Development and validation of new economic HPLC method for the analysis of gliclazide in tablet dosage form

New sensitive and highly economic analytical method for the analysis of gliclazide in tablet dosage form was developed and validated using UHPLC column and HPLC instrument. Standards and samples were prepared by dissolving gliclazide standard or gliclazide tablets in small amount of methanol sonicat...

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Main Authors:	Helaluddin , Abul Bashar Mohammed, Alaama, Mohammed, Mohamad , Huda Jamilah, Amiruddin , Noor Syafawati, Abbas, Syed Atif
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	QD Chemistry RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/41457/ http://irep.iium.edu.my/41457/ http://irep.iium.edu.my/41457/1/18_MICC.pdf

id	iium-41457
recordtype	eprints
spelling	iium-414572015-04-23T06:31:33Z http://irep.iium.edu.my/41457/ Development and validation of new economic HPLC method for the analysis of gliclazide in tablet dosage form Helaluddin , Abul Bashar Mohammed Alaama, Mohammed Mohamad , Huda Jamilah Amiruddin , Noor Syafawati Abbas, Syed Atif QD Chemistry RS Pharmacy and materia medica New sensitive and highly economic analytical method for the analysis of gliclazide in tablet dosage form was developed and validated using UHPLC column and HPLC instrument. Standards and samples were prepared by dissolving gliclazide standard or gliclazide tablets in small amount of methanol sonicate for 5 min then top up using mobile phase. Samples were analysed using Agilent 1200 Series RP-HPLC equipped with quaternary pump and auto injector. Samples were injected to C18 HSS water column (2.1X100 mm, 2.5µm) and the mobile phase consisted of triethylamine buffer (pH=3 adjusted using phosphoric acid) and acetonitrile in the ratio 50:50 v/. The flow rate was 0.4 ml/min and UV detector was used for the detection and the wavelength was 229 nm. The method was validated according to ICH guidelines. The retention time for gliclazide peak was 3.437±0.41 min with the total run time of 6 min with total solvent consumption of 2.4 ml per run. The method was found linear over the range 0.1-40 µg/ml with correlation coefficient R2 = 0.999. The recovery was 98.09-100.19 %, and the method showed high precision and repeatability. All validated parameters were in the acceptable range of ICH requirements. In conclusion new rapid sensitive and highly economic method was developed and validated for the analysis of gliclazide in tablet dosage form. 2014 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/41457/1/18_MICC.pdf Helaluddin , Abul Bashar Mohammed and Alaama, Mohammed and Mohamad , Huda Jamilah and Amiruddin , Noor Syafawati and Abbas, Syed Atif (2014) Development and validation of new economic HPLC method for the analysis of gliclazide in tablet dosage form. In: Malaysian International Chemical Congress (18MICC) 2014, 3-5 Nov, 2014, Putra World Trade Centre, Kuala Lumpur. http://www.18micc.org.my/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry RS Pharmacy and materia medica
spellingShingle	QD Chemistry RS Pharmacy and materia medica Helaluddin , Abul Bashar Mohammed Alaama, Mohammed Mohamad , Huda Jamilah Amiruddin , Noor Syafawati Abbas, Syed Atif Development and validation of new economic HPLC method for the analysis of gliclazide in tablet dosage form
description	New sensitive and highly economic analytical method for the analysis of gliclazide in tablet dosage form was developed and validated using UHPLC column and HPLC instrument. Standards and samples were prepared by dissolving gliclazide standard or gliclazide tablets in small amount of methanol sonicate for 5 min then top up using mobile phase. Samples were analysed using Agilent 1200 Series RP-HPLC equipped with quaternary pump and auto injector. Samples were injected to C18 HSS water column (2.1X100 mm, 2.5µm) and the mobile phase consisted of triethylamine buffer (pH=3 adjusted using phosphoric acid) and acetonitrile in the ratio 50:50 v/. The flow rate was 0.4 ml/min and UV detector was used for the detection and the wavelength was 229 nm. The method was validated according to ICH guidelines. The retention time for gliclazide peak was 3.437±0.41 min with the total run time of 6 min with total solvent consumption of 2.4 ml per run. The method was found linear over the range 0.1-40 µg/ml with correlation coefficient R2 = 0.999. The recovery was 98.09-100.19 %, and the method showed high precision and repeatability. All validated parameters were in the acceptable range of ICH requirements. In conclusion new rapid sensitive and highly economic method was developed and validated for the analysis of gliclazide in tablet dosage form.
format	Conference or Workshop Item
author	Helaluddin , Abul Bashar Mohammed Alaama, Mohammed Mohamad , Huda Jamilah Amiruddin , Noor Syafawati Abbas, Syed Atif
author_facet	Helaluddin , Abul Bashar Mohammed Alaama, Mohammed Mohamad , Huda Jamilah Amiruddin , Noor Syafawati Abbas, Syed Atif
author_sort	Helaluddin , Abul Bashar Mohammed
title	Development and validation of new economic HPLC method for the analysis of gliclazide in tablet dosage form
title_short	Development and validation of new economic HPLC method for the analysis of gliclazide in tablet dosage form
title_full	Development and validation of new economic HPLC method for the analysis of gliclazide in tablet dosage form
title_fullStr	Development and validation of new economic HPLC method for the analysis of gliclazide in tablet dosage form
title_full_unstemmed	Development and validation of new economic HPLC method for the analysis of gliclazide in tablet dosage form
title_sort	development and validation of new economic hplc method for the analysis of gliclazide in tablet dosage form
publishDate	2014
url	http://irep.iium.edu.my/41457/ http://irep.iium.edu.my/41457/ http://irep.iium.edu.my/41457/1/18_MICC.pdf
first_indexed	2023-09-18T20:59:22Z
last_indexed	2023-09-18T20:59:22Z
_version_	1777410520164335616

Development and validation of new economic HPLC method for the analysis of gliclazide in tablet dosage form

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