Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy

Objective: Quantification of medicinal plant, N. sativa oil, in biodegradable nanoparticles fabricated from PLGA and chitosan was impossible due to the difficulty in the oil extraction-partition method of which the nanoparticle did not dissolve in any solvent. Methods: FTIR method was developed t...

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Main Authors:	Doolaanea, Abd Almonem, Harun Ismail, Ahmad Fahmi, Mohamed, Farahidah
Format:	Article
Language:	English
Published:	Academic Sciences 2014
Subjects:	QD Chemistry RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/39644/ http://irep.iium.edu.my/39644/ http://irep.iium.edu.my/39644/1/Quantification_of_Nigella_Sativa_Oil_%28NSO%29_from_Biodegradable_PLGA_Nanoparticles_Using_FTIR_Spectroscopy.pdf

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spelling	iium-396442015-03-11T08:36:02Z http://irep.iium.edu.my/39644/ Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy Doolaanea, Abd Almonem Harun Ismail, Ahmad Fahmi Mohamed, Farahidah QD Chemistry RS Pharmacy and materia medica Objective: Quantification of medicinal plant, N. sativa oil, in biodegradable nanoparticles fabricated from PLGA and chitosan was impossible due to the difficulty in the oil extraction-partition method of which the nanoparticle did not dissolve in any solvent. Methods: FTIR method was developed to quantify the loading efficiency of NSO from the intact nanoparticles without the need to solubilise the nanoparticles and extract the NSO thereafter. Beer-Lambert law was applied in the quantification following selection of a few wave number combinations. Results: The method exhibited linearity in the range NSO/PLGA=5-150% with R2=0.9911, RSD=1.68%, LOD=0.89% and LOQ=2.68%. NSO-PLGA nanoparticles revealed complete encapsulation of NSO (loading efficiency=101.5±2.2%) while chitosan-NSO-PLGA nanoparticle showed lower loading efficiency (84.5±1.7%) due to presence of the hydrophilic polymer, i. e. chitosan. This method is fast and easy to apply and does not require sample processing. The method will help to accelerate and improve routine characterisation of NSO nanoparticles during development and optimisation stage. Academic Sciences 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/39644/1/Quantification_of_Nigella_Sativa_Oil_%28NSO%29_from_Biodegradable_PLGA_Nanoparticles_Using_FTIR_Spectroscopy.pdf Doolaanea, Abd Almonem and Harun Ismail, Ahmad Fahmi and Mohamed, Farahidah (2014) Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy. International Journal of Pharmacy and Pharmaceutical Sciences, 6 (10). pp. 228-232. ISSN 0975-1491 http://innovareacademics.in/journals/index.php/ijpps/article/view/2529
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry RS Pharmacy and materia medica
spellingShingle	QD Chemistry RS Pharmacy and materia medica Doolaanea, Abd Almonem Harun Ismail, Ahmad Fahmi Mohamed, Farahidah Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy
description	Objective: Quantification of medicinal plant, N. sativa oil, in biodegradable nanoparticles fabricated from PLGA and chitosan was impossible due to the difficulty in the oil extraction-partition method of which the nanoparticle did not dissolve in any solvent. Methods: FTIR method was developed to quantify the loading efficiency of NSO from the intact nanoparticles without the need to solubilise the nanoparticles and extract the NSO thereafter. Beer-Lambert law was applied in the quantification following selection of a few wave number combinations. Results: The method exhibited linearity in the range NSO/PLGA=5-150% with R2=0.9911, RSD=1.68%, LOD=0.89% and LOQ=2.68%. NSO-PLGA nanoparticles revealed complete encapsulation of NSO (loading efficiency=101.5±2.2%) while chitosan-NSO-PLGA nanoparticle showed lower loading efficiency (84.5±1.7%) due to presence of the hydrophilic polymer, i. e. chitosan. This method is fast and easy to apply and does not require sample processing. The method will help to accelerate and improve routine characterisation of NSO nanoparticles during development and optimisation stage.
format	Article
author	Doolaanea, Abd Almonem Harun Ismail, Ahmad Fahmi Mohamed, Farahidah
author_facet	Doolaanea, Abd Almonem Harun Ismail, Ahmad Fahmi Mohamed, Farahidah
author_sort	Doolaanea, Abd Almonem
title	Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy
title_short	Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy
title_full	Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy
title_fullStr	Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy
title_full_unstemmed	Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy
title_sort	quantification of nigella sativa oil (nso) from biodegradable plga nanoparticles using ftir spectroscopy
publisher	Academic Sciences
publishDate	2014
url	http://irep.iium.edu.my/39644/ http://irep.iium.edu.my/39644/ http://irep.iium.edu.my/39644/1/Quantification_of_Nigella_Sativa_Oil_%28NSO%29_from_Biodegradable_PLGA_Nanoparticles_Using_FTIR_Spectroscopy.pdf
first_indexed	2023-09-18T20:56:58Z
last_indexed	2023-09-18T20:56:58Z
_version_	1777410368972259328

Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy

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