Molecular identification and characterization of a bacterium that has potential to degrade low concentration of haloalkanoic acid

Most halogenated compounds are major environmental pollutants. Pollution from industrial chemicals and herbicide/pesticide from agricultural activities cause problem to the society. The main objectives were to isolate and characterize the bacterium that can grow in halogenated substrate as the sole...

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Main Authors:	Amini, S, Zulkifly, Ahmad Hafiz, Wong, Wen-Yong, Huyop, Fahrul
Format:	Article
Language:	English
Published:	Academic Journals Inc. 2011
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/40655/ http://irep.iium.edu.my/40655/ http://irep.iium.edu.my/40655/ http://irep.iium.edu.my/40655/1/Molecular_identification_and_characterization_of_a_bacterium_that_has_potential_to_degrade_low_concentration_of_haloalkanoic_acid.pdf

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spelling	iium-406552015-01-14T02:16:25Z http://irep.iium.edu.my/40655/ Molecular identification and characterization of a bacterium that has potential to degrade low concentration of haloalkanoic acid Amini, S Zulkifly, Ahmad Hafiz Wong, Wen-Yong Huyop, Fahrul Q Science (General) Most halogenated compounds are major environmental pollutants. Pollution from industrial chemicals and herbicide/pesticide from agricultural activities cause problem to the society. The main objectives were to isolate and characterize the bacterium that can grow in halogenated substrate as the sole source of carbon. A common microbiological and molecular techniques were adapted for further characterization. In current study, a soil microorganism, identified as Aminobacter sp. SA1 by partial biochemical and 16S rRNA sequencing method was isolated with 2,2DCP (2,2-dichloropropionic acid) as the sole carbon and energy source. This bacterium has the ability to degrade 2,2DCP (2,2-dichloropropionic acid) and D,L2CP (D,L-2-chloropropionic acid) at 20 mM with cells doubling time of 7 h in 2,2DCP medium. The bacterium was able to grow at low substrate concentration of 1 mM 2,2DCP (20 times lower than normal growth concentration) with cells doubling time of approximately 7 h suggesting the dehalogenase enzyme(s) that present in the bacterium has high affinity towards the substrate. However, at 0.1 mM 2,2DCP (200 times lower than normal concentration) no halide ions were detected in the growth medium suggesting no growth was observed. The investigation highlighted degradation at low substrate concentration suggested that the isolated bacterium can live in low level of halogenated compounds. This observation may or may not involve new kinds of dehalogenases. The presence of more than one dehalogenases in a bacterial system is far from clear Academic Journals Inc. 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/40655/1/Molecular_identification_and_characterization_of_a_bacterium_that_has_potential_to_degrade_low_concentration_of_haloalkanoic_acid.pdf Amini, S and Zulkifly, Ahmad Hafiz and Wong, Wen-Yong and Huyop, Fahrul (2011) Molecular identification and characterization of a bacterium that has potential to degrade low concentration of haloalkanoic acid. Research Journal of Microbiology, 6 (6). pp. 552-559. ISSN 1816-4935 http://docsdrive.com/pdfs/academicjournals/jm/2011/552-559.pdf 10.3923/jm.2011.552.559
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Amini, S Zulkifly, Ahmad Hafiz Wong, Wen-Yong Huyop, Fahrul Molecular identification and characterization of a bacterium that has potential to degrade low concentration of haloalkanoic acid
description	Most halogenated compounds are major environmental pollutants. Pollution from industrial chemicals and herbicide/pesticide from agricultural activities cause problem to the society. The main objectives were to isolate and characterize the bacterium that can grow in halogenated substrate as the sole source of carbon. A common microbiological and molecular techniques were adapted for further characterization. In current study, a soil microorganism, identified as Aminobacter sp. SA1 by partial biochemical and 16S rRNA sequencing method was isolated with 2,2DCP (2,2-dichloropropionic acid) as the sole carbon and energy source. This bacterium has the ability to degrade 2,2DCP (2,2-dichloropropionic acid) and D,L2CP (D,L-2-chloropropionic acid) at 20 mM with cells doubling time of 7 h in 2,2DCP medium. The bacterium was able to grow at low substrate concentration of 1 mM 2,2DCP (20 times lower than normal growth concentration) with cells doubling time of approximately 7 h suggesting the dehalogenase enzyme(s) that present in the bacterium has high affinity towards the substrate. However, at 0.1 mM 2,2DCP (200 times lower than normal concentration) no halide ions were detected in the growth medium suggesting no growth was observed. The investigation highlighted degradation at low substrate concentration suggested that the isolated bacterium can live in low level of halogenated compounds. This observation may or may not involve new kinds of dehalogenases. The presence of more than one dehalogenases in a bacterial system is far from clear
format	Article
author	Amini, S Zulkifly, Ahmad Hafiz Wong, Wen-Yong Huyop, Fahrul
author_facet	Amini, S Zulkifly, Ahmad Hafiz Wong, Wen-Yong Huyop, Fahrul
author_sort	Amini, S
title	Molecular identification and characterization of a bacterium that has potential to degrade low concentration of haloalkanoic acid
title_short	Molecular identification and characterization of a bacterium that has potential to degrade low concentration of haloalkanoic acid
title_full	Molecular identification and characterization of a bacterium that has potential to degrade low concentration of haloalkanoic acid
title_fullStr	Molecular identification and characterization of a bacterium that has potential to degrade low concentration of haloalkanoic acid
title_full_unstemmed	Molecular identification and characterization of a bacterium that has potential to degrade low concentration of haloalkanoic acid
title_sort	molecular identification and characterization of a bacterium that has potential to degrade low concentration of haloalkanoic acid
publisher	Academic Journals Inc.
publishDate	2011
url	http://irep.iium.edu.my/40655/ http://irep.iium.edu.my/40655/ http://irep.iium.edu.my/40655/ http://irep.iium.edu.my/40655/1/Molecular_identification_and_characterization_of_a_bacterium_that_has_potential_to_degrade_low_concentration_of_haloalkanoic_acid.pdf
first_indexed	2023-09-18T20:58:20Z
last_indexed	2023-09-18T20:58:20Z
_version_	1777410454378774528

Molecular identification and characterization of a bacterium that has potential to degrade low concentration of haloalkanoic acid

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