Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis

A lipid acyl hydrolase (LAH) enzyme has been purified from skim latex serum, a waste product of Hevea brasiliensis, using DEAE Sepharoseâ„¢ Fast Flow ion exchange chromatography. The ~45kDa active LAH was expressed by spectrophotometric study and SDS-PAGE. One-Factor-at-A-Time (OFAT) method and Centra...

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Bibliographic Details
Main Authors: Mohamed, Nazhirah, Yusof, Faridah
Format: Article
Language:English
English
Published: AENSI Publications 2014
Subjects:
Online Access:http://irep.iium.edu.my/39221/
http://irep.iium.edu.my/39221/
http://irep.iium.edu.my/39221/1/P_46.pdf
http://irep.iium.edu.my/39221/4/39221_Experimental%20design%20and%20statistical_scopus.pdf

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