoglucanase from Fusarium oxysporum
title_short	Dynamics behavior of Endoglucanase from Fusarium oxysporum
title_full	Dynamics behavior of Endoglucanase from Fusarium oxysporum
title_fullStr	Dynamics behavior of Endoglucanase from Fusarium oxysporum
title_full_unstemmed	Dynamics behavior of Endoglucanase from Fusarium oxysporum
title_sort	dynamics behavior of endoglucanase from fusarium oxysporum
publishDate	2011
url	http://irep.iium.edu.my/3911/ http://irep.iium.edu.my/3911/ http://irep.iium.edu.my/3911/1/Dyn_Behavr_of_EG_from_F_oxysporum_pg286-288.pdf
first_indexed	2023-09-18T20:11:54Z
last_indexed	2023-09-18T20:11:54Z
_version_	1777407533352222720

Dynamics behavior of Endoglucanase from Fusarium oxysporum

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