Phytochemical investigation of the leaves of tetracera scandens Linn and in vitro antidiabetic activity of hypoletin

Tetracera scandens Linn. (Dilleniaceae), locally known as Mempelas Kasar, is traditionally used in folk remedies by various indigenous people in different countries including Malaysia for the treatment of rheumatism, inflammatory diseases, hepatitis, internal pains, urinary disorders, dysentery, sor...

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Main Authors:	Ahmed, Qamar Uddin, Umar, Abdulrashid, Bakhtiar, M. Taher, Darnis, Deny Susanti, Mohamed Amiroudine, Mohamed Zaffar Ali, Latip, Jalifah
Other Authors:	Kasim, Azman
Format:	Conference or Workshop Item
Language:	English English
Published:	Springer 2014
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/40084/ http://irep.iium.edu.my/40084/ http://irep.iium.edu.my/40084/12/2014_ICSTSS_2.pdf http://irep.iium.edu.my/40084/6/mtb_bfm_chapter71.pdf

id	iium-40084
recordtype	eprints
spelling	iium-400842019-01-02T03:51:56Z http://irep.iium.edu.my/40084/ Phytochemical investigation of the leaves of tetracera scandens Linn and in vitro antidiabetic activity of hypoletin Ahmed, Qamar Uddin Umar, Abdulrashid Bakhtiar, M. Taher Darnis, Deny Susanti Mohamed Amiroudine, Mohamed Zaffar Ali Latip, Jalifah RS Pharmacy and materia medica Tetracera scandens Linn. (Dilleniaceae), locally known as Mempelas Kasar, is traditionally used in folk remedies by various indigenous people in different countries including Malaysia for the treatment of rheumatism, inflammatory diseases, hepatitis, internal pains, urinary disorders, dysentery, sore throat, gout and diabetes infirmities; for lowering hypertension; and for child birth. In this study, phytochemical analysis of T. scandens leaf methanol (MeOH) extract was carried out for the first time and afforded three terpenoids (stigmasterol, betulinic acid and an isomeric mixture of sitosterol (∆5) glycoside and stigmasterol (∆5,22) glycoside) and six flavonoids (namely, kaempferol, quercetin, isoscutellarein, hypoletin, astragalin and kaempferol-3-O-(6″-O-p-trans-coumaroyl) glucoside, a rare compound which has been isolated for the first time from family Dilleniaceae). Structures of all compounds were elucidated through extensive UV–Vis, mass, IR and NMR spectral analysis. The occurrence of all these compounds is being reported for the first time from this plant. Due to the rare occurrence of hypoletin (3′,4′,5,7,8-pentahydroxyflavone) in plants, its in vitro antidiabetic effect was explored. Hence, the treatment of hypoletin in a dose-dependent manner was examined on the induction of lipid accumulation by using Oil Red O staining and glucose regulation in 3T3-L1 adipocytes in vitro with regard to its preventive role in the management of diabetes. After 8 days, morphological changes and high lipid accumulation activity were observed in cells treated with 10 μg/mL of hypoletin concentration (P < 0.01). In addition, the intracellular fat accumulation increased by up to 79.7 % relative to MDI-treated control cells at a dose of 10 μg/mL. Furthermore, insulin-induced 2-deoxy-d-[3H] glucose uptake was significantly increased (P < 0.001) in hypoletin-treated cells as compared to control (DMSO cells). Our results suggest that hypoletin could be useful for the management of type 2 diabetes due to its adipocyte stimulation and glucose uptake activity. Springer Kasim, Azman Wan Omar, Wan Siti Atikah Abdul Razak, Nor Hidayatun Musa, Nor Lailatul Wahidah Ab Halim, Roslilee Mohamed, Siti Rosiah 2014 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/40084/12/2014_ICSTSS_2.pdf application/pdf en http://irep.iium.edu.my/40084/6/mtb_bfm_chapter71.pdf Ahmed, Qamar Uddin and Umar, Abdulrashid and Bakhtiar, M. Taher and Darnis, Deny Susanti and Mohamed Amiroudine, Mohamed Zaffar Ali and Latip, Jalifah (2014) Phytochemical investigation of the leaves of tetracera scandens Linn and in vitro antidiabetic activity of hypoletin. In: International Conference on Science, Technology and Social Sciences (ICSTSS) 2012, 20-22 Nov 2012, Kuantan, Pahang. http://www.springer.com/social+sciences/wellbeing+%26+quality-of-life/book/978-981-287-076-6
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Ahmed, Qamar Uddin Umar, Abdulrashid Bakhtiar, M. Taher Darnis, Deny Susanti Mohamed Amiroudine, Mohamed Zaffar Ali Latip, Jalifah Phytochemical investigation of the leaves of tetracera scandens Linn and in vitro antidiabetic activity of hypoletin
description	Tetracera scandens Linn. (Dilleniaceae), locally known as Mempelas Kasar, is traditionally used in folk remedies by various indigenous people in different countries including Malaysia for the treatment of rheumatism, inflammatory diseases, hepatitis, internal pains, urinary disorders, dysentery, sore throat, gout and diabetes infirmities; for lowering hypertension; and for child birth. In this study, phytochemical analysis of T. scandens leaf methanol (MeOH) extract was carried out for the first time and afforded three terpenoids (stigmasterol, betulinic acid and an isomeric mixture of sitosterol (∆5) glycoside and stigmasterol (∆5,22) glycoside) and six flavonoids (namely, kaempferol, quercetin, isoscutellarein, hypoletin, astragalin and kaempferol-3-O-(6″-O-p-trans-coumaroyl) glucoside, a rare compound which has been isolated for the first time from family Dilleniaceae). Structures of all compounds were elucidated through extensive UV–Vis, mass, IR and NMR spectral analysis. The occurrence of all these compounds is being reported for the first time from this plant. Due to the rare occurrence of hypoletin (3′,4′,5,7,8-pentahydroxyflavone) in plants, its in vitro antidiabetic effect was explored. Hence, the treatment of hypoletin in a dose-dependent manner was examined on the induction of lipid accumulation by using Oil Red O staining and glucose regulation in 3T3-L1 adipocytes in vitro with regard to its preventive role in the management of diabetes. After 8 days, morphological changes and high lipid accumulation activity were observed in cells treated with 10 μg/mL of hypoletin concentration (P < 0.01). In addition, the intracellular fat accumulation increased by up to 79.7 % relative to MDI-treated control cells at a dose of 10 μg/mL. Furthermore, insulin-induced 2-deoxy-d-[3H] glucose uptake was significantly increased (P < 0.001) in hypoletin-treated cells as compared to control (DMSO cells). Our results suggest that hypoletin could be useful for the management of type 2 diabetes due to its adipocyte stimulation and glucose uptake activity.
author2	Kasim, Azman
author_facet	Kasim, Azman Ahmed, Qamar Uddin Umar, Abdulrashid Bakhtiar, M. Taher Darnis, Deny Susanti Mohamed Amiroudine, Mohamed Zaffar Ali Latip, Jalifah
format	Conference or Workshop Item
author	Ahmed, Qamar Uddin Umar, Abdulrashid Bakhtiar, M. Taher Darnis, Deny Susanti Mohamed Amiroudine, Mohamed Zaffar Ali Latip, Jalifah
author_sort	Ahmed, Qamar Uddin
title	Phytochemical investigation of the leaves of tetracera scandens Linn and in vitro antidiabetic activity of hypoletin
title_short	Phytochemical investigation of the leaves of tetracera scandens Linn and in vitro antidiabetic activity of hypoletin
title_full	Phytochemical investigation of the leaves of tetracera scandens Linn and in vitro antidiabetic activity of hypoletin
title_fullStr	Phytochemical investigation of the leaves of tetracera scandens Linn and in vitro antidiabetic activity of hypoletin
title_full_unstemmed	Phytochemical investigation of the leaves of tetracera scandens Linn and in vitro antidiabetic activity of hypoletin
title_sort	phytochemical investigation of the leaves of tetracera scandens linn and in vitro antidiabetic activity of hypoletin
publisher	Springer
publishDate	2014
url	http://irep.iium.edu.my/40084/ http://irep.iium.edu.my/40084/ http://irep.iium.edu.my/40084/12/2014_ICSTSS_2.pdf http://irep.iium.edu.my/40084/6/mtb_bfm_chapter71.pdf
first_indexed	2023-09-18T20:57:31Z
last_indexed	2023-09-18T20:57:31Z
_version_	1777410403485089792

Phytochemical investigation of the leaves of tetracera scandens Linn and in vitro antidiabetic activity of hypoletin

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