Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres

Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres

In this study, model protein bovine serum albumin (BSA) loaded poly(lactide-co-glycolide) (PLGA) microspheres have been prepared by a conventional water-in-oil-in-water (w/o/w) and a modified water-in-oil-in-oil-in-water (w/o/o/w) double emulsion solvent evaporation method. The prepared microspheres...

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Main Authors: Rahman, Md. Mokhlesur, Mohamed, Awang, Haliza, Katas, Ansary , Rezaul H.
Format: Conference or Workshop Item
Language:English
Published: 2014
Subjects:
QH301 Biology
Online Access:http://irep.iium.edu.my/39998/
http://irep.iium.edu.my/39998/
http://irep.iium.edu.my/39998/1/39998.pdf
id iium-39998
recordtype eprints
spelling iium-399982018-06-11T03:48:38Z http://irep.iium.edu.my/39998/ Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres Rahman, Md. Mokhlesur Mohamed, Awang Haliza, Katas Ansary , Rezaul H. QH301 Biology In this study, model protein bovine serum albumin (BSA) loaded poly(lactide-co-glycolide) (PLGA) microspheres have been prepared by a conventional water-in-oil-in-water (w/o/w) and a modified water-in-oil-in-oil-in-water (w/o/o/w) double emulsion solvent evaporation method. The prepared microspheres were characterized with respect to their morphology, particle size, encapsulation efficiency, production yield, thermal properties and in vitro drug release. By using w/o/o/w method, a significant decrease in mean particle size and a significant increase in encapsulation efficiency were observed when a binary solvent mixture of ethyl acetate and dichloromethane was used. In w/o/o/w double emulsion solvent evaporation method, the optimized formulation of BSA loaded microspheres was nonporous, smooth-surfaced, and spherical shape under field-emission scanning electron microscope (FE-SEM) with a mean particle size of 3.95 µm and encapsulation efficiency of 98.46%. From 72 days In vitro release studies, microspheres prepared by modified (w/o/o/w) method with a combination of hydroxyl and carboxyl terminated PLGA polymers exhibited a significantly lower initial burst release followed by sustained and almost complete release compared to microspheres prepared by conventional w/o/w technique using a single PLGA polymer. It can be concluded that the modified w/o/o/w method can be proposed as a potential delivery system of therapeutic proteins. 2014-10-28 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/39998/1/39998.pdf Rahman, Md. Mokhlesur and Mohamed, Awang and Haliza, Katas and Ansary , Rezaul H. (2014) Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres. In: Malaysia International Biological Symposium 2014 (i-SIMBIOMAS 2014), 28th - 29th October 2014, Putrajaya. http://www.science.upm.edu.my/isimbiomas2014/
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QH301 Biology
spellingShingle QH301 Biology
Rahman, Md. Mokhlesur
Mohamed, Awang
Haliza, Katas
Ansary , Rezaul H.
Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres
description In this study, model protein bovine serum albumin (BSA) loaded poly(lactide-co-glycolide) (PLGA) microspheres have been prepared by a conventional water-in-oil-in-water (w/o/w) and a modified water-in-oil-in-oil-in-water (w/o/o/w) double emulsion solvent evaporation method. The prepared microspheres were characterized with respect to their morphology, particle size, encapsulation efficiency, production yield, thermal properties and in vitro drug release. By using w/o/o/w method, a significant decrease in mean particle size and a significant increase in encapsulation efficiency were observed when a binary solvent mixture of ethyl acetate and dichloromethane was used. In w/o/o/w double emulsion solvent evaporation method, the optimized formulation of BSA loaded microspheres was nonporous, smooth-surfaced, and spherical shape under field-emission scanning electron microscope (FE-SEM) with a mean particle size of 3.95 µm and encapsulation efficiency of 98.46%. From 72 days In vitro release studies, microspheres prepared by modified (w/o/o/w) method with a combination of hydroxyl and carboxyl terminated PLGA polymers exhibited a significantly lower initial burst release followed by sustained and almost complete release compared to microspheres prepared by conventional w/o/w technique using a single PLGA polymer. It can be concluded that the modified w/o/o/w method can be proposed as a potential delivery system of therapeutic proteins.
format Conference or Workshop Item
author Rahman, Md. Mokhlesur
Mohamed, Awang
Haliza, Katas
Ansary , Rezaul H.
author_facet Rahman, Md. Mokhlesur
Mohamed, Awang
Haliza, Katas
Ansary , Rezaul H.
author_sort Rahman, Md. Mokhlesur
title Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres
title_short Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres
title_full Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres
title_fullStr Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres
title_full_unstemmed Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres
title_sort preparation, characterization and reduction of burst release of bovine serum albumin (bsa) from biodegradable plga microspheres
publishDate 2014
url http://irep.iium.edu.my/39998/
http://irep.iium.edu.my/39998/
http://irep.iium.edu.my/39998/1/39998.pdf
first_indexed 2023-09-18T20:57:25Z
last_indexed 2023-09-18T20:57:25Z
_version_ 1777410396776300544

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