Expression of segment a of infectious bursal disease virus in pichia pastoris

Recombinant plasmid containing segment A open reading frame 2 (ORF2) gene of infectious bursal disease virus (IBDV) of a very virulent subtype from local outbreak (strain 3529/92) was constructed. The gene encoding the IBDV structural polyprotein (N-VP2-VP3-VP4-C) was inserted into an expression vec...

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Main Authors:	Ghazali, Mohd. Shahrin, Ahmad Raus, Raha, Amin, Noriha M., Shohaimi, Syamsiah A., Abu Bakar, Farah D., Zainuddin, Norafiza, Jimat, Dzun Noraini, Nor Muhammad, Nor Azlin Alia
Format:	Article
Language:	English English
Published:	Journal of Pure and Applied Microbiology 2014
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/39365/ http://irep.iium.edu.my/39365/ http://irep.iium.edu.my/39365/2/Expression_of_Segment_A_gene.pdf http://irep.iium.edu.my/39365/4/39365_Expression%20of%20segment_Scopus.pdf

id	iium-39365
recordtype	eprints
spelling	iium-393652018-06-12T03:41:58Z http://irep.iium.edu.my/39365/ Expression of segment a of infectious bursal disease virus in pichia pastoris Ghazali, Mohd. Shahrin Ahmad Raus, Raha Amin, Noriha M. Shohaimi, Syamsiah A. Abu Bakar, Farah D. Zainuddin, Norafiza Jimat, Dzun Noraini Nor Muhammad, Nor Azlin Alia Q Science (General) Recombinant plasmid containing segment A open reading frame 2 (ORF2) gene of infectious bursal disease virus (IBDV) of a very virulent subtype from local outbreak (strain 3529/92) was constructed. The gene encoding the IBDV structural polyprotein (N-VP2-VP3-VP4-C) was inserted into an expression vector, pPICZ prior to its transformation into Pichia pastoris by electroporation. After the induction of P. pastoris transformant with 0.5% methanol, the production of IBDV polyprotein was observed using Western blot. In P. pastoris, co- or post-translational processing of the large polyprotein occurred, generating a stable C-terminal product (VP3) of correct size, but without any detectable N-terminal product (VP2). The failure to observe the VP2 protein in Western blot analysis was probably due to the conformational epitope problem. Journal of Pure and Applied Microbiology 2014-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/39365/2/Expression_of_Segment_A_gene.pdf application/pdf en http://irep.iium.edu.my/39365/4/39365_Expression%20of%20segment_Scopus.pdf Ghazali, Mohd. Shahrin and Ahmad Raus, Raha and Amin, Noriha M. and Shohaimi, Syamsiah A. and Abu Bakar, Farah D. and Zainuddin, Norafiza and Jimat, Dzun Noraini and Nor Muhammad, Nor Azlin Alia (2014) Expression of segment a of infectious bursal disease virus in pichia pastoris. Journal of Pure and Applied Microbiology, 8 spl. pp. 875-879. ISSN 0973-7510 http://www.microbiologyjournal.org/archive.php
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Ghazali, Mohd. Shahrin Ahmad Raus, Raha Amin, Noriha M. Shohaimi, Syamsiah A. Abu Bakar, Farah D. Zainuddin, Norafiza Jimat, Dzun Noraini Nor Muhammad, Nor Azlin Alia Expression of segment a of infectious bursal disease virus in pichia pastoris
description	Recombinant plasmid containing segment A open reading frame 2 (ORF2) gene of infectious bursal disease virus (IBDV) of a very virulent subtype from local outbreak (strain 3529/92) was constructed. The gene encoding the IBDV structural polyprotein (N-VP2-VP3-VP4-C) was inserted into an expression vector, pPICZ prior to its transformation into Pichia pastoris by electroporation. After the induction of P. pastoris transformant with 0.5% methanol, the production of IBDV polyprotein was observed using Western blot. In P. pastoris, co- or post-translational processing of the large polyprotein occurred, generating a stable C-terminal product (VP3) of correct size, but without any detectable N-terminal product (VP2). The failure to observe the VP2 protein in Western blot analysis was probably due to the conformational epitope problem.
format	Article
author	Ghazali, Mohd. Shahrin Ahmad Raus, Raha Amin, Noriha M. Shohaimi, Syamsiah A. Abu Bakar, Farah D. Zainuddin, Norafiza Jimat, Dzun Noraini Nor Muhammad, Nor Azlin Alia
author_facet	Ghazali, Mohd. Shahrin Ahmad Raus, Raha Amin, Noriha M. Shohaimi, Syamsiah A. Abu Bakar, Farah D. Zainuddin, Norafiza Jimat, Dzun Noraini Nor Muhammad, Nor Azlin Alia
author_sort	Ghazali, Mohd. Shahrin
title	Expression of segment a of infectious bursal disease virus in pichia pastoris
title_short	Expression of segment a of infectious bursal disease virus in pichia pastoris
title_full	Expression of segment a of infectious bursal disease virus in pichia pastoris
title_fullStr	Expression of segment a of infectious bursal disease virus in pichia pastoris
title_full_unstemmed	Expression of segment a of infectious bursal disease virus in pichia pastoris
title_sort	expression of segment a of infectious bursal disease virus in pichia pastoris
publisher	Journal of Pure and Applied Microbiology
publishDate	2014
url	http://irep.iium.edu.my/39365/ http://irep.iium.edu.my/39365/ http://irep.iium.edu.my/39365/2/Expression_of_Segment_A_gene.pdf http://irep.iium.edu.my/39365/4/39365_Expression%20of%20segment_Scopus.pdf
first_indexed	2023-09-18T20:56:33Z
last_indexed	2023-09-18T20:56:33Z
_version_	1777410342916194304

Expression of segment a of infectious bursal disease virus in pichia pastoris

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