Screening for antibacterial activity of Andrographis paniculata used in Malaysian folkloric medicine: a possible alternative for the treatment of skin infections

In this study non-polar (dichloromethane) and polar (MeOH & aqueous) extracts of A. paniculata (whole plant) were evaluated for in vitro antibacterial activity against 12 skin disease causing bacterial strains (7 gram positive strains; Staphylococcus saprophyticus, Staphylococcus epidermis, Sta...

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Main Authors:	Sule, Abubakar, Ahmed, Qamar Uddin, Abd. Samah, Othman, Omar, Muhammad Nor
Format:	Article
Language:	English
Published:	Southern Illinois University Carbondale, USA. 2010
Subjects:	RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/3942/ http://irep.iium.edu.my/3942/ http://irep.iium.edu.my/3942/2/Ethnobotanical_Leaflets_14.pdf

id	iium-3942
recordtype	eprints
spelling	iium-39422011-11-22T04:17:55Z http://irep.iium.edu.my/3942/ Screening for antibacterial activity of Andrographis paniculata used in Malaysian folkloric medicine: a possible alternative for the treatment of skin infections Sule, Abubakar Ahmed, Qamar Uddin Abd. Samah, Othman Omar, Muhammad Nor RM Therapeutics. Pharmacology In this study non-polar (dichloromethane) and polar (MeOH & aqueous) extracts of A. paniculata (whole plant) were evaluated for in vitro antibacterial activity against 12 skin disease causing bacterial strains (7 gram positive strains; Staphylococcus saprophyticus, Staphylococcus epidermis, Staphylococcus aureus, Streptococcus pyogenes, Bacillus anthracis, Micrococcus luteus, Enterococcus faecalis) and 5 gram negative strains; Proteus mirabilis, Proteus vulgaris, Klebsiella pneumoniae, Neisseria meningitis, Pseudomonas aeruginosa ) using the disc diffusion method at three concentrations; 1000, 500, and 250 µg/disc respectively in order to ascertain its folkloric claim to treat skin infections. The extracts showed significant antibacterial activities against both the Gram-positive and Gram-negative bacterial strains tested. Highest significant antibacterial activity was exerted by the MeOH extract against E. faecalis at 1000 µg/disc (24.00 ± 0.00 mm) and the least activity by the DCM extract against N. meningitis at 250 µg/disc (6.00 ± 0.00mm). The minimum inhibitory concentration ranged between 150 µg /mL and 300 µg /mL depending on microorganism and various extracts. Presence of phytochemicals such as terpenoids, tannins, flavonoids, saponins, alkaloids, amino acids and steroids were observed. These results candidly suggest the presence of promising antibacterial substances in the polar as well as non-polar extracts which could be potential phytomedicine for the treatment of skin infections caused by pathogenic bacterial strains. These findings explicitly support its traditional claims and form a strong basis for further efforts to explore A. paniculata’s antibacterial potential to treat skin frailties efficaciously. Our results confer the utility of this plant extracts in developing a novel broad spectrum antimicrobial agent. Southern Illinois University Carbondale, USA. 2010-04-01 Article PeerReviewed application/pdf en cc_by_nc http://irep.iium.edu.my/3942/2/Ethnobotanical_Leaflets_14.pdf Sule, Abubakar and Ahmed, Qamar Uddin and Abd. Samah, Othman and Omar, Muhammad Nor (2010) Screening for antibacterial activity of Andrographis paniculata used in Malaysian folkloric medicine: a possible alternative for the treatment of skin infections. Ethnobotanical Leaflets, 14 (-). pp. 445-456. ISSN 1948-3570 http://www.ethnoleaflets.com/leaflets/malayan.htm
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM Therapeutics. Pharmacology
spellingShingle	RM Therapeutics. Pharmacology Sule, Abubakar Ahmed, Qamar Uddin Abd. Samah, Othman Omar, Muhammad Nor Screening for antibacterial activity of Andrographis paniculata used in Malaysian folkloric medicine: a possible alternative for the treatment of skin infections
description	In this study non-polar (dichloromethane) and polar (MeOH & aqueous) extracts of A. paniculata (whole plant) were evaluated for in vitro antibacterial activity against 12 skin disease causing bacterial strains (7 gram positive strains; Staphylococcus saprophyticus, Staphylococcus epidermis, Staphylococcus aureus, Streptococcus pyogenes, Bacillus anthracis, Micrococcus luteus, Enterococcus faecalis) and 5 gram negative strains; Proteus mirabilis, Proteus vulgaris, Klebsiella pneumoniae, Neisseria meningitis, Pseudomonas aeruginosa ) using the disc diffusion method at three concentrations; 1000, 500, and 250 µg/disc respectively in order to ascertain its folkloric claim to treat skin infections. The extracts showed significant antibacterial activities against both the Gram-positive and Gram-negative bacterial strains tested. Highest significant antibacterial activity was exerted by the MeOH extract against E. faecalis at 1000 µg/disc (24.00 ± 0.00 mm) and the least activity by the DCM extract against N. meningitis at 250 µg/disc (6.00 ± 0.00mm). The minimum inhibitory concentration ranged between 150 µg /mL and 300 µg /mL depending on microorganism and various extracts. Presence of phytochemicals such as terpenoids, tannins, flavonoids, saponins, alkaloids, amino acids and steroids were observed. These results candidly suggest the presence of promising antibacterial substances in the polar as well as non-polar extracts which could be potential phytomedicine for the treatment of skin infections caused by pathogenic bacterial strains. These findings explicitly support its traditional claims and form a strong basis for further efforts to explore A. paniculata’s antibacterial potential to treat skin frailties efficaciously. Our results confer the utility of this plant extracts in developing a novel broad spectrum antimicrobial agent.
format	Article
author	Sule, Abubakar Ahmed, Qamar Uddin Abd. Samah, Othman Omar, Muhammad Nor
author_facet	Sule, Abubakar Ahmed, Qamar Uddin Abd. Samah, Othman Omar, Muhammad Nor
author_sort	Sule, Abubakar
title	Screening for antibacterial activity of Andrographis paniculata used in Malaysian folkloric medicine: a possible alternative for the treatment of skin infections
title_short	Screening for antibacterial activity of Andrographis paniculata used in Malaysian folkloric medicine: a possible alternative for the treatment of skin infections
title_full	Screening for antibacterial activity of Andrographis paniculata used in Malaysian folkloric medicine: a possible alternative for the treatment of skin infections
title_fullStr	Screening for antibacterial activity of Andrographis paniculata used in Malaysian folkloric medicine: a possible alternative for the treatment of skin infections
title_full_unstemmed	Screening for antibacterial activity of Andrographis paniculata used in Malaysian folkloric medicine: a possible alternative for the treatment of skin infections
title_sort	screening for antibacterial activity of andrographis paniculata used in malaysian folkloric medicine: a possible alternative for the treatment of skin infections
publisher	Southern Illinois University Carbondale, USA.
publishDate	2010
url	http://irep.iium.edu.my/3942/ http://irep.iium.edu.my/3942/ http://irep.iium.edu.my/3942/2/Ethnobotanical_Leaflets_14.pdf
first_indexed	2023-09-18T20:11:56Z
last_indexed	2023-09-18T20:11:56Z
_version_	1777407535818473472

Screening for antibacterial activity of Andrographis paniculata used in Malaysian folkloric medicine: a possible alternative for the treatment of skin infections

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