Cartilaginous markers expression in human articular chondrocytes overexpressed with SOX9 gene

SOX9 is an essential transcription factor for chondrocyte differentiation and cartilage formation. The limited regenerative capacity of cartilage has led to the development of various treatment modality including gene transfer approaches. In this study, we transiently overexpressed or transfected SO...

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Main Authors:	Mohamad Sukri, Norhamiza, Mat Jusoh, Nurul Syamimi, Mohadis, Hanisah Mohd, Md Nazir, Noorhidayah, Abdul Rahman, Rozlin, Ahmad Radzi, Muhammad Aa'zamuddin, Zulkifly, Ahmad Hafiz, Hashi, Abdurezak Abdulahi, Mohammad, Mohd Yusof, Sha'ban, Munirah
Format:	Article
Language:	English
Published:	Tissue Engineering and Regenerative Medicine Society of Malaysia 2014
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/41426/ http://irep.iium.edu.my/41426/ http://irep.iium.edu.my/41426/1/RR-181114-074.pdf

id	iium-41426
recordtype	eprints
spelling	iium-414262015-02-12T07:25:25Z http://irep.iium.edu.my/41426/ Cartilaginous markers expression in human articular chondrocytes overexpressed with SOX9 gene Mohamad Sukri, Norhamiza Mat Jusoh, Nurul Syamimi Mohadis, Hanisah Mohd Md Nazir, Noorhidayah Abdul Rahman, Rozlin Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Hashi, Abdurezak Abdulahi Mohammad, Mohd Yusof Sha'ban, Munirah R Medicine (General) SOX9 is an essential transcription factor for chondrocyte differentiation and cartilage formation. The limited regenerative capacity of cartilage has led to the development of various treatment modality including gene transfer approaches. In this study, we transiently overexpressed or transfected SOX9 gene in articular chondrocytes derived from consented patients after joint surgery. The aim was to achieve optimum transfection effect by optimizing the lipofection procedure using various DNA concentrations. Chondrocytes were overexpressed with 2.0μg, 4.0μg and 6.0μg of pcDNA3-SOX9. The transfection effect on chondrocytes was assessed through the expression of collagen type II, aggrecan, SOX9 and collagen type I using the two-step reverse-transcriptase polymerase chain reaction (RT-PCR). Results showed that at low DNA concentration, the SOX9 transfection up-regulates all cartilage-specific markers expression, but down-regulates collagen type I. Hence, optimum transfection effect can be achieved using low DNA concentration. The ability to sustain the cartilage-specific markers indicates potential implications in cartilage engineering. Tissue Engineering and Regenerative Medicine Society of Malaysia 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/41426/1/RR-181114-074.pdf Mohamad Sukri, Norhamiza and Mat Jusoh, Nurul Syamimi and Mohadis, Hanisah Mohd and Md Nazir, Noorhidayah and Abdul Rahman, Rozlin and Ahmad Radzi, Muhammad Aa'zamuddin and Zulkifly, Ahmad Hafiz and Hashi, Abdurezak Abdulahi and Mohammad, Mohd Yusof and Sha'ban, Munirah (2014) Cartilaginous markers expression in human articular chondrocytes overexpressed with SOX9 gene. Regenerative Research, 3 (2). pp. 131-132. ISSN 2232-0822 (Online) http://www.regres.tesma.org.my/pdf/RR-181114-074.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Mohamad Sukri, Norhamiza Mat Jusoh, Nurul Syamimi Mohadis, Hanisah Mohd Md Nazir, Noorhidayah Abdul Rahman, Rozlin Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Hashi, Abdurezak Abdulahi Mohammad, Mohd Yusof Sha'ban, Munirah Cartilaginous markers expression in human articular chondrocytes overexpressed with SOX9 gene
description	SOX9 is an essential transcription factor for chondrocyte differentiation and cartilage formation. The limited regenerative capacity of cartilage has led to the development of various treatment modality including gene transfer approaches. In this study, we transiently overexpressed or transfected SOX9 gene in articular chondrocytes derived from consented patients after joint surgery. The aim was to achieve optimum transfection effect by optimizing the lipofection procedure using various DNA concentrations. Chondrocytes were overexpressed with 2.0μg, 4.0μg and 6.0μg of pcDNA3-SOX9. The transfection effect on chondrocytes was assessed through the expression of collagen type II, aggrecan, SOX9 and collagen type I using the two-step reverse-transcriptase polymerase chain reaction (RT-PCR). Results showed that at low DNA concentration, the SOX9 transfection up-regulates all cartilage-specific markers expression, but down-regulates collagen type I. Hence, optimum transfection effect can be achieved using low DNA concentration. The ability to sustain the cartilage-specific markers indicates potential implications in cartilage engineering.
format	Article
author	Mohamad Sukri, Norhamiza Mat Jusoh, Nurul Syamimi Mohadis, Hanisah Mohd Md Nazir, Noorhidayah Abdul Rahman, Rozlin Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Hashi, Abdurezak Abdulahi Mohammad, Mohd Yusof Sha'ban, Munirah
author_facet	Mohamad Sukri, Norhamiza Mat Jusoh, Nurul Syamimi Mohadis, Hanisah Mohd Md Nazir, Noorhidayah Abdul Rahman, Rozlin Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Hashi, Abdurezak Abdulahi Mohammad, Mohd Yusof Sha'ban, Munirah
author_sort	Mohamad Sukri, Norhamiza
title	Cartilaginous markers expression in human articular chondrocytes overexpressed with SOX9 gene
title_short	Cartilaginous markers expression in human articular chondrocytes overexpressed with SOX9 gene
title_full	Cartilaginous markers expression in human articular chondrocytes overexpressed with SOX9 gene
title_fullStr	Cartilaginous markers expression in human articular chondrocytes overexpressed with SOX9 gene
title_full_unstemmed	Cartilaginous markers expression in human articular chondrocytes overexpressed with SOX9 gene
title_sort	cartilaginous markers expression in human articular chondrocytes overexpressed with sox9 gene
publisher	Tissue Engineering and Regenerative Medicine Society of Malaysia
publishDate	2014
url	http://irep.iium.edu.my/41426/ http://irep.iium.edu.my/41426/ http://irep.iium.edu.my/41426/1/RR-181114-074.pdf
first_indexed	2023-09-18T20:59:20Z
last_indexed	2023-09-18T20:59:20Z
_version_	1777410517966520320

Cartilaginous markers expression in human articular chondrocytes overexpressed with SOX9 gene

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