Impact of microbial transformation metabolome of Asiaticoside on wound healing

Microbial transformation has been extensively used, to create new and useful metabolites. Microbial transformations can primarily be used in engineering and altering the effective constituents of the phytomedicine, increasing the natural products collection, and to draw the routes of drugs’ synthesi...

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Main Authors:	Alfarra, Helmi Yousif, Ichwan, Solachuddin JA, Omar, Muhammad Nor
Format:	Article
Language:	English
Published:	Tissue Engineering and Regenerative Medicine Society of Malaysia 2014
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/39489/ http://irep.iium.edu.my/39489/ http://irep.iium.edu.my/39489/ http://irep.iium.edu.my/39489/1/Helmi_Regenerative_research.pdf

id	iium-39489
recordtype	eprints
spelling	iium-394892018-05-24T01:32:54Z http://irep.iium.edu.my/39489/ Impact of microbial transformation metabolome of Asiaticoside on wound healing Alfarra, Helmi Yousif Ichwan, Solachuddin JA Omar, Muhammad Nor RS Pharmacy and materia medica Microbial transformation has been extensively used, to create new and useful metabolites. Microbial transformations can primarily be used in engineering and altering the effective constituents of the phytomedicine, increasing the natural products collection, and to draw the routes of drugs’ synthesis. Wound healing process normally starts with three subsequent actions that may be shared in period and area; these complicated stages include the inflammation phase, proliferation or tissue formation phase and remodeling phase. Asiaticoside was isolated from Centella asiatica and exposed to the microbial catalysis and then the metabolites were extracted. The effects of asiaticoside and its transformed products were investigated on human skin cells using human keratinocyte Hacat cell line as the in vitro model. In wound scratch assay on Hacat cells, asiaticoside and its microbial metabolome was shown to increase the migration rates of the cells. By tracking the area occupied by the cells and the number of cells attached, we could conclude that asiaticoside with its biocatalysed components increased the initial Hacat cell migration and adhesion. It was observed that the microbial metabolome demonstrated its effect with minimal concentration than its original compound. In cell proliferation assays, asiaticoside and the metabolome showed their efficacy in inducing the increase of cell behaviors. It can be assumed that the bioactivity on artificial skin, might suggest them to have pharmaceutical values in addition to the healing significance. Tissue Engineering and Regenerative Medicine Society of Malaysia 2014-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/39489/1/Helmi_Regenerative_research.pdf Alfarra, Helmi Yousif and Ichwan, Solachuddin JA and Omar, Muhammad Nor (2014) Impact of microbial transformation metabolome of Asiaticoside on wound healing. Regenerative Research, 3 (2). pp. 76-77. ISSN 2232-0822 (Online) http://www.regres.tesma.org.my http://regres.tesma.org.my/pdf/RR-181114-047.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Alfarra, Helmi Yousif Ichwan, Solachuddin JA Omar, Muhammad Nor Impact of microbial transformation metabolome of Asiaticoside on wound healing
description	Microbial transformation has been extensively used, to create new and useful metabolites. Microbial transformations can primarily be used in engineering and altering the effective constituents of the phytomedicine, increasing the natural products collection, and to draw the routes of drugs’ synthesis. Wound healing process normally starts with three subsequent actions that may be shared in period and area; these complicated stages include the inflammation phase, proliferation or tissue formation phase and remodeling phase. Asiaticoside was isolated from Centella asiatica and exposed to the microbial catalysis and then the metabolites were extracted. The effects of asiaticoside and its transformed products were investigated on human skin cells using human keratinocyte Hacat cell line as the in vitro model. In wound scratch assay on Hacat cells, asiaticoside and its microbial metabolome was shown to increase the migration rates of the cells. By tracking the area occupied by the cells and the number of cells attached, we could conclude that asiaticoside with its biocatalysed components increased the initial Hacat cell migration and adhesion. It was observed that the microbial metabolome demonstrated its effect with minimal concentration than its original compound. In cell proliferation assays, asiaticoside and the metabolome showed their efficacy in inducing the increase of cell behaviors. It can be assumed that the bioactivity on artificial skin, might suggest them to have pharmaceutical values in addition to the healing significance.
format	Article
author	Alfarra, Helmi Yousif Ichwan, Solachuddin JA Omar, Muhammad Nor
author_facet	Alfarra, Helmi Yousif Ichwan, Solachuddin JA Omar, Muhammad Nor
author_sort	Alfarra, Helmi Yousif
title	Impact of microbial transformation metabolome of Asiaticoside on wound healing
title_short	Impact of microbial transformation metabolome of Asiaticoside on wound healing
title_full	Impact of microbial transformation metabolome of Asiaticoside on wound healing
title_fullStr	Impact of microbial transformation metabolome of Asiaticoside on wound healing
title_full_unstemmed	Impact of microbial transformation metabolome of Asiaticoside on wound healing
title_sort	impact of microbial transformation metabolome of asiaticoside on wound healing
publisher	Tissue Engineering and Regenerative Medicine Society of Malaysia
publishDate	2014
url	http://irep.iium.edu.my/39489/ http://irep.iium.edu.my/39489/ http://irep.iium.edu.my/39489/ http://irep.iium.edu.my/39489/1/Helmi_Regenerative_research.pdf
first_indexed	2023-09-18T20:56:44Z
last_indexed	2023-09-18T20:56:44Z
_version_	1777410354258640896

Impact of microbial transformation metabolome of Asiaticoside on wound healing

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