Identification of aldolase C, actin related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens

Halal slaughtered chicken is a major concern amongst Muslims. Currently, stunning methods has been adopted worldwide aiming at maximizing production and fasten up the slaughtering process. However, stunning treatment which is not in compliance with the Islamic teaching will end up with unlawful chic...

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Main Authors:	Abu Samah, Norshahida, Amid, Azura, Yusof, Faridah
Format:	Conference or Workshop Item
Language:	English English
Published:	2014
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/39675/ http://irep.iium.edu.my/39675/ http://irep.iium.edu.my/39675/1/INHAC2014_Norshahida_10122014.pdf http://irep.iium.edu.my/39675/4/Programme_Tentative.pdf

id	iium-39675
recordtype	eprints
spelling	iium-396752018-05-24T05:23:00Z http://irep.iium.edu.my/39675/ Identification of aldolase C, actin related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens Abu Samah, Norshahida Amid, Azura Yusof, Faridah TP248.13 Biotechnology Halal slaughtered chicken is a major concern amongst Muslims. Currently, stunning methods has been adopted worldwide aiming at maximizing production and fasten up the slaughtering process. However, stunning treatment which is not in compliance with the Islamic teaching will end up with unlawful chickens for Muslim consumption. In this study, proteomics methods have been used to identify potential biomarkers for the effects of electrical stimulation on the protein profiles of chicken’s brain. The protein profiles were constructed in order to detect any differences in the protein expression and modifications. The electrical stimulation was conducted with different current and voltage. The different voltages studied were 10 V, 40 V and 70 V while the values for current studied were 0.25 A, 0.5 A, and 0.75 A. The profiles from these electrically stimulated chicken were compared to the 0 A, 0 V non stunned sample. After the proteomics analyses using 2D Platinum ImageMaster 6.0 and Matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry (MALDI-TOF) identification, Aldolase C, Actin Related Protein 2 Homolog (ARP2) and Enolase 1 were identified to be positively present in the brain sample of electrically stimulated samples. The over expression of the proteins were further confirmed at the transcriptional level by Real Time PCR. Results from MALDI-TOF and Real Time PCR were in agreement. Therefore, this identification of biomarkers for electrically stimulated chicken’s brain provides a novel approach on differentiating the stunned chickens from the non-stunned ones. 2014-11-17 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/39675/1/INHAC2014_Norshahida_10122014.pdf application/pdf en http://irep.iium.edu.my/39675/4/Programme_Tentative.pdf Abu Samah, Norshahida and Amid, Azura and Yusof, Faridah (2014) Identification of aldolase C, actin related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens. In: International Halal Conference 2014 (INHAC 2014) , 17th-18th November 2014, Istanbul, Turkey. (Unpublished) http://www.acis.uitm.edu.my/inhac2014
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Abu Samah, Norshahida Amid, Azura Yusof, Faridah Identification of aldolase C, actin related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens
description	Halal slaughtered chicken is a major concern amongst Muslims. Currently, stunning methods has been adopted worldwide aiming at maximizing production and fasten up the slaughtering process. However, stunning treatment which is not in compliance with the Islamic teaching will end up with unlawful chickens for Muslim consumption. In this study, proteomics methods have been used to identify potential biomarkers for the effects of electrical stimulation on the protein profiles of chicken’s brain. The protein profiles were constructed in order to detect any differences in the protein expression and modifications. The electrical stimulation was conducted with different current and voltage. The different voltages studied were 10 V, 40 V and 70 V while the values for current studied were 0.25 A, 0.5 A, and 0.75 A. The profiles from these electrically stimulated chicken were compared to the 0 A, 0 V non stunned sample. After the proteomics analyses using 2D Platinum ImageMaster 6.0 and Matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry (MALDI-TOF) identification, Aldolase C, Actin Related Protein 2 Homolog (ARP2) and Enolase 1 were identified to be positively present in the brain sample of electrically stimulated samples. The over expression of the proteins were further confirmed at the transcriptional level by Real Time PCR. Results from MALDI-TOF and Real Time PCR were in agreement. Therefore, this identification of biomarkers for electrically stimulated chicken’s brain provides a novel approach on differentiating the stunned chickens from the non-stunned ones.
format	Conference or Workshop Item
author	Abu Samah, Norshahida Amid, Azura Yusof, Faridah
author_facet	Abu Samah, Norshahida Amid, Azura Yusof, Faridah
author_sort	Abu Samah, Norshahida
title	Identification of aldolase C, actin related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens
title_short	Identification of aldolase C, actin related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens
title_full	Identification of aldolase C, actin related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens
title_fullStr	Identification of aldolase C, actin related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens
title_full_unstemmed	Identification of aldolase C, actin related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens
title_sort	identification of aldolase c, actin related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens
publishDate	2014
url	http://irep.iium.edu.my/39675/ http://irep.iium.edu.my/39675/ http://irep.iium.edu.my/39675/1/INHAC2014_Norshahida_10122014.pdf http://irep.iium.edu.my/39675/4/Programme_Tentative.pdf
first_indexed	2023-09-18T20:57:01Z
last_indexed	2023-09-18T20:57:01Z
_version_	1777410371885203456

Identification of aldolase C, actin related protein 2 homolog, and enolase 1 as potential biomarkers for electrically stunned chickens

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