Comparative study on protein expression of chicken meat between Malaysian broilers and indigenous chicken

High consumer demand towards poultry meat has increased the production of broilers chicken.Due to high feed conversion ratio in broilers and administration of antibiotics in their feeds, broilers undergoes rapid juvenile growth in short time. The rapid growth has brought many health issues including...

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Main Authors:	Abu Hassan, Muhamad Shafiq, Muhamad Bunnori, Noraslinda
Format:	Conference or Workshop Item
Language:	English English
Published:	2014
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/40042/ http://irep.iium.edu.my/40042/ http://irep.iium.edu.my/40042/1/Malaysia_International_Biological_Symposium.pdf http://irep.iium.edu.my/40042/3/isimbiosis2014.pdf

id	iium-40042
recordtype	eprints
spelling	iium-400422018-06-19T07:11:58Z http://irep.iium.edu.my/40042/ Comparative study on protein expression of chicken meat between Malaysian broilers and indigenous chicken Abu Hassan, Muhamad Shafiq Muhamad Bunnori, Noraslinda TP248.13 Biotechnology High consumer demand towards poultry meat has increased the production of broilers chicken.Due to high feed conversion ratio in broilers and administration of antibiotics in their feeds, broilers undergoes rapid juvenile growth in short time. The rapid growth has brought many health issues including metabolic diseases and other defects that could alter the meat quality. Thus, proteomics approach will be done to compare the protein profile between broilers and indigenous chicken meat. Two types of meat sample from aforementioned chickens were homogenized with extraction buffer and quantified by using Bradford assay. Then, the protein samples were examined by using SDS-PAGE and further resolved by using 2D-PAGE. Highest protein expressed distributed between 40-50 kDa on SDS-PAGE and predicted to be actin and myosin. 2D-PAGE result showed that three protein spots were expressed higher in broilers compared to indigenous chicken. The candidates for this proteins are Serotransferrin (pI:6.7; mW:50-75 kDa), myosin binding protein (pI:5-6; mW: 50-75 kDa) and T-complex protein 1 subunit epsilon (pI:5-6; mW: 50-75 kDa). In conclusion, three proteins have been identified and it may contribute to the changes of meat quality in broilers. 2014-05-10 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/40042/1/Malaysia_International_Biological_Symposium.pdf application/pdf en http://irep.iium.edu.my/40042/3/isimbiosis2014.pdf Abu Hassan, Muhamad Shafiq and Muhamad Bunnori, Noraslinda (2014) Comparative study on protein expression of chicken meat between Malaysian broilers and indigenous chicken. In: Malaysia International Biological Symposium , 28th-29th October 2014, Putrajaya. (Unpublished) http://www.science.upm.edu.my/isimbiomas2014/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Abu Hassan, Muhamad Shafiq Muhamad Bunnori, Noraslinda Comparative study on protein expression of chicken meat between Malaysian broilers and indigenous chicken
description	High consumer demand towards poultry meat has increased the production of broilers chicken.Due to high feed conversion ratio in broilers and administration of antibiotics in their feeds, broilers undergoes rapid juvenile growth in short time. The rapid growth has brought many health issues including metabolic diseases and other defects that could alter the meat quality. Thus, proteomics approach will be done to compare the protein profile between broilers and indigenous chicken meat. Two types of meat sample from aforementioned chickens were homogenized with extraction buffer and quantified by using Bradford assay. Then, the protein samples were examined by using SDS-PAGE and further resolved by using 2D-PAGE. Highest protein expressed distributed between 40-50 kDa on SDS-PAGE and predicted to be actin and myosin. 2D-PAGE result showed that three protein spots were expressed higher in broilers compared to indigenous chicken. The candidates for this proteins are Serotransferrin (pI:6.7; mW:50-75 kDa), myosin binding protein (pI:5-6; mW: 50-75 kDa) and T-complex protein 1 subunit epsilon (pI:5-6; mW: 50-75 kDa). In conclusion, three proteins have been identified and it may contribute to the changes of meat quality in broilers.
format	Conference or Workshop Item
author	Abu Hassan, Muhamad Shafiq Muhamad Bunnori, Noraslinda
author_facet	Abu Hassan, Muhamad Shafiq Muhamad Bunnori, Noraslinda
author_sort	Abu Hassan, Muhamad Shafiq
title	Comparative study on protein expression of chicken meat between Malaysian broilers and indigenous chicken
title_short	Comparative study on protein expression of chicken meat between Malaysian broilers and indigenous chicken
title_full	Comparative study on protein expression of chicken meat between Malaysian broilers and indigenous chicken
title_fullStr	Comparative study on protein expression of chicken meat between Malaysian broilers and indigenous chicken
title_full_unstemmed	Comparative study on protein expression of chicken meat between Malaysian broilers and indigenous chicken
title_sort	comparative study on protein expression of chicken meat between malaysian broilers and indigenous chicken
publishDate	2014
url	http://irep.iium.edu.my/40042/ http://irep.iium.edu.my/40042/ http://irep.iium.edu.my/40042/1/Malaysia_International_Biological_Symposium.pdf http://irep.iium.edu.my/40042/3/isimbiosis2014.pdf
first_indexed	2023-09-18T20:57:29Z
last_indexed	2023-09-18T20:57:29Z
_version_	1777410401015693312

Comparative study on protein expression of chicken meat between Malaysian broilers and indigenous chicken

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