Antidiabetic activity of the leaves of tetracera indica merr. (Dilleniaceae) in vivo and in vitro

In folk remedies, the leaves of Tetracera indicaare used to treat diabetes in Malaysia. This study was aimed at investigating the antidiabetic potential of T. indica leaves in vivo and in vitro to prove its usefulness in diabetes. Aqueous (AQ) and methanol (MEOH) extracts of T. indica leaves were...

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Main Authors:	Ahmed, Qamar Uddin, Taher, Muhammad
Format:	Article
Language:	English
Published:	Academic Journals 2012
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/40983/ http://irep.iium.edu.my/40983/ http://irep.iium.edu.my/40983/ http://irep.iium.edu.my/40983/1/Antidiabetic_activity_of_the_leaves_of_Tetracera_indica.pdf

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spelling	iium-409832015-03-05T08:20:51Z http://irep.iium.edu.my/40983/ Antidiabetic activity of the leaves of tetracera indica merr. (Dilleniaceae) in vivo and in vitro Ahmed, Qamar Uddin Taher, Muhammad RS Pharmacy and materia medica In folk remedies, the leaves of Tetracera indicaare used to treat diabetes in Malaysia. This study was aimed at investigating the antidiabetic potential of T. indica leaves in vivo and in vitro to prove its usefulness in diabetes. Aqueous (AQ) and methanol (MEOH) extracts of T. indica leaves were administered to normal and alloxan induced diabetic male albino rats (Sprague Dawley strain). Two doses of each extract (250 and 500 mg/kg B.W) were evaluated for antidiabetic activity in vivo. The blood glucose levels were measured at 0, 2, 4, 6 and 8 h after oral administration of extracts. Comparison was made with glibenclamide (GLBC). For the in vitro method, the effect of both extracts on the lipid accumulation of 3T3-L1 adipocytes was analyzed by using Oil Red O staining. In addition, 2- deoxy-D-[3H] was used to measure the effect of the extracts on glucose uptake activities. Both extracts exhibited antihyperglycemic activity in alloxan induced diabetic rats, however in normal rats no hypoglycemic activity was observed, when compared with both +ve and -ve controlled groups. The LD50 of both extracts was found to be more than 5000 mg/kg body weight and no lethal toxicity was observed within this range. For in vitro analysis, AQ extract was found to reduce triglyceride accumulation on 3T3-L1 cells in a dose-dependent manner whereas cells treated with MEOH extract significantly induced lipid accumulation. Besides, 2-deoxy-D-[3H] glucose uptake activities were significantly different at dose 200 μg/mL (P < 0.05 compared to DMSO control). Moreover, the data were significant (P > 0.05) with MDI (inducer) and metformin which were used as the positive control for the in vitro assay. Academic Journals 2012-12-25 Article PeerReviewed application/pdf en http://irep.iium.edu.my/40983/1/Antidiabetic_activity_of_the_leaves_of_Tetracera_indica.pdf Ahmed, Qamar Uddin and Taher, Muhammad (2012) Antidiabetic activity of the leaves of tetracera indica merr. (Dilleniaceae) in vivo and in vitro. Journal of Medicinal Plants Research, 6 (49). pp. 5912-5922. ISSN 1996-0875 http://www.academicjournals.org/JMPR DOI: 10.5897/JMPR12.443
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Ahmed, Qamar Uddin Taher, Muhammad Antidiabetic activity of the leaves of tetracera indica merr. (Dilleniaceae) in vivo and in vitro
description	In folk remedies, the leaves of Tetracera indicaare used to treat diabetes in Malaysia. This study was aimed at investigating the antidiabetic potential of T. indica leaves in vivo and in vitro to prove its usefulness in diabetes. Aqueous (AQ) and methanol (MEOH) extracts of T. indica leaves were administered to normal and alloxan induced diabetic male albino rats (Sprague Dawley strain). Two doses of each extract (250 and 500 mg/kg B.W) were evaluated for antidiabetic activity in vivo. The blood glucose levels were measured at 0, 2, 4, 6 and 8 h after oral administration of extracts. Comparison was made with glibenclamide (GLBC). For the in vitro method, the effect of both extracts on the lipid accumulation of 3T3-L1 adipocytes was analyzed by using Oil Red O staining. In addition, 2- deoxy-D-[3H] was used to measure the effect of the extracts on glucose uptake activities. Both extracts exhibited antihyperglycemic activity in alloxan induced diabetic rats, however in normal rats no hypoglycemic activity was observed, when compared with both +ve and -ve controlled groups. The LD50 of both extracts was found to be more than 5000 mg/kg body weight and no lethal toxicity was observed within this range. For in vitro analysis, AQ extract was found to reduce triglyceride accumulation on 3T3-L1 cells in a dose-dependent manner whereas cells treated with MEOH extract significantly induced lipid accumulation. Besides, 2-deoxy-D-[3H] glucose uptake activities were significantly different at dose 200 μg/mL (P < 0.05 compared to DMSO control). Moreover, the data were significant (P > 0.05) with MDI (inducer) and metformin which were used as the positive control for the in vitro assay.
format	Article
author	Ahmed, Qamar Uddin Taher, Muhammad
author_facet	Ahmed, Qamar Uddin Taher, Muhammad
author_sort	Ahmed, Qamar Uddin
title	Antidiabetic activity of the leaves of tetracera indica merr. (Dilleniaceae) in vivo and in vitro
title_short	Antidiabetic activity of the leaves of tetracera indica merr. (Dilleniaceae) in vivo and in vitro
title_full	Antidiabetic activity of the leaves of tetracera indica merr. (Dilleniaceae) in vivo and in vitro
title_fullStr	Antidiabetic activity of the leaves of tetracera indica merr. (Dilleniaceae) in vivo and in vitro
title_full_unstemmed	Antidiabetic activity of the leaves of tetracera indica merr. (Dilleniaceae) in vivo and in vitro
title_sort	antidiabetic activity of the leaves of tetracera indica merr. (dilleniaceae) in vivo and in vitro
publisher	Academic Journals
publishDate	2012
url	http://irep.iium.edu.my/40983/ http://irep.iium.edu.my/40983/ http://irep.iium.edu.my/40983/ http://irep.iium.edu.my/40983/1/Antidiabetic_activity_of_the_leaves_of_Tetracera_indica.pdf
first_indexed	2023-09-18T20:58:42Z
last_indexed	2023-09-18T20:58:42Z
_version_	1777410478269530112

Antidiabetic activity of the leaves of tetracera indica merr. (Dilleniaceae) in vivo and in vitro

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