Comparison studies of GSH isolation from several Malaysian local fruits by mechanical methods

Glutathione (GSH) is a simple tripeptide produced by the liver. It is a volatile substance, which is sweet in taste, comprised of the amino acids cysteine, glutamic acid and glycine. In these modern days, the use of traditional medicine by the suffers of chronic disease is encouraged due to the adve...

Full description

Bibliographic Details
Main Authors:	Baiti, Arif Afraha, Jamal , Parveen, Mirghani, Mohamed Elwathig Saeed
Format:	Article
Language:	English English
Published:	Research Journal of Chemistry and Environment 2010
Subjects:	R Medicine (General) TP Chemical technology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/3937/ http://irep.iium.edu.my/3937/ http://irep.iium.edu.my/3937/2/FullpaperICCE2009-Parveen%2C_Afraha__299-302%2C_special_issue.pdf http://irep.iium.edu.my/3937/1/Front_Cover.pdf

id	iium-3937
recordtype	eprints
spelling	iium-39372012-01-24T09:53:17Z http://irep.iium.edu.my/3937/ Comparison studies of GSH isolation from several Malaysian local fruits by mechanical methods Baiti, Arif Afraha Jamal , Parveen Mirghani, Mohamed Elwathig Saeed R Medicine (General) TP Chemical technology TP248.13 Biotechnology Glutathione (GSH) is a simple tripeptide produced by the liver. It is a volatile substance, which is sweet in taste, comprised of the amino acids cysteine, glutamic acid and glycine. In these modern days, the use of traditional medicine by the suffers of chronic disease is encouraged due to the adverse effects of chemical drugs and treatment using medicines of natural origin appears to offer more gentle means of managing such disease. Therefore, the practice of traditionally used natural plants as medicines is one of the alternative ways to cure the illnesses. Besides, this study was done in order to investigate the production of GSH from fruits since most of the studies that had been conducted before were done on the isolation of GSH from other sources. In this study the reduced glutathione (GSH) was isolated from a potential local fruit of Malaysia. Three mechanical methods; homogenizer, ultrasonic and autolysis were screened for disrupting the fruits cells for maximum GSH content. Different methods of cell disruption gave different effect on the GSH production. Watermelon (Citrullus lanatus) is the best potential local fruit that contain higher GSH followed by Jackfruit (Artocarpus heterophylus) and Sapodilla (Manilkara zapota), while ultrasonic is the best cell disruption method which can give the maximum amount of GSH, followed by homogenizer method and autolysis method. Research Journal of Chemistry and Environment 2010-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/3937/2/FullpaperICCE2009-Parveen%2C_Afraha__299-302%2C_special_issue.pdf application/pdf en http://irep.iium.edu.my/3937/1/Front_Cover.pdf Baiti, Arif Afraha and Jamal , Parveen and Mirghani, Mohamed Elwathig Saeed (2010) Comparison studies of GSH isolation from several Malaysian local fruits by mechanical methods. Research Journal of Chemistry and Environment, Spcial. pp. 299-302. ISSN 0972-0626 http://www.chemenviron.net/RJCE/index.htm
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	R Medicine (General) TP Chemical technology TP248.13 Biotechnology
spellingShingle	R Medicine (General) TP Chemical technology TP248.13 Biotechnology Baiti, Arif Afraha Jamal , Parveen Mirghani, Mohamed Elwathig Saeed Comparison studies of GSH isolation from several Malaysian local fruits by mechanical methods
description	Glutathione (GSH) is a simple tripeptide produced by the liver. It is a volatile substance, which is sweet in taste, comprised of the amino acids cysteine, glutamic acid and glycine. In these modern days, the use of traditional medicine by the suffers of chronic disease is encouraged due to the adverse effects of chemical drugs and treatment using medicines of natural origin appears to offer more gentle means of managing such disease. Therefore, the practice of traditionally used natural plants as medicines is one of the alternative ways to cure the illnesses. Besides, this study was done in order to investigate the production of GSH from fruits since most of the studies that had been conducted before were done on the isolation of GSH from other sources. In this study the reduced glutathione (GSH) was isolated from a potential local fruit of Malaysia. Three mechanical methods; homogenizer, ultrasonic and autolysis were screened for disrupting the fruits cells for maximum GSH content. Different methods of cell disruption gave different effect on the GSH production. Watermelon (Citrullus lanatus) is the best potential local fruit that contain higher GSH followed by Jackfruit (Artocarpus heterophylus) and Sapodilla (Manilkara zapota), while ultrasonic is the best cell disruption method which can give the maximum amount of GSH, followed by homogenizer method and autolysis method.
format	Article
author	Baiti, Arif Afraha Jamal , Parveen Mirghani, Mohamed Elwathig Saeed
author_facet	Baiti, Arif Afraha Jamal , Parveen Mirghani, Mohamed Elwathig Saeed
author_sort	Baiti, Arif Afraha
title	Comparison studies of GSH isolation from several Malaysian local fruits by mechanical methods
title_short	Comparison studies of GSH isolation from several Malaysian local fruits by mechanical methods
title_full	Comparison studies of GSH isolation from several Malaysian local fruits by mechanical methods
title_fullStr	Comparison studies of GSH isolation from several Malaysian local fruits by mechanical methods
title_full_unstemmed	Comparison studies of GSH isolation from several Malaysian local fruits by mechanical methods
title_sort	comparison studies of gsh isolation from several malaysian local fruits by mechanical methods
publisher	Research Journal of Chemistry and Environment
publishDate	2010
url	http://irep.iium.edu.my/3937/ http://irep.iium.edu.my/3937/ http://irep.iium.edu.my/3937/2/FullpaperICCE2009-Parveen%2C_Afraha__299-302%2C_special_issue.pdf http://irep.iium.edu.my/3937/1/Front_Cover.pdf
first_indexed	2023-09-18T20:11:56Z
last_indexed	2023-09-18T20:11:56Z
_version_	1777407535364440064

Comparison studies of GSH isolation from several Malaysian local fruits by mechanical methods

Similar Items