Cellular uptake of nigella sativa oil-PLGA microparticle by PC-12 cell line

The aim of this study is to investigate the cell uptake of Nigella sativa oil (NSO)-PLGA microparticle by neuron-like PC-12 cells in comparison to surfactants; hydrophilic (Tween 80 & Triton X100) and hydrophobic (Span 80). Solvent evaporation was used to precisely control the size, zeta potenti...

Full description

Bibliographic Details
Main Authors:	Doolaanea, Abd Almonem, Mansor, Nur ‘Izzati, Mohd Nor, Nurul Hafizah, Mohamed, Farahidah
Format:	Article
Language:	English
Published:	Informa UK Ltd 2014
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/39639/ http://irep.iium.edu.my/39639/ http://irep.iium.edu.my/39639/ http://irep.iium.edu.my/39639/2/cellular_uptake.pdf

id	iium-39639
recordtype	eprints
spelling	iium-396392015-03-11T08:11:38Z http://irep.iium.edu.my/39639/ Cellular uptake of nigella sativa oil-PLGA microparticle by PC-12 cell line Doolaanea, Abd Almonem Mansor, Nur ‘Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah RS Pharmacy and materia medica The aim of this study is to investigate the cell uptake of Nigella sativa oil (NSO)-PLGA microparticle by neuron-like PC-12 cells in comparison to surfactants; hydrophilic (Tween 80 & Triton X100) and hydrophobic (Span 80). Solvent evaporation was used to precisely control the size, zeta potential and morphology of the particle. The results revealed varying efficiencies of the cell uptake by PC-12 cells, which may be partially attributed to the surface hydrophobicity of the microparticles. Interestingly, the uptake efficiency of PC-12 cells was higher with the more hydrophilic microparticle. NSO microparticle showed evidence of being preferably internalised by mitotic cells. Tween 80 microparticle showed the highest cell uptake efficiency with a concentration-dependent pattern suggesting its use as uptake enhancer for non-scavenging cells. In conclusion, PC-12 cells can take up NSO-PLGA microparticle which may have potential in the treatment of neurodegenerative disease. Informa UK Ltd 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/39639/2/cellular_uptake.pdf Doolaanea, Abd Almonem and Mansor, Nur ‘Izzati and Mohd Nor, Nurul Hafizah and Mohamed, Farahidah (2014) Cellular uptake of nigella sativa oil-PLGA microparticle by PC-12 cell line. Journal of Microencapsulation, 31 (6). pp. 600-608. ISSN 0265-2048 (P), 1464-5246 (O) http://dx.doi.org/10.3109/02652048.2014.898709 doi:10.3109/02652048.2014.898709
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Doolaanea, Abd Almonem Mansor, Nur ‘Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah Cellular uptake of nigella sativa oil-PLGA microparticle by PC-12 cell line
description	The aim of this study is to investigate the cell uptake of Nigella sativa oil (NSO)-PLGA microparticle by neuron-like PC-12 cells in comparison to surfactants; hydrophilic (Tween 80 & Triton X100) and hydrophobic (Span 80). Solvent evaporation was used to precisely control the size, zeta potential and morphology of the particle. The results revealed varying efficiencies of the cell uptake by PC-12 cells, which may be partially attributed to the surface hydrophobicity of the microparticles. Interestingly, the uptake efficiency of PC-12 cells was higher with the more hydrophilic microparticle. NSO microparticle showed evidence of being preferably internalised by mitotic cells. Tween 80 microparticle showed the highest cell uptake efficiency with a concentration-dependent pattern suggesting its use as uptake enhancer for non-scavenging cells. In conclusion, PC-12 cells can take up NSO-PLGA microparticle which may have potential in the treatment of neurodegenerative disease.
format	Article
author	Doolaanea, Abd Almonem Mansor, Nur ‘Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah
author_facet	Doolaanea, Abd Almonem Mansor, Nur ‘Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah
author_sort	Doolaanea, Abd Almonem
title	Cellular uptake of nigella sativa oil-PLGA microparticle by PC-12 cell line
title_short	Cellular uptake of nigella sativa oil-PLGA microparticle by PC-12 cell line
title_full	Cellular uptake of nigella sativa oil-PLGA microparticle by PC-12 cell line
title_fullStr	Cellular uptake of nigella sativa oil-PLGA microparticle by PC-12 cell line
title_full_unstemmed	Cellular uptake of nigella sativa oil-PLGA microparticle by PC-12 cell line
title_sort	cellular uptake of nigella sativa oil-plga microparticle by pc-12 cell line
publisher	Informa UK Ltd
publishDate	2014
url	http://irep.iium.edu.my/39639/ http://irep.iium.edu.my/39639/ http://irep.iium.edu.my/39639/ http://irep.iium.edu.my/39639/2/cellular_uptake.pdf
first_indexed	2023-09-18T20:56:58Z
last_indexed	2023-09-18T20:56:58Z
_version_	1777410368517177344

Cellular uptake of nigella sativa oil-PLGA microparticle by PC-12 cell line

Similar Items