In vivo toxicity study of aqueous extracts of cymbopogon citratus (Lemongrass)

Aqueous extraction was done by carefully selecting, washing, chopping and blending of 2 kg of C. citratus in 1 L of distilled water. The mixture was then simmered in a 50 °C water bath for an hour, finely filtered, frozen in a -80 °C freezer and then freeze dried. The 20 mice used were divided into...

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Main Authors:	Maetalong, Majid, Darnis, Deny Susanti, Zamli, Zaitunnatakhin, Ramli, Norazsida
Format:	Conference or Workshop Item
Language:	English English
Published:	2014
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/40512/ http://irep.iium.edu.my/40512/2/Poster_sympo_3.pdf http://irep.iium.edu.my/40512/5/lemongrass.pdf

id	iium-40512
recordtype	eprints
spelling	iium-405122017-06-15T05:59:13Z http://irep.iium.edu.my/40512/ In vivo toxicity study of aqueous extracts of cymbopogon citratus (Lemongrass) Maetalong, Majid Darnis, Deny Susanti Zamli, Zaitunnatakhin Ramli, Norazsida Q Science (General) Aqueous extraction was done by carefully selecting, washing, chopping and blending of 2 kg of C. citratus in 1 L of distilled water. The mixture was then simmered in a 50 °C water bath for an hour, finely filtered, frozen in a -80 °C freezer and then freeze dried. The 20 mice used were divided into two groups, control and experimental groups with equal sex ratio in each group. The experimental mice were orally administered with a single dose of 5000 mg/kg of C. citratus extracts. Behavioral observation was done for 14 days then all mice were sacrificed. Body weight measurements were taken before and after the 14 days, as well the weights of livers, kidneys and spleens of all mice were also taken after sacrifice. Histopathology examination was performed on all collected organs. Behavioural observation showed in the first hour an initial reduction in the locomotor activity, tachypnea, perioral and general tremor and piloerection. By the 3rd hour, emaciation was noticed, but with less stress and few sings of diarrhoea. The 4th hour showed signs of improvement and increased activity. The subsequent daily observation for 14 days showed no apparent signs of toxicity with a return of normal behaviour. Body weight comparison showed a slight increase in the mean weight of experimental mice, with no significance in the organ weight variation, or the sex as factor. Histopathology sections of the experimental mice have showed mild signs of toxicity, like pyknotic cells, colour variation due to inflammation, swelling and disrupted cells architecture. 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/40512/2/Poster_sympo_3.pdf application/pdf en http://irep.iium.edu.my/40512/5/lemongrass.pdf Maetalong, Majid and Darnis, Deny Susanti and Zamli, Zaitunnatakhin and Ramli, Norazsida (2014) In vivo toxicity study of aqueous extracts of cymbopogon citratus (Lemongrass). In: 5th Malaysian Symposium of Biomedical Science, 10th-11th May 2014, UKM Kuala Lumpur Campus, Kuala Lumpur.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Maetalong, Majid Darnis, Deny Susanti Zamli, Zaitunnatakhin Ramli, Norazsida In vivo toxicity study of aqueous extracts of cymbopogon citratus (Lemongrass)
description	Aqueous extraction was done by carefully selecting, washing, chopping and blending of 2 kg of C. citratus in 1 L of distilled water. The mixture was then simmered in a 50 °C water bath for an hour, finely filtered, frozen in a -80 °C freezer and then freeze dried. The 20 mice used were divided into two groups, control and experimental groups with equal sex ratio in each group. The experimental mice were orally administered with a single dose of 5000 mg/kg of C. citratus extracts. Behavioral observation was done for 14 days then all mice were sacrificed. Body weight measurements were taken before and after the 14 days, as well the weights of livers, kidneys and spleens of all mice were also taken after sacrifice. Histopathology examination was performed on all collected organs. Behavioural observation showed in the first hour an initial reduction in the locomotor activity, tachypnea, perioral and general tremor and piloerection. By the 3rd hour, emaciation was noticed, but with less stress and few sings of diarrhoea. The 4th hour showed signs of improvement and increased activity. The subsequent daily observation for 14 days showed no apparent signs of toxicity with a return of normal behaviour. Body weight comparison showed a slight increase in the mean weight of experimental mice, with no significance in the organ weight variation, or the sex as factor. Histopathology sections of the experimental mice have showed mild signs of toxicity, like pyknotic cells, colour variation due to inflammation, swelling and disrupted cells architecture.
format	Conference or Workshop Item
author	Maetalong, Majid Darnis, Deny Susanti Zamli, Zaitunnatakhin Ramli, Norazsida
author_facet	Maetalong, Majid Darnis, Deny Susanti Zamli, Zaitunnatakhin Ramli, Norazsida
author_sort	Maetalong, Majid
title	In vivo toxicity study of aqueous extracts of cymbopogon citratus (Lemongrass)
title_short	In vivo toxicity study of aqueous extracts of cymbopogon citratus (Lemongrass)
title_full	In vivo toxicity study of aqueous extracts of cymbopogon citratus (Lemongrass)
title_fullStr	In vivo toxicity study of aqueous extracts of cymbopogon citratus (Lemongrass)
title_full_unstemmed	In vivo toxicity study of aqueous extracts of cymbopogon citratus (Lemongrass)
title_sort	in vivo toxicity study of aqueous extracts of cymbopogon citratus (lemongrass)
publishDate	2014
url	http://irep.iium.edu.my/40512/ http://irep.iium.edu.my/40512/2/Poster_sympo_3.pdf http://irep.iium.edu.my/40512/5/lemongrass.pdf
first_indexed	2023-09-18T20:58:09Z
last_indexed	2023-09-18T20:58:09Z
_version_	1777410443521818624

In vivo toxicity study of aqueous extracts of cymbopogon citratus (Lemongrass)

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