$Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis$

Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis

A lipid acyl hydrolase (LAH) enzyme has been purified from skim latex serum, a waste product of Hevea brasiliensis, using DEAE Sepharose™ Fast Flow ion exchange chromatography. The ~45kDa active LAH was expressed by spectrophotometric study and SDS-PAGE. One-Factor-at-A-Time (OFAT) method and Centra...

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Main Authors:	Mohamed, Nazhirah, Yusof, Faridah
Format:	Article
Language:	English English
Published:	AENSI Publications 2014
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/39221/ http://irep.iium.edu.my/39221/ http://irep.iium.edu.my/39221/1/P_46.pdf http://irep.iium.edu.my/39221/4/39221_Experimental%20design%20and%20statistical_scopus.pdf

id	iium-39221
recordtype	eprints
spelling	iium-392212017-09-06T01:54:46Z http://irep.iium.edu.my/39221/ Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis Mohamed, Nazhirah Yusof, Faridah TP248.13 Biotechnology A lipid acyl hydrolase (LAH) enzyme has been purified from skim latex serum, a waste product of Hevea brasiliensis, using DEAE Sepharose™ Fast Flow ion exchange chromatography. The ~45kDa active LAH was expressed by spectrophotometric study and SDS-PAGE. One-Factor-at-A-Time (OFAT) method and Central Composite Design (CCD) in Response Surface Methodology (RSM) were employed to optimize the protein buffer in order to improve purification. Design Expert® 6.0.8. software was applied using a 2-factor, 5-level to find the optimum pH and concentration of protein buffer to yield the highest lipase acyl hydrolase. In this study, it is concluded that purification at pH 8.0, 20 mM Tris-HCl buffer on ion exchange column chromatography results in the production of the highest total LAH activity (61.152 U). The study has good significance in that it is an initial attempt to purify LAH enzyme, which has known as antifungal agent, from skim latex. AENSI Publications 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/39221/1/P_46.pdf application/pdf en http://irep.iium.edu.my/39221/4/39221_Experimental%20design%20and%20statistical_scopus.pdf Mohamed, Nazhirah and Yusof, Faridah (2014) Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis. Advances in Environmental Biology, 8 (3). pp. 672-679. ISSN 1995-0756 E-ISSN 1998-1066 http://www.aensiweb.com/old/aeb_Special_2014.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Mohamed, Nazhirah Yusof, Faridah Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis
description	A lipid acyl hydrolase (LAH) enzyme has been purified from skim latex serum, a waste product of Hevea brasiliensis, using DEAE Sepharose™ Fast Flow ion exchange chromatography. The ~45kDa active LAH was expressed by spectrophotometric study and SDS-PAGE. One-Factor-at-A-Time (OFAT) method and Central Composite Design (CCD) in Response Surface Methodology (RSM) were employed to optimize the protein buffer in order to improve purification. Design Expert® 6.0.8. software was applied using a 2-factor, 5-level to find the optimum pH and concentration of protein buffer to yield the highest lipase acyl hydrolase. In this study, it is concluded that purification at pH 8.0, 20 mM Tris-HCl buffer on ion exchange column chromatography results in the production of the highest total LAH activity (61.152 U). The study has good significance in that it is an initial attempt to purify LAH enzyme, which has known as antifungal agent, from skim latex.
format	Article
author	Mohamed, Nazhirah Yusof, Faridah
author_facet	Mohamed, Nazhirah Yusof, Faridah
author_sort	Mohamed, Nazhirah
title	Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis
title_short	Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis
title_full	Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis
title_fullStr	Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis
title_full_unstemmed	Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis
title_sort	experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis
publisher	AENSI Publications
publishDate	2014
url	http://irep.iium.edu.my/39221/ http://irep.iium.edu.my/39221/ http://irep.iium.edu.my/39221/1/P_46.pdf http://irep.iium.edu.my/39221/4/39221_Experimental%20design%20and%20statistical_scopus.pdf
first_indexed	2023-09-18T20:56:19Z
last_indexed	2023-09-18T20:56:19Z
_version_	1777410328159584256

Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis

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