Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis

The effectiveness of poultry stunning in producing swift slaughtering was analysed in response to the time needed for the chickens to become insensible upon neck cutting (Td) and the induction of myofiber apoptosis. In total, 49 chicken broilers (BW of 2.17 ± .24 kg) were sacrificed with pre-slaught...

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Main Authors:	Shahdan, Intan Azura, Rahman, Mohammad Tariqur
Format:	Article
Language:	English English
Published:	Universiti Putra Malaysia 2014
Subjects:	S Agriculture (General)
Online Access:	http://irep.iium.edu.my/41256/ http://irep.iium.edu.my/41256/ http://irep.iium.edu.my/41256/1/31_IFRJ_21_%2806%29_2014_Intan_083.pdf http://irep.iium.edu.my/41256/6/41256_Effect%20of%20pre-slaughter_scopus.pdf

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recordtype	eprints
spelling	iium-412562017-09-19T04:04:52Z http://irep.iium.edu.my/41256/ Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis Shahdan, Intan Azura Rahman, Mohammad Tariqur S Agriculture (General) The effectiveness of poultry stunning in producing swift slaughtering was analysed in response to the time needed for the chickens to become insensible upon neck cutting (Td) and the induction of myofiber apoptosis. In total, 49 chicken broilers (BW of 2.17 ± .24 kg) were sacrificed with pre-slaughter stunning, using a constant voltage stunner where the electric current varied between 7.2 to 124.3 mA, and without stunning. The electric current applied during stunning was found to have no effect on Td. Number of apoptotic myonuclei did not vary among stunned and unstunned meat. Apoptosis inducing factor (AIF) and caspase 3 expressions were also not detected in the meat samples of both stunned and unstunned groups at 1 d postmortem. Since the slaughtering process and stunning are associated with stress, the expression of 70 kDa-heat shock protein (Hsp70) was investigated. Moreover Hsp70 is also an inhibitor of apoptosis, by preventing the activation of AIF and apoptosome which stimulates caspase 3 activation. However, expression of Hsp70 was not induced in both stunned groups and unstunned groups. Together, this study found that poultry stunning does not affect Td and myofiber apoptosis. Universiti Putra Malaysia 2014-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/41256/1/31_IFRJ_21_%2806%29_2014_Intan_083.pdf application/pdf en http://irep.iium.edu.my/41256/6/41256_Effect%20of%20pre-slaughter_scopus.pdf Shahdan, Intan Azura and Rahman, Mohammad Tariqur (2014) Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis. International Food Research Journal, 21 (6). pp. 2279-2284. ISSN 1985-4668 E-ISSN 2231-7546 http://www.ifrj.upm.edu.my/21%20%2806%29%202014/31%20IFRJ%2021%20%2806%29%202014%20Intan%20083.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	S Agriculture (General)
spellingShingle	S Agriculture (General) Shahdan, Intan Azura Rahman, Mohammad Tariqur Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis
description	The effectiveness of poultry stunning in producing swift slaughtering was analysed in response to the time needed for the chickens to become insensible upon neck cutting (Td) and the induction of myofiber apoptosis. In total, 49 chicken broilers (BW of 2.17 ± .24 kg) were sacrificed with pre-slaughter stunning, using a constant voltage stunner where the electric current varied between 7.2 to 124.3 mA, and without stunning. The electric current applied during stunning was found to have no effect on Td. Number of apoptotic myonuclei did not vary among stunned and unstunned meat. Apoptosis inducing factor (AIF) and caspase 3 expressions were also not detected in the meat samples of both stunned and unstunned groups at 1 d postmortem. Since the slaughtering process and stunning are associated with stress, the expression of 70 kDa-heat shock protein (Hsp70) was investigated. Moreover Hsp70 is also an inhibitor of apoptosis, by preventing the activation of AIF and apoptosome which stimulates caspase 3 activation. However, expression of Hsp70 was not induced in both stunned groups and unstunned groups. Together, this study found that poultry stunning does not affect Td and myofiber apoptosis.
format	Article
author	Shahdan, Intan Azura Rahman, Mohammad Tariqur
author_facet	Shahdan, Intan Azura Rahman, Mohammad Tariqur
author_sort	Shahdan, Intan Azura
title	Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis
title_short	Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis
title_full	Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis
title_fullStr	Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis
title_full_unstemmed	Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis
title_sort	effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis
publisher	Universiti Putra Malaysia
publishDate	2014
url	http://irep.iium.edu.my/41256/ http://irep.iium.edu.my/41256/ http://irep.iium.edu.my/41256/1/31_IFRJ_21_%2806%29_2014_Intan_083.pdf http://irep.iium.edu.my/41256/6/41256_Effect%20of%20pre-slaughter_scopus.pdf
first_indexed	2023-09-18T20:59:06Z
last_indexed	2023-09-18T20:59:06Z
_version_	1777410502755876864

Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis

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