Tissue-engineered human articular cartilage demonstrates intense immunopositivity for collagen type II

Tissue engineering is known as an interdisciplinary biomedical field that applies the principles and method of engineering and life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function. Our laboratory has successfully cultured human articular c...

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Main Authors:	Sha'ban, Munirah, Saim, Aminuddin, Chua, Kien Hui, Osman Cassim, Samsudin, M. Yusuf, Badrul Akmal Hisham, Baharudin, Azmi, Hussien, Fuzina Nor, Idrus, Ruszymah
Format:	Article
Language:	English
Published:	Penerbit Universiti Sains Malaysia 2006
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/41850/ http://irep.iium.edu.my/41850/ http://irep.iium.edu.my/41850/1/17-01-02Ruszymah.pdf

id	iium-41850
recordtype	eprints
spelling	iium-418502015-06-29T02:53:48Z http://irep.iium.edu.my/41850/ Tissue-engineered human articular cartilage demonstrates intense immunopositivity for collagen type II Sha'ban, Munirah Saim, Aminuddin Chua, Kien Hui Osman Cassim, Samsudin M. Yusuf, Badrul Akmal Hisham Baharudin, Azmi Hussien, Fuzina Nor Idrus, Ruszymah R Medicine (General) Tissue engineering is known as an interdisciplinary biomedical field that applies the principles and method of engineering and life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function. Our laboratory has successfully cultured human articular chondrocytes and formed tissue-engineered cartilage, which is reliable for replacement of damaged tissue. Yet, we still need to analyse whether the tissue-engineered cartilage has similarity to the native cartilage. This study was designed to evaluate the quality of human articular cartilage using immunohistochemistry (IHC) technique. Human articular cartilages were obtained from excessive tissues from consented patients after joint surgery. Chondrocytes were isolated from tissue via enzymatic digestion and cultured in growth media for several passages to obtain sufficient cells. The cells were then transformed into three-dimensional in vitro constructs using biodegradable scaffold and were placed subcutaneously in the nude mice. Constructs were harvested after 8 weeks and were analysed by IHC technique to detect the presence of collagen type I, collagen type II and fibrocytes surface protein. The results showed that stable in vitro construct mature into tissue-engineered cartilage after implantation, which grossly resembled native cartilage. In vitro construct strongly expressed collagen type I, collagen type II and the fibrocytes surface protein at the pericellular. In vivo construct showed the distribution of collagen type II expression throughout the tissue, which denotes good quality hyaline cartilage. In conclusion, we have successfully engineered human articular cartilage which resembles the native tissue by IHC evaluation technique. Penerbit Universiti Sains Malaysia 2006 Article PeerReviewed application/pdf en http://irep.iium.edu.my/41850/1/17-01-02Ruszymah.pdf Sha'ban, Munirah and Saim, Aminuddin and Chua, Kien Hui and Osman Cassim, Samsudin and M. Yusuf, Badrul Akmal Hisham and Baharudin, Azmi and Hussien, Fuzina Nor and Idrus, Ruszymah (2006) Tissue-engineered human articular cartilage demonstrates intense immunopositivity for collagen type II. Tropical Life Sciences Research, 17 (1). pp. 9-18. ISSN 1985-3718 http://www.tlsr.usm.my/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Sha'ban, Munirah Saim, Aminuddin Chua, Kien Hui Osman Cassim, Samsudin M. Yusuf, Badrul Akmal Hisham Baharudin, Azmi Hussien, Fuzina Nor Idrus, Ruszymah Tissue-engineered human articular cartilage demonstrates intense immunopositivity for collagen type II
description	Tissue engineering is known as an interdisciplinary biomedical field that applies the principles and method of engineering and life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function. Our laboratory has successfully cultured human articular chondrocytes and formed tissue-engineered cartilage, which is reliable for replacement of damaged tissue. Yet, we still need to analyse whether the tissue-engineered cartilage has similarity to the native cartilage. This study was designed to evaluate the quality of human articular cartilage using immunohistochemistry (IHC) technique. Human articular cartilages were obtained from excessive tissues from consented patients after joint surgery. Chondrocytes were isolated from tissue via enzymatic digestion and cultured in growth media for several passages to obtain sufficient cells. The cells were then transformed into three-dimensional in vitro constructs using biodegradable scaffold and were placed subcutaneously in the nude mice. Constructs were harvested after 8 weeks and were analysed by IHC technique to detect the presence of collagen type I, collagen type II and fibrocytes surface protein. The results showed that stable in vitro construct mature into tissue-engineered cartilage after implantation, which grossly resembled native cartilage. In vitro construct strongly expressed collagen type I, collagen type II and the fibrocytes surface protein at the pericellular. In vivo construct showed the distribution of collagen type II expression throughout the tissue, which denotes good quality hyaline cartilage. In conclusion, we have successfully engineered human articular cartilage which resembles the native tissue by IHC evaluation technique.
format	Article
author	Sha'ban, Munirah Saim, Aminuddin Chua, Kien Hui Osman Cassim, Samsudin M. Yusuf, Badrul Akmal Hisham Baharudin, Azmi Hussien, Fuzina Nor Idrus, Ruszymah
author_facet	Sha'ban, Munirah Saim, Aminuddin Chua, Kien Hui Osman Cassim, Samsudin M. Yusuf, Badrul Akmal Hisham Baharudin, Azmi Hussien, Fuzina Nor Idrus, Ruszymah
author_sort	Sha'ban, Munirah
title	Tissue-engineered human articular cartilage demonstrates intense immunopositivity for collagen type II
title_short	Tissue-engineered human articular cartilage demonstrates intense immunopositivity for collagen type II
title_full	Tissue-engineered human articular cartilage demonstrates intense immunopositivity for collagen type II
title_fullStr	Tissue-engineered human articular cartilage demonstrates intense immunopositivity for collagen type II
title_full_unstemmed	Tissue-engineered human articular cartilage demonstrates intense immunopositivity for collagen type II
title_sort	tissue-engineered human articular cartilage demonstrates intense immunopositivity for collagen type ii
publisher	Penerbit Universiti Sains Malaysia
publishDate	2006
url	http://irep.iium.edu.my/41850/ http://irep.iium.edu.my/41850/ http://irep.iium.edu.my/41850/1/17-01-02Ruszymah.pdf
first_indexed	2023-09-18T20:59:48Z
last_indexed	2023-09-18T20:59:48Z
_version_	1777410546671288320

Tissue-engineered human articular cartilage demonstrates intense immunopositivity for collagen type II

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