Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering

Stem cell has been widely researched during the last decade due to its potential to differentiate into many cell types. This study attempts to compare the growth of bone marrow mesenchymal stem cells (BMMSC) aspirated from rabbits’ iliac crest and femur in two culture media i.e. (1) α-Minimum Essent...

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Main Authors:	Ahmad Radzi, Muhammad Aa'zamuddin, Jamaludin, Fatin Nabila, Abdul Rahman, Rozlin, Md Nazir, Noorhidayah, Mohamad Sukri, Norhamiza, Mohammad, Mohd Yusof, Zulkifly, Ahmad Hafiz, Che Ahmad, Aminudin, Abdul Rahman, Suzanah, Hashi, Abdurezak Abdulahi, Sha'ban, Munirah
Format:	Article
Language:	English
Published:	Tissue Engineering and Regenerative Medicine Society of Malaysia 2014
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/41425/ http://irep.iium.edu.my/41425/ http://irep.iium.edu.my/41425/1/RR-181114-068.pdf

id	iium-41425
recordtype	eprints
spelling	iium-414252015-02-12T08:09:19Z http://irep.iium.edu.my/41425/ Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering Ahmad Radzi, Muhammad Aa'zamuddin Jamaludin, Fatin Nabila Abdul Rahman, Rozlin Md Nazir, Noorhidayah Mohamad Sukri, Norhamiza Mohammad, Mohd Yusof Zulkifly, Ahmad Hafiz Che Ahmad, Aminudin Abdul Rahman, Suzanah Hashi, Abdurezak Abdulahi Sha'ban, Munirah R Medicine (General) Stem cell has been widely researched during the last decade due to its potential to differentiate into many cell types. This study attempts to compare the growth of bone marrow mesenchymal stem cells (BMMSC) aspirated from rabbits’ iliac crest and femur in two culture media i.e. (1) α-Minimum Essential Medium (α-MEM) and, (2) the mixture of Ham F12 Nutrient Mixture and Dulbecco’s Modified Eagle Medium (FD). Growth kinetics assessment used in this study includes cell viability, total cell yield, growth rate, population doubling time and total number of cell doubling. FD was found to be an appropriate culture medium to promote better BMMSC growth. BMMSC from iliac crest region showed better proliferation potential than the femur region. Hence, it can be inferred from this study that BMMSC harvested from the iliac crest site and cultured in FD medium is better than femur samples in terms of growth profile. Tissue Engineering and Regenerative Medicine Society of Malaysia 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/41425/1/RR-181114-068.pdf Ahmad Radzi, Muhammad Aa'zamuddin and Jamaludin, Fatin Nabila and Abdul Rahman, Rozlin and Md Nazir, Noorhidayah and Mohamad Sukri, Norhamiza and Mohammad, Mohd Yusof and Zulkifly, Ahmad Hafiz and Che Ahmad, Aminudin and Abdul Rahman, Suzanah and Hashi, Abdurezak Abdulahi and Sha'ban, Munirah (2014) Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering. Regenerative Research, 3 (2). pp. 118-119. ISSN 2232-0822 (Online) http://regres.tesma.org.my/pdf/RR-181114-068.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Ahmad Radzi, Muhammad Aa'zamuddin Jamaludin, Fatin Nabila Abdul Rahman, Rozlin Md Nazir, Noorhidayah Mohamad Sukri, Norhamiza Mohammad, Mohd Yusof Zulkifly, Ahmad Hafiz Che Ahmad, Aminudin Abdul Rahman, Suzanah Hashi, Abdurezak Abdulahi Sha'ban, Munirah Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering
description	Stem cell has been widely researched during the last decade due to its potential to differentiate into many cell types. This study attempts to compare the growth of bone marrow mesenchymal stem cells (BMMSC) aspirated from rabbits’ iliac crest and femur in two culture media i.e. (1) α-Minimum Essential Medium (α-MEM) and, (2) the mixture of Ham F12 Nutrient Mixture and Dulbecco’s Modified Eagle Medium (FD). Growth kinetics assessment used in this study includes cell viability, total cell yield, growth rate, population doubling time and total number of cell doubling. FD was found to be an appropriate culture medium to promote better BMMSC growth. BMMSC from iliac crest region showed better proliferation potential than the femur region. Hence, it can be inferred from this study that BMMSC harvested from the iliac crest site and cultured in FD medium is better than femur samples in terms of growth profile.
format	Article
author	Ahmad Radzi, Muhammad Aa'zamuddin Jamaludin, Fatin Nabila Abdul Rahman, Rozlin Md Nazir, Noorhidayah Mohamad Sukri, Norhamiza Mohammad, Mohd Yusof Zulkifly, Ahmad Hafiz Che Ahmad, Aminudin Abdul Rahman, Suzanah Hashi, Abdurezak Abdulahi Sha'ban, Munirah
author_facet	Ahmad Radzi, Muhammad Aa'zamuddin Jamaludin, Fatin Nabila Abdul Rahman, Rozlin Md Nazir, Noorhidayah Mohamad Sukri, Norhamiza Mohammad, Mohd Yusof Zulkifly, Ahmad Hafiz Che Ahmad, Aminudin Abdul Rahman, Suzanah Hashi, Abdurezak Abdulahi Sha'ban, Munirah
author_sort	Ahmad Radzi, Muhammad Aa'zamuddin
title	Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering
title_short	Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering
title_full	Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering
title_fullStr	Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering
title_full_unstemmed	Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering
title_sort	evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering
publisher	Tissue Engineering and Regenerative Medicine Society of Malaysia
publishDate	2014
url	http://irep.iium.edu.my/41425/ http://irep.iium.edu.my/41425/ http://irep.iium.edu.my/41425/1/RR-181114-068.pdf
first_indexed	2023-09-18T20:59:20Z
last_indexed	2023-09-18T20:59:20Z
_version_	1777410517818671104

Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering

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