Preparation and characterization of nigella sativa microemulsions

The aims of this study were to develop and characterize an oil-in-water (o/w) Nigella sativa (N. sativa) microemulsion. The microemulsions were prepared by drop-wise titration of N. sativa oil into mixtures of surfactant blends (Span 20, Span 80, Tween 20, Tween 80, Tween 85) and water. All transpar...

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Main Authors:	Mohd Nor, Nurul Hafizah, Mohd Shafri, Mohd Affendi, Mohamed, Farahidah
Format:	Article
Language:	English English
Published:	Academic Sciences 2014
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/41553/ http://irep.iium.edu.my/41553/ http://irep.iium.edu.my/41553/1/41553_Preparation%20and%20characterization%20of%20nigella%20sativa.pdf http://irep.iium.edu.my/41553/2/41553_Preparation%20and%20characterization%20of%20nigella%20sativa_SCOPUS.pdf

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spelling	iium-415532017-09-19T06:25:01Z http://irep.iium.edu.my/41553/ Preparation and characterization of nigella sativa microemulsions Mohd Nor, Nurul Hafizah Mohd Shafri, Mohd Affendi Mohamed, Farahidah RS Pharmacy and materia medica The aims of this study were to develop and characterize an oil-in-water (o/w) Nigella sativa (N. sativa) microemulsion. The microemulsions were prepared by drop-wise titration of N. sativa oil into mixtures of surfactant blends (Span 20, Span 80, Tween 20, Tween 80, Tween 85) and water. All transparent ternary mixtures were characterized for their viscosity and droplet size. The stability of the microemulsion was evaluated by subjecting them to stressful conditions, namely centrifugation (2000 g for 20 minutes) and heating in a drying oven (60 °C to 105 °C for 5 hours) and the droplet size was determined following one month storage at room temperature (25 °C) thereafter. Based on the results, a phase diagram was constructed from corresponding volumes of those 3 components. N. sativa mixtures (ranging from 7.4% to 10.7%) prepared at HLB 16 of surfactant blends (Tween 20: Tween 80; 6:4) with water (ranging from 17.9% to 18.5%) yielded transparent liquids. The constructed phase diagram displayed regions of a few types of microemulsion and emulsion. Interestingly, droplet size of freshly prepared mixtures was wider in range (5 to 15.6 nm) than the size following stressful condition (11.3 to 12.4 nm). It was concluded that N. sativa oil could be formulated into microemulsion at specific HLB value of surfactant blends. Such system was envisaged to enable routine rapid in vitro test on neuron cell lines loaded with N. sativa oil or possibly other lipophilic materials whenever viewing of neurite extension is required. Keywords: Nigella sativa, Microemulsions, Sorbitan-based surfactants, Phase diagram Academic Sciences 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/41553/1/41553_Preparation%20and%20characterization%20of%20nigella%20sativa.pdf application/pdf en http://irep.iium.edu.my/41553/2/41553_Preparation%20and%20characterization%20of%20nigella%20sativa_SCOPUS.pdf Mohd Nor, Nurul Hafizah and Mohd Shafri, Mohd Affendi and Mohamed, Farahidah (2014) Preparation and characterization of nigella sativa microemulsions. International Journal of Pharmacy and Pharmaceutical Sciences, 6 (9). pp. 485-489. ISSN 0975-1491 http://www.ijppsjournal.com/contents.htm
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Mohd Nor, Nurul Hafizah Mohd Shafri, Mohd Affendi Mohamed, Farahidah Preparation and characterization of nigella sativa microemulsions
description	The aims of this study were to develop and characterize an oil-in-water (o/w) Nigella sativa (N. sativa) microemulsion. The microemulsions were prepared by drop-wise titration of N. sativa oil into mixtures of surfactant blends (Span 20, Span 80, Tween 20, Tween 80, Tween 85) and water. All transparent ternary mixtures were characterized for their viscosity and droplet size. The stability of the microemulsion was evaluated by subjecting them to stressful conditions, namely centrifugation (2000 g for 20 minutes) and heating in a drying oven (60 °C to 105 °C for 5 hours) and the droplet size was determined following one month storage at room temperature (25 °C) thereafter. Based on the results, a phase diagram was constructed from corresponding volumes of those 3 components. N. sativa mixtures (ranging from 7.4% to 10.7%) prepared at HLB 16 of surfactant blends (Tween 20: Tween 80; 6:4) with water (ranging from 17.9% to 18.5%) yielded transparent liquids. The constructed phase diagram displayed regions of a few types of microemulsion and emulsion. Interestingly, droplet size of freshly prepared mixtures was wider in range (5 to 15.6 nm) than the size following stressful condition (11.3 to 12.4 nm). It was concluded that N. sativa oil could be formulated into microemulsion at specific HLB value of surfactant blends. Such system was envisaged to enable routine rapid in vitro test on neuron cell lines loaded with N. sativa oil or possibly other lipophilic materials whenever viewing of neurite extension is required. Keywords: Nigella sativa, Microemulsions, Sorbitan-based surfactants, Phase diagram
format	Article
author	Mohd Nor, Nurul Hafizah Mohd Shafri, Mohd Affendi Mohamed, Farahidah
author_facet	Mohd Nor, Nurul Hafizah Mohd Shafri, Mohd Affendi Mohamed, Farahidah
author_sort	Mohd Nor, Nurul Hafizah
title	Preparation and characterization of nigella sativa microemulsions
title_short	Preparation and characterization of nigella sativa microemulsions
title_full	Preparation and characterization of nigella sativa microemulsions
title_fullStr	Preparation and characterization of nigella sativa microemulsions
title_full_unstemmed	Preparation and characterization of nigella sativa microemulsions
title_sort	preparation and characterization of nigella sativa microemulsions
publisher	Academic Sciences
publishDate	2014
url	http://irep.iium.edu.my/41553/ http://irep.iium.edu.my/41553/ http://irep.iium.edu.my/41553/1/41553_Preparation%20and%20characterization%20of%20nigella%20sativa.pdf http://irep.iium.edu.my/41553/2/41553_Preparation%20and%20characterization%20of%20nigella%20sativa_SCOPUS.pdf
first_indexed	2023-09-18T20:59:29Z
last_indexed	2023-09-18T20:59:29Z
_version_	1777410527554699264

Preparation and characterization of nigella sativa microemulsions

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