High performance liquid chromatographic determination of mefenamic acid in human plasma using UV vis detector

Objective: Mefenamic Acid (MA) is a non-steroidal anti-inflammatory drug (NSAIDs). This drug provides analgesic and antipyretic (fever reducing effect) and higher doses, anti-inflammatory effect. This study is focused to develop a rapid and sensitive method for the detection of mefenamic acid in hum...

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Main Authors:	Helaluddin, Abul Bashar Mohammed, Mohamad, Huda Jamilah, Alaama, Mohammed, Amiruddin, Noor Syafawati
Format:	Article
Language:	English English
Published:	Academic Sciences 2014
Subjects:	QD Chemistry RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/40411/ http://irep.iium.edu.my/40411/ http://irep.iium.edu.my/40411/1/HIGH_PERFORMANCE_LIQUID_CHROMATOGRAPHIC_DETERMINATION_OF_MEFENAMIC_ACID_IN_HUMAN_PLASMA_USING_UV_VIS_DETECTOR.pdf http://irep.iium.edu.my/40411/4/40411_High%20performance%20liquid%20chromatographic.SCOPUSpdf.pdf

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spelling	iium-404112018-05-24T02:43:35Z http://irep.iium.edu.my/40411/ High performance liquid chromatographic determination of mefenamic acid in human plasma using UV vis detector Helaluddin, Abul Bashar Mohammed Mohamad, Huda Jamilah Alaama, Mohammed Amiruddin, Noor Syafawati QD Chemistry RS Pharmacy and materia medica Objective: Mefenamic Acid (MA) is a non-steroidal anti-inflammatory drug (NSAIDs). This drug provides analgesic and antipyretic (fever reducing effect) and higher doses, anti-inflammatory effect. This study is focused to develop a rapid and sensitive method for the detection of mefenamic acid in human plasma. Methods: Protein precipitation technique using acetonitrile was used. Chromatographic separation was achieved on Agilent Zorbax Eclipse XDB-C18 Results: The recovery was found 83% for MA. The method was validated according to the Centre for Drug Evaluation and Research (CDER) guidelines. Calibration plot was linear within the range from 250 to 5000ng ml(150 mm x 4.6 mm, i. d 3.5 μm) with a mobile phase consisting of acetonitrile and 2% triethylamine (pH was adjusted to 4.2 with phosphoric acid) in a ratio of 60:40. The retention time for mefenamic acid and diclofenac was 5.4 and 3.9 minutes respectively. The mefenamic acid was monitored at 280 nm using variable-wavelength detector. -1 with the coefficient of determination (r2 Conclusion: The results were in compliance with CDER guideline. ) of ≥ 0.99. The quality control samples of mefenamic acid which was termed as low (L), medium (M) and high (H) were analysed to get the precision and accuracy. The accuracy for intra-day for L, M and H was 99.71%, 93.8% and 89.52% while for inter day were 97.67%, 93.46% and 91.67% respectively. On the other hand, coefficient variance (CV) for intra-day precision for L, M and H was found 2.57%, 2.45% and 1.45% and for inter day CV were 3.11%, 5.5% and 4.37% respectively. Diclofenac sodium was used as internal standard for this study. Keywords: HPLC, Mefenamic Acid, Human plasma, UV detector, Protein precipitation. Academic Sciences 2014-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/40411/1/HIGH_PERFORMANCE_LIQUID_CHROMATOGRAPHIC_DETERMINATION_OF_MEFENAMIC_ACID_IN_HUMAN_PLASMA_USING_UV_VIS_DETECTOR.pdf application/pdf en http://irep.iium.edu.my/40411/4/40411_High%20performance%20liquid%20chromatographic.SCOPUSpdf.pdf Helaluddin, Abul Bashar Mohammed and Mohamad, Huda Jamilah and Alaama, Mohammed and Amiruddin, Noor Syafawati (2014) High performance liquid chromatographic determination of mefenamic acid in human plasma using UV vis detector. International Journal of Pharmacy and Pharmaceutical Sciences, 6 (11). pp. 167-170. ISSN 0975-1491 http://innovareacademics.in/journals/index.php/ijpps/article/view/2954
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry RS Pharmacy and materia medica
spellingShingle	QD Chemistry RS Pharmacy and materia medica Helaluddin, Abul Bashar Mohammed Mohamad, Huda Jamilah Alaama, Mohammed Amiruddin, Noor Syafawati High performance liquid chromatographic determination of mefenamic acid in human plasma using UV vis detector
description	Objective: Mefenamic Acid (MA) is a non-steroidal anti-inflammatory drug (NSAIDs). This drug provides analgesic and antipyretic (fever reducing effect) and higher doses, anti-inflammatory effect. This study is focused to develop a rapid and sensitive method for the detection of mefenamic acid in human plasma. Methods: Protein precipitation technique using acetonitrile was used. Chromatographic separation was achieved on Agilent Zorbax Eclipse XDB-C18 Results: The recovery was found 83% for MA. The method was validated according to the Centre for Drug Evaluation and Research (CDER) guidelines. Calibration plot was linear within the range from 250 to 5000ng ml(150 mm x 4.6 mm, i. d 3.5 μm) with a mobile phase consisting of acetonitrile and 2% triethylamine (pH was adjusted to 4.2 with phosphoric acid) in a ratio of 60:40. The retention time for mefenamic acid and diclofenac was 5.4 and 3.9 minutes respectively. The mefenamic acid was monitored at 280 nm using variable-wavelength detector. -1 with the coefficient of determination (r2 Conclusion: The results were in compliance with CDER guideline. ) of ≥ 0.99. The quality control samples of mefenamic acid which was termed as low (L), medium (M) and high (H) were analysed to get the precision and accuracy. The accuracy for intra-day for L, M and H was 99.71%, 93.8% and 89.52% while for inter day were 97.67%, 93.46% and 91.67% respectively. On the other hand, coefficient variance (CV) for intra-day precision for L, M and H was found 2.57%, 2.45% and 1.45% and for inter day CV were 3.11%, 5.5% and 4.37% respectively. Diclofenac sodium was used as internal standard for this study. Keywords: HPLC, Mefenamic Acid, Human plasma, UV detector, Protein precipitation.
format	Article
author	Helaluddin, Abul Bashar Mohammed Mohamad, Huda Jamilah Alaama, Mohammed Amiruddin, Noor Syafawati
author_facet	Helaluddin, Abul Bashar Mohammed Mohamad, Huda Jamilah Alaama, Mohammed Amiruddin, Noor Syafawati
author_sort	Helaluddin, Abul Bashar Mohammed
title	High performance liquid chromatographic determination of mefenamic acid in human plasma using UV vis detector
title_short	High performance liquid chromatographic determination of mefenamic acid in human plasma using UV vis detector
title_full	High performance liquid chromatographic determination of mefenamic acid in human plasma using UV vis detector
title_fullStr	High performance liquid chromatographic determination of mefenamic acid in human plasma using UV vis detector
title_full_unstemmed	High performance liquid chromatographic determination of mefenamic acid in human plasma using UV vis detector
title_sort	high performance liquid chromatographic determination of mefenamic acid in human plasma using uv vis detector
publisher	Academic Sciences
publishDate	2014
url	http://irep.iium.edu.my/40411/ http://irep.iium.edu.my/40411/ http://irep.iium.edu.my/40411/1/HIGH_PERFORMANCE_LIQUID_CHROMATOGRAPHIC_DETERMINATION_OF_MEFENAMIC_ACID_IN_HUMAN_PLASMA_USING_UV_VIS_DETECTOR.pdf http://irep.iium.edu.my/40411/4/40411_High%20performance%20liquid%20chromatographic.SCOPUSpdf.pdf
first_indexed	2023-09-18T20:57:59Z
last_indexed	2023-09-18T20:57:59Z
_version_	1777410432421593088

High performance liquid chromatographic determination of mefenamic acid in human plasma using UV vis detector

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